Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes
Nicolas Renier,Eliza L. Adams,Christoph Kirst,Zhuhao Wu,Ricardo Azevedo,Johannes Kohl,Anita E. Autry,Lolahon R. Kadiri,Kannan Umadevi Venkataraju,Yu Zhou,Victoria X. Wang,Cheuk Y. Tang,Olav Olsen,Catherine Dulac,Pavel Osten,Marc Tessier-Lavigne +15 more
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TLDR
A pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program that is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.About:
This article is published in Cell.The article was published on 2016-06-16 and is currently open access. It has received 581 citations till now.read more
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Neuroinvasion of SARS-CoV-2 in human and mouse brain.
Eric Song,Ce Zhang,Benjamin Israelow,Alice Lu-Culligan,Alba Vieites Prado,Sophie Skriabine,Peiwen Lu,Orr-El Weizman,Feimei Liu,Yile Dai,Klara Szigeti-Buck,Yuki Yasumoto,Guilin Wang,Christopher Castaldi,Jaime Heltke,Evelyn Ng,John Wheeler,Mia Madel Alfajaro,Etienne Levavasseur,Benjamin Fontes,Neal G. Ravindra,David van Dijk,Shrikant Mane,Murat Gunel,Aaron M. Ring,Syed A. Jaffar Kazmi,Kai Zhang,Craig B. Wilen,Tamas L. Horvath,Isabelle Plu,Stéphane Haïk,Jean-Leon Thomas,Jean-Leon Thomas,Angeliki Louvi,Shelli F. Farhadian,Anita Huttner,Danielle Seilhean,Nicolas Renier,Kaya Bilguvar,Akiko Iwasaki,Akiko Iwasaki +40 more
Abstract: Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there is no consensus on the consequences of CNS infections. Here, we used three independent approaches to probe the capacity of SARS-CoV-2 to infect the brain. First, using human brain organoids, we observed clear evidence of infection with accompanying metabolic changes in infected and neighboring neurons. However, no evidence for type I interferon responses was detected. We demonstrate that neuronal infection can be prevented by blocking ACE2 with antibodies or by administering cerebrospinal fluid from a COVID-19 patient. Second, using mice overexpressing human ACE2, we demonstrate SARS-CoV-2 neuroinvasion in vivo. Finally, in autopsies from patients who died of COVID-19, we detect SARS-CoV-2 in cortical neurons and note pathological features associated with infection with minimal immune cell infiltrates. These results provide evidence for the neuroinvasive capacity of SARS-CoV-2 and an unexpected consequence of direct infection of neurons by SARS-CoV-2.
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The Allen Mouse Brain Common Coordinate Framework: A 3D Reference Atlas.
Quanxin Wang,Songlin Ding,Yang Li,Josh Royall,David Feng,Phil Lesnar,Nile Graddis,Maitham Naeemi,Benjamin A.C. Facer,Anh Ho,Tim A. Dolbeare,Brandon Blanchard,Nick Dee,Wayne Wakeman,Karla E. Hirokawa,Aaron Szafer,Susan M. Sunkin,Seung Wook Oh,Amy Bernard,John W. Phillips,Michael Hawrylycz,Christof Koch,Hongkui Zeng,Julie A. Harris,Lydia Ng +24 more
TL;DR: This work constructed an average template brain at 10 μm voxel resolution by interpolating high resolution in-plane serial two-photon tomography images with 100 μm z-sampling from 1,675 young adult C57BL/6J mice and parcellated the entire brain directly in 3D.
Journal ArticleDOI
Shrinkage-mediated imaging of entire organs and organisms using uDISCO
Chenchen Pan,Ruiyao Cai,Francesca Paola Quacquarelli,Alireza Ghasemigharagoz,Athanasios Lourbopoulos,Paweł Matryba,Paweł Matryba,Nikolaus Plesnila,Martin Dichgans,Farida Hellal,Ali Ertürk +10 more
TL;DR: The 'ultimate DISCO' (uDISCO) clearing is developed to overcome limitations in volumetric imaging and preserves fluorescent proteins over months and renders intact organs and rodent bodies transparent while reducing their size up to 65%.
Journal ArticleDOI
An atlas of the protein-coding genes in the human, pig, and mouse brain
Evelina Sjöstedt,Evelina Sjöstedt,Wen Zhong,Linn Fagerberg,Max J. Karlsson,Nicholas Mitsios,Csaba Adori,Per Oksvold,Fredrik Edfors,Agnieszka Limiszewska,Feria Hikmet,Jinrong Huang,Yutao Du,Lin Lin,Zhanying Dong,Ling Yang,Xin Liu,Hui Jiang,Xun Xu,Jian Wang,Huanming Yang,Lars Bolund,Adil Mardinoglu,Cheng Zhang,Kalle von Feilitzen,Cecilia Lindskog,Fredrik Pontén,Yonglun Luo,Tomas Hökfelt,Mathias Uhlén,Mathias Uhlén,Jan Mulder +31 more
TL;DR: A comprehensive molecular dissection of the main regions of the human, pig, and mouse brain using transcriptomics and antibody-based mapping suggests similar regional organization and expression patterns in the three mammalian species, consistent with the view that basic brain architecture is preserved during mammalian evolution.
Journal ArticleDOI
Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex.
Sinisa Hrvatin,Daniel Hochbaum,Daniel Hochbaum,M. Aurel Nagy,Marcelo Cicconet,Keiramarie Robertson,Lucas Cheadle,Rapolas Zilionis,Rapolas Zilionis,Alex Ratner,Rebeca Borges-Monroy,Allon M. Klein,Bernardo L. Sabatini,Michael E. Greenberg +13 more
TL;DR: The results reveal the dynamic landscape of the stimulus-dependent transcriptional changes occurring across cell types in the visual cortex; these changes are probably critical for cortical function and may be sites of deregulation in developmental brain disorders.
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