Institution
Centre for Cellular and Molecular Biology
Facility•Hyderabad, India•
About: Centre for Cellular and Molecular Biology is a facility organization based out in Hyderabad, India. It is known for research contribution in the topics: Population & Gene. The organization has 2439 authors who have published 3193 publications receiving 97833 citations.
Topics: Population, Gene, Membrane, Apoptosis, Mutant
Papers published on a yearly basis
Papers
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TL;DR: The 1-N-phenylnapthylamine (NPN)-uptake assay revealed that the OMVs protect the bacterium from membrane active antibiotics by scavenging them and also showed that membrane and protein packing of the OMV was similar to the parent bacterium.
Abstract: Outer membrane vesicles (OMVs) of Gram-negative bacteria form an important aspect of bacterial physiology as they are involved in various functions essential for their survival. The OMVs of the Ant...
83 citations
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TL;DR: These assays, standardized for toxicity evaluation of “regular” chemicals, proved efficient also for shortlisting of hazardous NMs and are recommended for immunotoxicity evaluation of high aspect ratio NMs (such as MWCNTs).
Abstract: Within EU FP7 project NANOVALID, the (eco)toxicity of 7 well-characterized engineered nanomaterials (NMs) was evaluated by 15 bioassays in 4 laboratories. The highest tested nominal concentration of NMs was 100 mg/l. The panel of the bioassays yielded the following toxicity order: Ag > ZnO > CuO > TiO2 > MWCNTs > SiO2 > Au. Ag, ZnO and CuO proved very toxic in the majority of assays, assumingly due to dissolution. The latter was supported by the parallel analysis of the toxicity of respective soluble metal salts. The most sensitive tests/species were Daphnia magna (towards Ag NMs, 24-h EC50 = 0.003 mg Ag/l), algae Raphidocelis subcapitata (ZnO and CuO, 72-h EC50 = 0.14 mg Zn/l and 0.7 mg Cu/l, respectively) and murine fibroblasts BALB/3T3 (CuO, 48-h EC50 = 0.7 mg Cu/l). MWCNTs showed toxicity only towards rat alveolar macrophages (EC50 = 15.3 mg/l) assumingly due to high aspect ratio and TiO2 towards R. subcapitata (EC50 = 6.8 mg Ti/l) due to agglomeration of TiO2 and entrapment of algal cells. Fi...
82 citations
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TL;DR: It is demonstrated that chronic pancreatitis is a genomic disorder and the molecular basis of 6% of the young ICP patients is revealed and all 5 previously described copy number variations involving PRSS1 or/and PRSS2 are artifacts.
82 citations
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TL;DR: This review aims to describe several representative examples of how the zebrafish can be successfully used to identify novel genes and assign gene function, providing invaluable clues to human pathophysiology.
Abstract: The zebrafish (Danio rerio) has been widely utilised for the study of developmental biology, which has lead to the evolution of sophisticated cellular and molecular approaches. More recently, the rapid progress of various zebrafish genomic infrastructure initiatives is facilitating the development of zebrafish models of human disease. This review aims to describe several representative examples of how the zebrafish can be successfully used to identify novel genes and assign gene function, providing invaluable clues to human pathophysiology.
82 citations
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TL;DR: Results indicate that Tat B disrupts BBB integrity to a greater extent compared to Tat C and cocaine further differentially exacerbates the BBB dysfunction, which may be correlated with the clade-specific differences observed in HIV-1-associated neurological disorders.
Abstract: In recent years, increasing interest has emerged to assess the human immunodeficiency virus type 1 (HIV-1) clade C viral pathogenesis due to its anticipated spread in the United States and other western countries. Previous studies suggest that clade C is less neuropathogenic than clade B; however, the underlying mechanism is poorly understood. Additionally, the interactive role of drugs of abuse such as cocaine on clade C-associated neuropathogenesis has not been reported. In the current study, we hypothesize that HIV-1 clade-specific Tat proteins exert differential effects on blood-brain barrier (BBB) integrity and cocaine further differentially aggravates the BBB dysfunction. We evaluated the effect of Tat B and Tat C and/or cocaine on the BBB integrity using an in vitro model constructed with primary human brain microvascular endothelial cells (HBMECs) and astrocytes. The BBB membrane integrity was measured by transendothelial electrical resistance (TEER) and paracellular permeability was measured by fluorescein isothiocyanate (FITC)-dextran transport assay and monocytes transmigration across the BBB. Results indicate that Tat B disrupts BBB integrity to a greater extent compared to Tat C and cocaine further differentially exacerbates the BBB dysfunction. This BBB dysfunction was associated with altered expression of tight junction proteins zona occuldens (ZO-1) and junctional adhesion molecule (JAM)-2. Thus, these results for the first time delineate the differential role of Tat B and Tat C and/or cocaine in BBB dysfunction, which may be correlated with the clade-specific differences observed in HIV-1-associated neurological disorders.
82 citations
Authors
Showing all 2450 results
Name | H-index | Papers | Citations |
---|---|---|---|
Robert G. Parton | 136 | 459 | 59737 |
Leonard I. Zon | 134 | 642 | 66329 |
Clive Osmond | 131 | 588 | 84694 |
Rajeev K. Varshney | 102 | 709 | 39796 |
David E. James | 96 | 394 | 30260 |
Helga Refsum | 90 | 316 | 37463 |
Ueli Grossniklaus | 88 | 306 | 26673 |
Arvind Kumar | 85 | 876 | 33484 |
Caroline H.D. Fall | 79 | 306 | 40991 |
Pramod K. Srivastava | 79 | 390 | 27330 |
Yau-Huei Wei | 78 | 385 | 22286 |
Stephen Kennedy | 75 | 300 | 17927 |
Frederic Geissmann | 73 | 147 | 37781 |
Toomas Kivisild | 72 | 203 | 22124 |
Geoffrey I. McFadden | 72 | 234 | 21772 |