Centre for Cellular and Molecular Biology

About: Centre for Cellular and Molecular Biology is a facility organization based out in Hyderabad, India. It is known for research contribution in the topics: Population & Gene. The organization has 2439 authors who have published 3193 publications receiving 97833 citations.

Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5.

Abstract: Japanese encephalitis virus (JEV) is a plus strand RNA virus, which infects brain MicroRNAs are regulatory non-coding RNAs which regulate the expression of various genes in cells Viruses modulate the expression of various microRNAs to suppress anti-viral signaling and evade the immune response SOCS (Suppressor of cytokine signalling) family of proteins are negative regulators of anti-viral Jak-STAT pathway In this study, we demonstrated the regulatory role of SOCS5 in Jak-STAT signaling and its exploitation by JEV through a microRNA mediated mechanism JEV infection in human brain microglial cells (CHME3) downregulated the expression of miR-432, and upregulated SOCS5 levels SOCS5 was validated as a target of miR-432 by using 3'UTR clone of SOCS5 in luciferase vector along with miR-432 mimic The overexpression of miR-432 prior to JEV infection enhanced the phosphorylation of STAT1 resulting into increased ISRE activity and cellular inflammatory response resulting into diminished viral replication The knockdown of SOCS5 resulted into increased STAT1 phosphorylation and suppressed viral replication JEV infection mediated downregulation of miR-432 leads to SOCS5 upregulation, which helps the virus to evade cellular anti-viral response This study demonstrated that JEV utilizes this microRNA mediated strategy to manipulate cellular immune response promoting JEV pathogenesis

Psychrobacter vallis sp. nov. and Psychrobacter aquaticus sp. nov., from Antarctica

Abstract: Twelve strains of psychrophilic bacteria were isolated from cyanobacterial mat samples collected from various water bodies in the McMurdo Dry Valley region of Antarctica All the isolates were Gram-negative, non-motile, coccoid, psychrophilic, halotolerant bacteria and had C(16 : 1)omega7c, C(17 : 1)omega8c and C(18 : 1)omega9c as the major fatty acids, ubiquinone-8 as the respiratory quinone and DNA G+C content of 41-46 mol% Based on these characteristics, the isolates were assigned to the genus Psychrobacter Based on their SDS-PAGE profiles, the 12 isolates could be categorized into three groups Six isolates of Group I were identified as representing strains of Psychrobacter okhotskensis However, using detailed phenotypic and chemotaxonomic characteristics and phylogenetic analysis based on their 16S rRNA gene sequences, strain CMS 39(T), the only strain from Group II, and strain CMS 56(T), a representative strain of Group III, were different from each other and from all recognized species of Psychrobacter Therefore, it is proposed to classify CMS 39(T) (=DSM 15337(T)=MTCC 4208(T)) and CMS 56(T) (=DSM 15339(T)=MTCC 4386(T)) as representing the type strains of novel species of Psychrobacter, for which the names Psychrobacter vallis sp nov and Psychrobacter aquaticus sp nov, respectively, are proposed

Molecular characterization of plasmid-encoded blaTEM, blaSHV and blaCTX-M among extended spectrum β-lactamases [ESBLs] producing Acinetobacter baumannii.

Abstract: Acinetobacter baumannii associated with nosocomial infections are among the top six drug resistant microbes. Extensive use of the β-lactam group of antibiotics has resulted in the emergence of drug...