Ensenada Center for Scientific Research and Higher Education

About: Ensenada Center for Scientific Research and Higher Education is a facility organization based out in Ensenada, Mexico. It is known for research contribution in the topics: Population & Nonlinear system. The organization has 1934 authors who have published 3733 publications receiving 63115 citations.

A heterotrimer model of the complete Microprocessor complex revealed by single-molecule subunit counting.

Abstract: During microRNA (miRNA) biogenesis, the Microprocessor complex (MC), composed minimally of Drosha, an RNaseIII enzyme, and DGCR8, a double-stranded RNA-binding protein, cleaves the primary-miRNA (pri-miRNA) to release the pre-miRNA stem-loop structure. Size-exclusion chromatography of the MC, isolated from mammalian cells, suggested multiple copies of one or both proteins in the complex. However, the exact stoichiometry was unknown. Initial experiments suggested that DGCR8 bound pri-miRNA substrates specifically, and given that Drosha could not be bound or cross-linked to RNA, a sequential model for binding was established in which DGCR8 bound first and recruited Drosha. Therefore, many laboratories have studied DGCR8 binding to RNA in the absence of Drosha and have shown that deletion constructs of DGCR8 can multimerize in the presence of RNA. More recently, it was demonstrated that Drosha can bind pri-miRNA substrates in the absence of DGCR8, casting doubt on the sequential model of binding. In the same study, using a single-molecule photobleaching assay, fluorescent protein-tagged deletion constructs of DGCR8 and Drosha assembled into a heterotrimeric complex on RNA, comprising two DGCR8 molecules and one Drosha molecule. To determine the stoichiometry of Drosha and DGCR8 within the MC in the absence of added RNA, we also used a single-molecule photobleaching assay and confirmed the heterotrimeric model of the human MC. We demonstrate that a heterotrimeric complex is likely preformed in the absence of RNA and exists even when full-length proteins are expressed and purified from human cells, and when hAGT-derived tags are used rather than fluorescent proteins.

Towards Digital Twins Cloud Platform: Microservices and Computational Workflows to Rule a Smart Factory

Abstract: The concept of "Industry 4.0" considers smart factories as data-driven and knowledge enabled enterprise intelligence. In such kind of factory, manufacturing processes and final products are accompanied by virtual models -- Digital Twins. To support Digital Twins concept, a simulation model for each process or system should be implemented as independent computational service. The only way to implement an orchestration of a set of independent services and provide scalability for simulation is to use a cloud computing platform as a provider of the computing infrastructure. In this paper, we describe a Digital Twin-as-a-Service (DTaaS) model for simulation and prediction of industrial processes using Digital Twins.

Compact approximations to the bottom friction term, for the study of tides propagating in channels

Abstract: Approximations to the bottom friction term Ku | u |/( H + Z ), found in the equations of tidal hydrodynamics, are presented, taking fully into account its nonlinear character. In upstream regions where the current no longer reverses, the friction term becomes 2 Ku 0 u 1 / H as a first approximation, u 0 being the steady current, u 1 the oscillatory contribution. A two-term approximation reproduces the quadratic variation of the current field in the friction term, in zones where the current reverses: this approximation suffices to predict and understand the peculiarities in the damping of the incoming tidal components, associated with quadratic friction. It also helps identify and evaluate the compound harmonics which result from their nonlinear frictional interactions. three-term approximation should be applied when numerical accuracy becomes necessary. The approximations described have already shown themselves most helpful in the understanding of the effects of quadratic friction in tidal phenomena.