Ensenada Center for Scientific Research and Higher Education

About: Ensenada Center for Scientific Research and Higher Education is a facility organization based out in Ensenada, Mexico. It is known for research contribution in the topics: Population & Nonlinear system. The organization has 1934 authors who have published 3733 publications receiving 63115 citations.

Neurospora crassa NKIN2, a Kinesin-3 Motor, Transports Early Endosomes and Is Required for Polarized Growth

Abstract: Biological motors are molecular nanomachines, which convert chemical energy into mechanical forces. The combination of mechanoenzymes with structural components, such as the cytoskeleton, enables eukaryotic cells to overcome entropy, generate molecular gradients, and establish polarity. Hyphae of filamentous fungi are among the most polarized cells, and polarity defects are most obvious. Here, we studied the role of the kinesin-3 motor, NKIN2, in Neurospora crassa. We found that NKIN2 localizes as fast-moving spots in the cytoplasm of mature hyphae. To test whether the spots represented early endosomes, the Rab5 GTPase YPT52 was used as an endosomal marker. NKIN2 colocalized with YPT52. Deletion of nkin2 caused strongly reduced endosomal movement. Combined, these results confirm the involvement of NKIN2 in early endosome transport. Introduction of a rigor mutation into NKIN2 labeled with green fluorescent protein (GFP) resulted in decoration of microtubules. Interestingly, NKIN2(rigor) was associated with a subpopulation of microtubules, as had been shown earlier for the Aspergillus nidulans orthologue UncA. Other kinesins did not show this specificity.

Stabilizing DFB laser injection-locked to an external fiber-optic ring resonator.

Abstract: Self-injection locking to an external fiber cavity is an efficient technique enabling drastic linewidth narrowing and self-stabilization of semiconductor lasers. The main drawback of this technique is its high sensitivity to fluctuations of the configuration parameters and surroundings. In the proposed laser configuration, to the best our knowledge, for the first time the self-injection locking mechanism is used in conjunction with a simple active optoelectronic feedback, ensuring stable mode-hopping free laser operation in a single longitudinal mode. Locking to 4-m length fiber resonator causes a drastic narrowing of the DFB laser linewidth down to 2.8 kHz and a reduction of the laser phase noise by three orders of magnitude. We have explored key features of the laser dynamics with and without active feedback, revealing stability and tunability of the laser linewidth as an additional benefit of the proposed technique.

Evaluation of the antibacterial activity of different species of phytoplankton

Abstract: The antibiotic activity of organic extracts from cultures of six species of phytoplankton, Synechococcus elongatus, Synechocystis sp., Amphiprora paludosa, Porphyridium cruentum, Chaetoceros muelleri and Dunaliella tertiolecta was examined. The bacteria used were Gram(-) strains, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae, and the Gram(+) strains were Bacillus subtilis, Staphylococcus aureus, and Microccocus luteus. All extracts inhibited the growth of B. subtilis and effected the generation of inhibition halos, ranging in diameter from 7.06 to 15.23 mm, for several bacteria species. Wide-spectrum antibacterial activity was observed in extracts from S. elongatus, Synechocystis sp., A. paludosa, P. cruentum, and C. muelleri. Thus, this five phytoplankton species can be used to control bacteria.

Photoprotection in the brown alga Macrocystis pyrifera: evolutionary implications.

Abstract: The dissipation of energy as heat is essential for photosynthetic organisms to protect themselves against excess light. We compared Photosystem II florescence changes (non-photochemical quenching, NPQ) in the brown alga Macrocystis pyrifera with that of Ficus sp., a higher plant to examine if the mechanism of heat dissipation (energy-dependent quenching, qE) differs between these evolutionary distant groups of phototrophs. We discovered that M. pyrifera had a slower rise of NPQ upon illumination than the Ficus sp. Further, the NPQ relaxation phase that takes place in the first minutes after light to dark transition is absent in this brown alga. We found that the NPQ induction rate in this alga was 1.5 times faster in preilluminated samples than in dark-adapted samples; this was associated with an increase in the rate of accumulation of the carotenoid zeaxanthin. Therefore, we conclude that NPQ in M. pyrifera is associated only with the formation of zeaxanthin. These results indicate that M. pyrifera lacks the fast component of qE that is related to allosteric changes in the light harvesting complexes of Ficus sp., a representative of higher plants. Although the xanthophyll cycle of this brown alga is similar to that of Ficus sp., yet, the transthylakoid proton gradient (ΔpH) does not influence NPQ beyond the activation of the violaxanthin de-epoxidase enzyme. These findings suggest that NPQ control mechanisms are not universal and we suggest that it may have diverged early in the evolution of different groups of eukaryotic phototrophs.

LaCasa: Location and context-aware safety assistant

Abstract: Wandering is a common behavior among people with dementia (PwD). It is also one of the main concerns of caregivers since it can cause the person to get lost and injured. The frequency and manner in which a person wanders is highly influenced by the person's background and contextual factors specific to the situation. In this paper we investigate some of the properties of wandering behaviours, particularly related to our ability to sense them with mobile devices. We then propose a novel decision-theoretic model that estimates the risk faced by the PwD and decides on the appropriate action to take, such as prompting the PwD or calling the caregiver. The model can be tailored to the user needs (e.g. known locations, level of cognitive decline) and takes into account uncertainty, and contextual information gathered from sensors, such as current location, noise, and proximity to the caregiver. A preliminary version of the system has been instantiated in a wandering assistance application for mobile devices running on an Android platform.