Showing papers by "Hyogo College of Medicine published in 1983"
TL;DR: Normal Japanese subjects were divided into two groups, i.e., one with both low and high Km isozymes of aldehyde dehydrogenase for acetaldehyde and the other deficient in the low Km isozyme, and the frequency of deficient phenotype of ALDH in Japanese was presumed to be about 36%.
Abstract: Normal Japanese subjects were divided into two groups, ie, one with both low and high Km isozymes of aldehyde dehydrogenase for acetaldehyde, and the other deficient in the low Km isozyme After intake of 04 g/kg alcohol, the deficient subjects showed high level of blood acetaldehyde, facial flushing and the other dysphoric symptoms, including increase of pulse rate, decrease of diastolic blood pressure, changes of pulse wave in the fingertip, and elevation of the arterial pressure and blood flow rate in common carotid arteries as well as increase of plasma catecholamines level In contrast, subjects with normal ALDH did not show these changes From the observation of liver specimens obtained at autopsy, the frequency of deficient phenotype of ALDH in Japanese was presumed to be about 36%
163 citations
TL;DR: The scalp distributions, differential experimental manipulations and developing time-courses suggest that the early negativity manifests the auditory discrimination activity on a task-relevant stimulus for deciding whether it is a target or not, whereas the late negativity reflects modality-nonspecific activation processes which may be associated with ‘momentary effort’ or ‘orienting’ triggered by detection of the task relevancy.
75 citations
TL;DR: A sensitive analytical method was developed in order to study the rhodopsin-porphyropsin system in the eye and the results agreed with those obtained from the bleaching kinetics of visual pigment extracted with detergent.
68 citations
TL;DR: Plasma FN levels were correlated significantly not to the degree of leptomeningeal proliferation but to the MAGS scores in gliomas, and deep immunoperoxidase staining for FN was shown in the endothelial cells and the psammoma bodies.
Abstract: The presence of fibronectin (FN) and glial fibrillary acidic protein (GFAP) in two astrocytomas, 17 glioblastomas, and five meningiomas was studied by indirect immunoproxidase staining of formalin-fixed and paraffin-embedded surgical specimens. Angiogenesis in tumor was scored by the microscopic angiogenesis grading system (MAGS), and plasma FN levels were measured by single radial immunodiffusion. In astrocytomas and glioblastomas, GFAP-positive tumor cells had no FN expression and FN was confined to proliferating vessel walls and the leptomeninges, showing a mutually exclusive FN and GFAP expression. GFAP-positive tumor cells were occasionally surrounded by a network of FN-positive matrix produced by cells derived from the leptomeninges or blood vessels. In meningiomas, FN expression was found in vessel walls and meningioma cells including whorl formations and psammoma bodies. In general, deep immunoperoxidase staining for FN was shown in the endothelial cells and the psammoma bodies. Plasma FN levels were correlated significantly not to the degree of leptomeningeal proliferation but to the MAGS scores in gliomas.
58 citations
TL;DR: In this paper, the authors examined mast-cell precursors in various in vivo and in vitro haematopoietic colonies of mice and found that more than 40% of 12 d exogenous spleen and 14 d in vitro mixed colonies contained mast cells.
Abstract: We have examined mast-cell precursors in various in vivo and in vitro haematopoietic colonies of mice. Cells from haematopoietic colonies of (WB x C57BL/6)F1- + +/+ mouse origin were injected into the skin of the congeneic W/Wv mice which are genetically depleted of tissue mast cells. The appearance of mast-cell clusters at the injection site indicated the presence of mast-cell precursors in the injected cell suspension. More than 40% of 12 d exogenous spleen colonies and 14 d in vitro mixed colonies contained mast-cell precursors, but only a small proportion of 7 d exogenous spleen colonies, 9 d in vitro erythroid bursts or 14 d large in vitro neutrophil-macrophage colonies contained mast-cell precursors. Neither transient endogenous erythroid spleen colonies examined at the fifth day nor 7 d in vitro neutrophil-macrophage colonies contained mast-cell precursors. Among 12 d spleen colonies mast-cell precursors were more frequent in the predominantly neutrophil than the predominantly erythroid colonies but 14 d in vitro mixed colonies, in which erythroid cells were predominant, contained mast-cell precursors more frequently than 14 d in vitro neutrophil-macrophage colonies. Therefore, differentiation of mast cells seems to be independent of either the neutrophil-macrophage or erythroid cell lineages.
35 citations
TL;DR: The dose-response relationship between low-caloric sweetening agents, sorbitol and maltitol, administered orally in single doses, and the induction of diarrheal symptoms was investigated and the maximum non-effective dose of Sorbitol was estimated to be 0.15 g/kg.
34 citations
TL;DR: The structure and location of the dorsal and ventral tegmental nuclei of Gudden were studied in brains of 12 species of mammals.
Abstract: The structure and location of the dorsal and ventral tegmental nuclei of Gudden were studied in brains of 12 species of mammals. The ventral teg-mental nucleus of Gudden is composed of oval or polygonal, medium-sized, well-stained cells, and includes the pars principalis situated ventral to the fasciculus longitudinalis medialis, and the pars suprafascicularis in the cen-tral gray matter dorsal to the fasciculus longitudinalis medialis (in the golden hamster, mouse, vole, and house shrew).
The dorsal tegmental nucleus of Gudden is situated within the central gray matter. This nucleus includes the pars ventralis, composed of oval or triangular cells, medium-sized but smaller than those of the ventral tegmen-tal nucleus of Gudden, and the pars dorsalis, composed of round, small, and lightly stained cells, and often encapsulated by fibers of the fasciculus longi-tudinalis dorsalis.
The isthmus between the dorsal and ventral tegmental nuclei of Gudden is distinct. In the cat, dog, rat, gerbil, and rabbit, the isthmus stands at the dorsal edge of the fasciculus longitudinalis medialis; in the golden hamster, mouse, vole, and house shrew, within the central gray matter; and in the guinea pig, at the ventral edge of the fasciculus longitudinalis medialis. In the green monkey and man, we could not find a ventral tegmental nucleus of Gudden, and in the house shrew and man, the pars ventralis and pars dor-salis of the dorsal tegmental nucleus of Gudden could not be separated.
33 citations
TL;DR: Histological examination revealed that both US and EXP units are NA motoneurons responsible for ultrasound emission, suggesting that both UK-related and UK-based units are ultrasound-related motoneurs responsible for ultrasounds.
Abstract: Unit discharges associated with ultrasonic vocalization were recorded from the rat medullary region including the nucleus ambiguous (NA). Three types of units were distinguished: ultrasound-related (US), expiration (EXP) and inspiration (INSP) units. Both US and EXP units discharged with tonic bursts mostly prior to ultrasounds. US units were silent during respiration while EXP units discharged synchronously with expiration. During ultrasonic vocalization INSP units suppressed their discharges which were otherwise synchronized with inspiration. Histological examination revealed that US and EXP units were recorded from the ventral division of the NA and the adjacent medullary region where the NA contained large cells and the adjacent region consisted of small cells. These results suggest that both US and EXP units are NA motoneurons responsible for ultrasound emission. EXP units were always supplied with respiratory afferents while US units were not.
32 citations
TL;DR: Spinasterol inhibits cholesterol absorption, resulting in decreases of the plasma and liver cholesterol levels and that when cholesterol absorption is inhibited, the synthesis of bile acids, especially that of chenodeoxycholic acid, decreases, suggesting that the dietary cholesterol is preferentially metabolized to chenodesoxycholics acid in mice.
25 citations
TL;DR: Visual pigment composition was determined by HPLC analysis over a one-year period in the adult bullfrog (Rana catesbeiana) in Japan and in Japan, the vitamin A2-based pigment increased in November and reached a maximum of 32-36% between January and June.
24 citations
TL;DR: The present ultrastructural, finding is unusual in the neurofibrillary pathology of PSP, and is also different from the PHF found in Alzheimer type NFD.
Abstract: Unusual paired helical filaments (PHF) coexisting with single filaments were observed in neurofibrillary degeneration (NFD) in the globus pallidus, subthalamic nucleus, substantia nigra, and pontine tegmentum of a typical case of progressive supra-nuclear palsy (PSP). Each filament had a diameter of 10-12 nm and showed central low density and a smooth contour. The thickest portion of a pair was 22-24 nm in diameter. The periodicity of twist varied from 150 nm to 300 nm, but each PHF had regular periodicity. The present ultrastructural finding is unusual in the neurofibrillary pathology of PSP, and is also different from the PHF found in Alzheimer type NFD.
TL;DR: Maltitol or sorbitol was administered in doses of 0.8 g/kg to 20 physically healthy subjects and 6 diabetic patients and caused diarrhea in 75% and 95% of the subjects, respectively.
TL;DR: The case herein described was a patient with mesonephric adenocarcinoma, confirmed by autopsy to originate from the bladder, and seems to be only the third case of such tumor in the literature.
Abstract: Mesonephric adenocarcinoma of the urinary bladder is an extremely rare tumor and appears to arise from the embryonic cell rests in the bladder The case herein described was a patient with mesonephric adenocarcinoma, confirmed by autopsy to originate from the bladder, and with this paper, seems to be only the third case of such tumor in the literature
TL;DR: Results show that the 5 alpha-reduct enzyme activity is regulated by LH, while the 17 beta-ol-dehydrogenase activity is stimulated by FSH in immature golden hamster ovary, but the effect of FSH is largely mediated by estrogen.
TL;DR: In the diagnosis of annular pancreas, CT provided unequivocal evidence before operation and succeeded in visualization of a peninsular protrusion of the parenchyma from the pancreatic head.
Abstract: A patient with annular pancreas is described. CT succeeded in visualization of a peninsular protrusion of the parenchyma from the pancreatic head. In the diagnosis of annular pancreas, CT provided unequivocal evidence before operation.
TL;DR: Motoneurons in the nucleus ambiguus for the superior and the recurrent laryngeal nerves were identified by antidromic stimulation of these nerves in rats anesthesized with urethane in good agreement with previous morphological studies.
TL;DR: This C6 treatment increased the sensitivity of the contractile response of the intestine to muscarinic agonists, possibly by increasing mACh-R.
TL;DR: CT was valuable for diagnosis of the small hepatocellular carcinomas larger than 1 cm by using the dynamic study after an intravenous bolus injection of the contrast medium.
Abstract: Thirty-two patients with hepatocellular carcinoma less than 5 cm in diameter were examined by computed tomography. At the examination 26 patients (81%) were correctly diagnosed. A common type of CT image series was detectable as low density on the precontrast scan, a positive or mixed pattern on dynamic scan, and visible on postcontrast scan. In 10 patients with minute tumors (less than 2 cm), 7 were correctly diagnosed. CT was valuable for diagnosis of the small hepatocellular carcinomas larger than 1 cm by using the dynamic study after an intravenous bolus injection of the contrast medium.
TL;DR: The results may indicate that some CFU-MIX proliferate and differentiate along B-cell lineage to sIGM or cIgM bearing cells in vitro.
Abstract: Recent progress in clonal cell culture techniques makes it possible to detect pluripotent hemopoietic precursors from murine marrow cells. The precursors can proliferate, differentiate and form mixed colonies containing eryth-roblasts, granulocytes, macrophages and often megakaryocytes in viscid culture medium. In the present investigation, the presence of cells of B-cell lineage in mixed colonies was investigated.
Experiments on colonies containing clgM, clgG, slgM and slgG bearing cells using goat IgG fluorescein-conjugated anti-mouse IgM, goat F(ab′)2 fraction fluo-rescein-conjugated anti-mouse IgG and immunobeads revealed the presence of cytoplasmic IgM bearing cells in 47% of the colonies and surface IgM bearing cells in 74-84% of the colonies. Mixed colonies, however, did not contain either clgG bearing cells or slgG bearing cells. The results may indicate that some CFU-MIX proliferate and differentiate along B-cell lineage to slgM or clgM bearing cells in vitro.
TL;DR: The length of time between the ingestion of test substances and the first onset of diarrheal stool varied widely from less than one hour to 23 hours among individuals, however, adzuki-bean jelly diarrhea was induced within 5 hours in all subjects.
TL;DR: High baseline levels of sister chromatid exchanges in peripheral blood lymphocytes of BS were reduced to about 10% of their initial value in BS lymphoblastoid cell lines, and the elevation of SCE frequencies induced by ethylmethanesulfonate was the same in controls.
Abstract: Seven lymphoblastoid cell lines were established through transformation by Epstein-Barr virus of peripheral blood lymphocytes from two patients with Bloom's syndrome (BS), the parents of a patient, and normal controls. High baseline levels of sister chromatid exchanges (SCEs) in peripheral blood lymphocytes of BS were reduced to about 10% of their initial value in BS lymphoblastoid cell lines, and the elevation of SCE frequencies induced by ethylmethanesulfonate was the same as in controls.
TL;DR: An increase in oxidative catabolism of medium nutrients in B cells grown in medium with 2-deoxy-D-glucose for 7 days is suggested, and such metabolic changes may promote the growth of B cells in vitro.
Abstract: The culture for 7days in medium with 5.5mM glucose and 1mM 2-deoxy-Dglucose enhanced the glucose sensitivity of neonatal rat B cells, and even stimulated their growth in vitro. Also, 2-deoxy-D-glucose supplementation maintained insulin release evoked by leucine and 2-ketoisocaproate from B cells at day 7 at levels several times higher than at day 1. The effect of leucine was greatly augmented by glutamine, whereas that of the 2-keto acid remained almost unchanged irrespective of the presence of glutamine. These results suggest an increase in oxidative catabolism of medium nutrients in B cells grown in medium with 2-deoxy-D-glucose for 7days, and such metabolic changes may promote the growth of B cells in vitro.
TL;DR: This is the first report of a patient for whom a successful radical treatment for both membranous obstruction of the inferior vena cava (MOVC) and coincident hepatocellular carcinoma (HCC) were simultaneously carried out.
Abstract: To our knowledge, this is the first report of a patient for whom a successful radical treatment for both membranous obstruction of the inferior vena cava (MOVC) and coincident hepatocellular carcinoma (HCC) were simultaneously carried out. A 36 year old Japanese man with Budd-Chiari syndrome due to MOVC was found to have HCC in the right hepatic lobe during a shunt operation between the inferior vena cava (IVC) and the right atrium (RA), using a vascular prosthetic graft. He was referred to us from another institute for further operative procedures. Both the mediastinum and the abdomen were entered through a long midline incision with longitudinal splitting of the sternum. The graft that had been occluded by thrombus was removed. Transcardiac membranotomy was achieved by manual manipulation and then by Tubb's dilator. The hepatic tumor with an abundant surgical margin of the liver was resected. The tumor of 2.5×2.5×2.0 cm was a well differentiated clear cell type HCC associated with congestive liver cirrhosis. Postoperative contrast and pressure studies through IVC showed satisfactory results. Serum alfa-fetoprotein levels decreased from 503 to 12 ng/ml. The patient is well at least for 44 months after the surgery without any recurrence at the time of completion of this writing.
TL;DR: Findings suggest an important role of the duodenum in the entero-PP axis in man, and in BI patients with pyloric ring preservation the PP response to a meal was almost normal.
TL;DR: It was considered that induction of suppressor cells might be due to depressed function of the epidermal Langerhans cell affected by potent sensitizers as well as by unknown mechanisms.
Abstract: From the present experiments in which sensitization was induced by painting contactants on the same skin of the same mouse over time, the following findings were observed.
When mice were sensitized first with PCl, then with DNFB, and finally with OX, the animals showed a normal contact hypersensitivity response to PCl, but abnormal responses to both DNFB and OX, suggesting some suppressor phenomenon.
This suppression was shown to be partly due to hapten (DNFB)-specific suppressor cells, and it was suggested that other suppressor cells different from suppressor T cells in nature might be involved.
It was considered that induction of suppressor cells might be due to depressed function of the epidermal Langerhans cell affected by potent sensitizers as well as by unknown mechanisms.
TL;DR: The thyroiditis was mycotic and developed severe necrotic inflammation with a hemorrhage and a perithyroidal abscess, and resulting in disseminating mycosis with multiple microabscesses in both kidneys and in myocardium.
Abstract: An autopsy case of acute thyroiditis in acute lymphocytic leukemia is presented. The thyroiditis was mycotic and developed severe necrotic inflammation with a hemorrhage and a perithyroidal abscess, and resulting in disseminating mycosis with multiple microabscesses in both kidneys and in myocardium.
TL;DR: The columnar villi at the early postnatal stage grew wider and fused gradually with each other accompanying the advance of age and formed net-arranged folds in the duodenum and the wavy fold in the jejunum and ileum at the adult stage.