Institution
Institute of Medical Sciences, Banaras Hindu University
Education•Varanasi, India•
About: Institute of Medical Sciences, Banaras Hindu University is a education organization based out in Varanasi, India. It is known for research contribution in the topics: Population & Visceral leishmaniasis. The organization has 3622 authors who have published 4579 publications receiving 84718 citations.
Papers published on a yearly basis
Papers
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TL;DR: Monastrol is confirmed as a potent inhibitor of PTR1 in Leishmania; it inhibits proliferation of amastigotes with an IC(50) (50% inhibitory concentration) of 10 microM in macrophage cultures infected with an L. donovani clinical isolate, with no host cytotoxicity, giving therapeutic backing to the use of monastrol as an oral antileishmanial in human VL cases.
Abstract: Methods: Monastrol (R) and (S) enantiomers were docked using the QUANTUM program into the active site of a Leishmania donovani PTR1 (LdPTR1) homology model. A structure‐activity relationship based on a homology model of a recombinant enzyme was substantiated by a recombinant enzyme inhibition assay. We adapted an L. donovani (transfected with green fluorescent protein) intramacrophage amastigote screening assay as a cellular model for leishmaniasis. Furthermore, since the clinicopathological features and immunopathological mechanisms of visceral leishmaniasis (VL) in a hamster model are remarkably similar to those of human disease, systemic infection of hamsters with L. donovani was utilized to collect in vivo data for monastrol. Results: Both monastrol (R) and (S) enantiomers fit well in the ligand-binding pocket of LdPTR1. Monastrol exhibits a Ki value of 0.428 mM in the recombinant enzyme inhibition assay. We confirm monastrol as a potent inhibitor of PTR1 in Leishmania; it inhibits proliferation of amastigotes with an IC50 (50% inhibitory concentration) of 10 mM in macrophage cultures infected with an L. donovani clinical isolate, with no host cytotoxicity. We also show that in experimental animals, oral administration of a 5 mg/kg dose of monastrol on two alternate days inhibits 50% of parasite growth, giving therapeutic backing to the use of monastrol as a potent antileishmanial in human VL cases.
36 citations
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TL;DR: KMP-11 is a unique antigen with high epitope density that activates CD4(+) and CD8(+) T-cell mediated immunity to confer resistance to VL and could provide a potent strategy for future anti-leishmanial vaccine development.
36 citations
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TL;DR: Children with advanced arthritis and healed infection should be informed and discussed the various treatment modalities including the joint replacement, and surgeons are taking up the challenge of putting the total hip replacement in the active stage of the disease.
Abstract: Tuberculosis (TB) of the hip is second to spine only hence a good number of cases are visiting the medical facilities every year. Many present in the advanced stage of the disease due to delayed diagnosis. In early stages of TB of hip, there is a diagnostic dilemma when plain X-rays are negative. In the present time, diagnostic modalities have improved from the days when diagnosis was based essentially on clinicoradiological presentation alone. By the time definite radiological changes appear on plain X-ray, the disease has moderately advanced. The modern diagnostic facilities like ultrasonography (USG) or magnetic resonance imaging of the hip joint, USG guided aspiration of synovial fluid and obtaining the material for polymerase chain reaction and tissue diagnosis must be utilized. In the treatment, current emphasis is more on mobility with stability at hip. Joint debridement, skeletal traction, and mobilization exercises may give more satisfying results as compared to the immobilization by hip spica. Adults with advanced arthritis and healed infection should be informed and discussed the various treatment modalities including the joint replacement. More and more surgeons are taking up the challenge of putting the total hip replacement in the active stage of the disease. Until the long term results in active disease are well established, we recommend it for the healed disease only in selected cases.
36 citations
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TL;DR: Five cases of urethral diverticulum in male subjects are presented, of which 2 were congenital and 3 were acquired, and one congenital diverticula contained multiple calculi.
36 citations
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TL;DR: The combination of one intravenous administration of 5mg/kg Ambisome and oral administration of miltefosine, 2.5mg/ kg/day for 14 days, was evaluated in 135 Indian patients with kala-azar, finding this combination is attractive for reasons of efficacy, tolerance, and feasibility of administration, although the gastrointestinal side effects of MiltefOSine require medical vigilance.
Abstract: The combination of one intravenous administration of 5mg/kg Ambisome and oral administration of miltefosine, 2.5mg/kg/day for 14 days, was evaluated in 135 Indian patients with kala-azar. The Intent-to-Treat cure rate at 6 months was 124 of the 135 enrolled patients (91.9%: 95% CI = 86-96%), and the per protocol cure rate was 124 of 127 evaluable patients (97.6%: 95% CI = 93-100%). Side effects could be attributed to each drug separately: fevers, rigors and back pain due to Ambisome; gastrointestinal side effects due to miltefosine. This combination is attractive for reasons of efficacy, tolerance, and feasibility of administration, although the gastrointestinal side effects of miltefosine require medical vigilance. Clinical Trials.gov identification number: NCT00371995.
36 citations
Authors
Showing all 3679 results
Name | H-index | Papers | Citations |
---|---|---|---|
A. Kumar | 96 | 505 | 33973 |
Sandeep Kumar | 94 | 1563 | 38652 |
Shyam Sundar | 86 | 614 | 30289 |
Pramod K. Srivastava | 79 | 390 | 27330 |
Rajesh Gupta | 78 | 936 | 24158 |
Naresh Kumar | 66 | 1106 | 20786 |
Marleen Boelaert | 64 | 386 | 16328 |
Srinivasa M. Srinivasula | 63 | 98 | 32847 |
Amit Singh | 57 | 640 | 13795 |
Rakesh K. Singh | 56 | 335 | 12617 |
Surya Prakash Singh | 55 | 736 | 12989 |
Hari Shanker Sharma | 51 | 252 | 8366 |
Jai Prakash | 51 | 259 | 8243 |
Vijay K. Singh | 45 | 467 | 7792 |
Madhu Dikshit | 43 | 210 | 5327 |