Institution
Jimei University
Education•Amoy, China•
About: Jimei University is a education organization based out in Amoy, China. It is known for research contribution in the topics: Computer science & Population. The organization has 4645 authors who have published 4293 publications receiving 42239 citations. The organization is also known as: Jíměi Dàxué.
Topics: Computer science, Population, Larimichthys crocea, Gene, Chemistry
Papers published on a yearly basis
Papers
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TL;DR: In this paper, a 2D numerical wave tank is built in which the simulations are conducted using computational fluid dynamics (CFD) approach, while a strong-coupled fluid-structure interaction (FSI) algorithm is applied to consider the hydroelastic effects.
19 citations
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TL;DR: The principle of substitution is used to replace sub-gaskets of the Sierpinski gasket network by an equivalent Y-network which enables the use of only the Delta–Wye transformation and the series and parallel principles to derive some two-point resistances of the H2O network with dimension two.
Abstract: In this paper, we use the principle of substitution to replace sub-gaskets of the Sierpinski gasket network by an equivalent Y-network which enables the use of only the Delta–Wye transformation and the series and parallel principles to derive some two-point resistances of the Sierpinski gasket network with dimension two.
19 citations
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TL;DR: Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish snakehead by ammonium sulfate fractionation, anion exchange, and gel filtration and exhibited different immunological reactions.
Abstract: Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish snakehead (Channa argus) by ammonium sulfate fractionation, anion exchange, and gel filtration. Two-dimensional gel electrophoresis and native-PAGE analysis revealed that their molecular masses were 37, 38, and 36 kDa and their isoelectric points 4.8, 4.4, 4.0, respectively. All of the pepsinogens converted into their active form pepsins under pH 2.0 by one-step pathway or stepwise pathway. The three pepsins showed maximal activity at pH 3.0, 3.5, and 3.0 with optimum temperature at 45, 40, and 40 °C, respectively, using hemoglobin as substrate. All of the pepsins were completely inhibited by pepstatin A, a typical aspartic proteinase inhibitor. The N-terminal amino acid sequences of the three pepsinogens were determined to the 34th, 25th, and 28th amino acid residues, respectively. Western blot analysis of the three PGs exhibited different immunological reactions.
19 citations
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TL;DR: A modified SSF system was established to address the shortcomings of the traditional solid-state fermentation (SSF) system, and tannase production through the traditional tea stalk SSF was markedly improved when the optimized parameters were applied.
Abstract: High amounts of insoluble substrates exist in the traditional solid-state fermentation (SSF) system. The presence of these substrates complicates the determination of microbial biomass. Thus, enzyme activity is used as the sole index for the optimization of the traditional SSF system, and the relationship between microbial growth and enzyme synthesis is always ignored. This study was conducted to address this deficiency. All soluble nutrients from tea stalk were extracted using water. The aqueous extract was then mixed with polyurethane sponge to establish a modified SSF system, which was then used to conduct tannase production. With this system, biomass, enzyme activity, and enzyme productivity could be measured rationally and accurately. Thus, the association between biomass and enzyme activity could be easily identified, and the shortcomings of traditional SSF could be addressed. Different carbon and nitrogen sources exerted different effects on microbial growth and enzyme production. Single-factor experiments showed that glucose and yeast extract greatly improved microbial biomass accumulation and that tannin and (NH4)2SO4 efficiently promoted enzyme productivity. Then, these four factors were optimized through response surface methodology. Tannase activity reached 19.22 U/gds when the added amounts of tannin, glucose, (NH4)2SO4, and yeast extract were 7.49, 8.11, 9.26, and 2.25%, respectively. Tannase activity under the optimized process conditions was 6.36 times higher than that under the initial process conditions. The optimized parameters were directly applied to the traditional tea stalk SSF system. Tannase activity reached 245 U/gds, which is 2.9 times higher than our previously reported value. In this study, a modified SSF system was established to address the shortcomings of the traditional SSF system. Analysis revealed that enzymatic activity and microbial biomass are closely related, and different carbon and nitrogen sources have different effects on microbial growth and enzyme production. The maximal tannase activity was obtained under the optimal combination of nutrient sources that enhances cell growth and tannase accumulation. Moreover, tannase production through the traditional tea stalk SSF was markedly improved when the optimized parameters were applied. This work provides an innovative approach to bioproduction research through SSF.
19 citations
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TL;DR: RT-PCR analysis showed that LycRan expression was obviously up-regulated in kidney, blood and spleen after immunization with either poly I:C or formalin-inactive Gram-negative bacterium Vibrio parahaemolyticus, suggesting that LyCRan might play an important role in large yellow croaker defense against the pathogen infection.
19 citations
Authors
Showing all 4696 results
Name | H-index | Papers | Citations |
---|---|---|---|
Feng Wang | 107 | 1136 | 64644 |
Feng Chen | 95 | 2138 | 53881 |
Jing Feng | 54 | 476 | 11869 |
Duong D. Do | 51 | 558 | 15365 |
Yanqiang Huang | 49 | 142 | 8194 |
Quansheng Chen | 48 | 304 | 7583 |
Qingbiao Li | 43 | 254 | 7881 |
Fuwen Yang | 41 | 201 | 7371 |
Pin Nie | 40 | 226 | 5484 |
David Machin | 36 | 108 | 5647 |
Lin Xu | 32 | 72 | 3521 |
Xiaomei Chen | 32 | 76 | 3762 |
Tao Wu | 32 | 154 | 3007 |
Min-Jie Cao | 30 | 166 | 3056 |
Fuhang Song | 29 | 87 | 1941 |