Jimei University

About: Jimei University is a education organization based out in Amoy, China. It is known for research contribution in the topics: Computer science & Population. The organization has 4645 authors who have published 4293 publications receiving 42239 citations. The organization is also known as: Jíměi Dàxué.

Apoptosis induced by acrylamide is suppressed in a 21.5% fat diet through caspase-3-independent pathway in mice testis.

Abstract: This study investigates the simultaneous effect of acrylamide (ACR) and high-fat-intake on the apoptosis in testis cells, and also the expression and activity of caspase-3. Seventy-two male Kunming mice were divided into two blocks and fed with a high-fat diet (crude fat 21.5%) or basic diet (crude fat 4.4%), respectively; and animals in each diet block were exposed to ACR at the dose of 20 mg/kgbw•d or 40 mg/kgbw•d as ACR treated groups or the normal saline as control. Germ cells prepared from testis were stained with Hoechst dye 33258 and paraffin wax sections from testis were suffered to a TUNEL process. Expression of caspase-3 on protein level was investigated using an immunohistochemical analysis assay. The supernatant of unilateral testes were subjected to a Caspase-3 activity kit to determine the activity of Caspase-3 in testis. The concentration of ACR and glycidamide(GA), epox-ide of ACR, in plasma and testis were detected by LC-ES/MS/MS analysis. Results based on the morphological changes, percentage of apoptotic cells, and integrated optical density (IOD) of positive amethyst staining which indicates the apoptotic DNA fragmentation, show that apoptosis was induced by acrylamide only; however, acr-ylamide-induced apoptosis was weakened by high-fat-intake. The protein expression and activity of Caspase-3 were not induced by ACR or high-fat-intake. Moreover, no significant differences of ACR and GA concentration were found between the high-fat and basic diet groups after exposure of ACR. Results indicate that high-fat-intake reverses the effects on apoptosis induced by ACR; and more possibly, apoptosis is induced by a caspase-3-independent mechanism.

Prior knowledge-based deep learning method for indoor object recognition and application

Abstract: Indoor object recognition is a key task for indoor navigation by mobile robots. Although previous work has produced impressive results in recognizing known and familiar objects, the research of ind...

Biochemical characterization of trypsins from the hepatopancreas of Japanese sea bass (Lateolabrax japonicus).

Abstract: A cationic trypsin (trypsin A) and an anionic trypsin (trypsin B) were highly purified from the hepatopancreas of the Japanese sea bass ( Lateolabrax japonicus ) by ammonium sulfate precipitation, column chromatographies of DEAE-Sepharose and Sephacryl S-200 HR. Purified trypsins revealed single band on SDS-PAGE and their molecular masses were 21 kDa and 21.5 kDa, respectively. Trypsins A and B exhibited maximal activity at 40 °C, and shared the same optimal pH at 9.0 using Boc-Phe-Ser-Arg-MCA as the substrate. The two trypsins were stable up to 45 °C and in the pH range from 7.0 to 11.0. Trypsin inhibitors such as Pefabloc SC, PMSF and benzamidine are effective to these two enzymes and their susceptibilities were similar. Apparent K m s of trypsins A and B were 1.12 and 0.7 μM and k cat s of them were 72.08 and 67.79 S − 1 for Boc-Phe-Ser-Arg-MCA, respectively. The N-terminal amino acid sequences of the two trypsins were determined to the 24th residues, which were highly identical to trypsins from other species of fish while trypsins A and B only shared 45.8% identity. The digestive effect of the two trypsins on native shrimp muscular proteins indicated their effectiveness in the degradation of food proteins.

Study on the ecological safety of algacides: a comprehensive strategy for their screening

Abstract: The ecological safety of 14 algacidal materials were appraised using algal growth inhibition tests as well as toxicity test for zooplankton and fish and prawn juveniles. In addition, an integrative analysis for evaluating their potential as algacides was made by comparing the 24-h LC50 and ratio of efficiency to safety (RES) of each material. The results showed that the growth of algae tested were not affected after 96 h of exposure to diallyl trisulfide even when the concentration was 10 mg L−1; there was a similar toxicity between garlic solution and diallyl trisulfide on seven species of aquatic organisms tested. Moreover, both prawn juveniles had lower resistances to garlic solution and diallyl trisulfide than the other 12 materials. According to the RES, green tea, garlic solution, diallyl trisulfide, and the Chinese medical herbs Cortex Fraxini, Herba Houttuyniae, Semen Arecae, and Rhizoma Coptidis are better algacides than the other materials tested in this study.

Air Annealing Effect on Oxygen Vacancy Defects in Al-doped ZnO Films Grown by High-Speed Atmospheric Atomic Layer Deposition.

Abstract: In this study, aluminum-doped zinc oxide (Al:ZnO) thin films were grown by high-speed atmospheric atomic layer deposition (AALD), and the effects of air annealing on film properties are investigated. The experimental results show that the thermal annealing can significantly reduce the amount of oxygen vacancies defects as evidenced by X-ray photoelectron spectroscopy spectra due to the in-diffusion of oxygen from air to the films. As shown by X-ray diffraction, the annealing repairs the crystalline structure and releases the stress. The absorption coefficient of the films increases with the annealing temperature due to the increased density. The annealing temperature reaching 600 °C leads to relatively significant changes in grain size and band gap. From the results of band gap and Hall-effect measurements, the annealing temperature lower than 600 °C reduces the oxygen vacancies defects acting as shallow donors, while it is suspected that the annealing temperature higher than 600 °C can further remove the oxygen defects introduced mid-gap states.