Jimei University

About: Jimei University is a education organization based out in Amoy, China. It is known for research contribution in the topics: Computer science & Population. The organization has 4645 authors who have published 4293 publications receiving 42239 citations. The organization is also known as: Jíměi Dàxué.

Portal of entry for pathogenic Vibrio alginolyticus into large yellow croaker Pseudosciaena crocea, and characteristics of bacterial adhesion to mucus.

Abstract: The portal of entry for pathogenic Vibrio alginolyticus into large yellow croaker Pseudosciaena crocea is via the intestinal tract rather than gill or skin according to the kinetics of the bacterial adhesion to different mucus. The different effects on adhesion caused by proteolytic enzymes and heat treatment might be due to the different chemical compositions of mucus. Adhesion of V. alginolyticus to mucus depends on concerted action of bacterial surface structures such as cell-surface proteins, somatic antigens, flagella, etc. In addition, starvation and monosaccharides, especially fructose, inhibit the bacterial adhesion to the mucus. Knowledge of these adhesive characteristics should be very useful for designing more efficacious prophylactic strategies.

Effect of dietary administration of Psychrobacter sp. on the growth, feed utilization, digestive enzymes and immune responses of grouper Epinephelus coioides

Abstract: A 60-day feeding trial was conducted to assess the effect of incorporating dietary Psychrobacter sp., one predominant bacterium with antagonistic activity in the gut of fast-growing grouper Epinephelus coioides, on the growth, feed utilization, digestive enzymes and immune responses of E. coioides at regular monthly intervals. The fish were fed with control diet (without probiont) or probiotic diet supplemented with 1.0 × 108 CFU g−1Psychrobacter sp. No significant improvement in weight gain (WG) and specific growth ratio (SGR) were observed in the probiotic group, but a significant improvement in feed conversion ratio (FCR) was observed in fish fed the probiotic diet for 60 days. Although not statistically significant, enhanced hepatopancreatic protease and lipase activities and intestinal amylase activity were observed in probiotic group at day 60. A slightly increase in phagocytic activity and phagocytic index was observed in fish fed probiotic diet for 60 days. Serum lysozyme activity showed no significant differences between the probiotic group and the control group during the whole experiment period, while an increasing trend of superoxide dismutase (SOD) activity in the probiotic group was observed. Serum complement component 3 (C3) level of the probiotic group was higher than that of control in the whole feeding period, while complement component 4 (C4) level of the probiotic group was significantly higher than that of the control at day 60. The results of the current study firstly demonstrated potential for Psychrobacter sp. as a novel probiont to improve feed utilization, digestive enzymes and innate immunity in E. coioides.

Rapid determination of malachite green in water and fish using a fluorescent probe based on CdTe quantum dots coated with molecularly imprinted polymer

Abstract: A fluorescent probe based on CdTe quantum dots (QDs) coated with molecularly imprinted polymers (MIPs) was fabricated for the determination of malachite green (MG) by the strategy of fluorescence resonance energy transfer (FRET). The MIP-coated QDs were synthesized through precipitation polymerization using acrylamide and ethylene glycol dimethacrylate as monomer and cross-linker, respectively. The optimum molar ratio of MG, monomer and cross-linker was 1:8:40 in 60 mL of acetonitrile, and the polymerization time was 28 h. The MIP-coated QDs, with an average diameter around 290 nm, showed excellent fluorescence emission in 500–700 nm at λ ex 370 nm. The probe exhibited fluorescence quenching response to MG selectively within only 5 min at the concentrations from 0.1 to 20 μmol L −1 . The fluorescent probe was successfully used to detect MG in water and fish samples with a detection limit of 0.059 μmol L −1 (3 σ , n = 9). The spiked recoveries, from 94.8% to 98.1% for water samples, and 98.1% to 106.2% for fish samples, respectively, indicated that the as-prepared MIP-coated QDs could be used as a fluorescent probe to detect MG rapidly in water and fish samples.