Korea Institute of Science and Technology Information

About: Korea Institute of Science and Technology Information is a facility organization based out in Daejeon, South Korea. It is known for research contribution in the topics: Gravitational wave & LIGO. The organization has 1152 authors who have published 2319 publications receiving 93849 citations. The organization is also known as: Korea Institute of Science and Technology Information & KISTI.

ΔNp63 intronic miR-944 is implicated in the ΔNp63-mediated induction of epidermal differentiation

Abstract: ΔNp63 is required for both the proliferation and differentiation of keratinocytes, but its role in the differentiation of these cells is poorly understood. The corresponding gene, TP63, harbors the MIR944 sequence within its intron. However, the mechanism of biogenesis and the function of miR-944 are unknown. We found that miR-944 is highly expressed in keratinocytes, in a manner that is concordant with that of ΔNp63 mRNA, but the regulation of miR-944 expression under various conditions did not correspond with that of ΔNp63. Bioinformatics analysis and functional studies demonstrated that MIR944 has its own promoter. We demonstrate here that MIR944 is a target of ΔNp63. Promoter analysis revealed that the activity of the MIR944 promoter was markedly enhanced by the binding of ΔNp63, which was maintained by the supportive action of AP-2 during keratinocyte differentiation. Our results indicated that miR-944 biogenesis is dependent on ΔNp63 protein, even though it is generated from ΔNp63 mRNA-independent transcripts. We also demonstrated that miR-944 induces keratin 1 and keratin 10 expression by inhibiting ERK signaling and upregulating p53 expression. Our findings suggested that miR-944, as an intronic miRNA and a direct target of ΔNp63, contributes to the function of ΔNp63 in the induction of epidermal differentiation.

An experiment and FE simulation for the development of a SPFC1180 AHSS one-body door impact beam about a car side collision

Abstract: This paper presents a new one-body door impact beam that was developed to protect an occupant when a car is involved in a side collision. SPFC1180 Advanced High Strength Steel (AHSS) was used to improve the impact energy absorbability and the small door dent to ensure safety inside of the car. To obtain the proper closed cross section shape of the door impact beam, several designs with the same inside area were considered by conducting a finite element (FE) bending analysis using the ANSYS Mechanical software. FE door modeling was validated with the impact simulation package of the LS-DYNA software, and the results were compared to those from the door module test. The validated door beam model was also subjected to a side collision test of a complete vehicle following the FMVSS 214 regulation using a parallel computing simulation, and a comparison was carried out for the pipe-typed door beam using a virtual structural test applicability. The results indicate that the SPFC1180 one-body door impact beam can exhibit an equivalent reaction force and can decrease manufacturing costs and weight when compared to a pipe typed door beam as a result of the AHSS material, simple manufacturing process, and one-body closed-section shape design.

Measurement of the inclusive semileptonic branching fraction B(Bs0→X-ℓ+νℓ) at Belle

Abstract: We report a measurement of the inclusive semileptonic B0s branching fraction in a 121 fb⁻¹ data sample collected near the Υ(5S) resonance with the Belle detector at the KEKB asymmetric energy e⁺e⁻ collider. Events containing B⁰(*)sB¯¯¯⁰(*)s pairs are selected by reconstructing a tag side D⁺s and identifying a signal side lepton l⁺ (l=e, μ) that is required to have the same-sign charge to ensure that both originate from different B⁰s mesons. The B⁰s→X⁻l⁺νl branching fraction is extracted from the ratio of the measured yields of D⁺s mesons and D⁺sl⁺ pairs and the known production and branching fractions. The inclusive semileptonic branching fraction is measured to be [10.6±0.5(stat)±0.7(syst)]%.

Bioworks: A Workflow System for Automation of Bioinformatics Analysis Processes

Abstract: Over the past decade, the rapid development of high-throughput technologies has lead to an explosive growth in biological information. Many molecular biology fields involve in-silico experiments using bioinformatics applications. A workflow can be well-defined model for bioinformatics analysis processes to be performed as a chain of interlinked data process tasks. We have developed a client/server-based workflow system called Bioworks, supporting large-scale analysis through the high performance cluster computing resources. In this paper, we highlight implementation methods in Bioworks, for meeting key requirements of effective workflow systems for bioinformatics analysis processes.