Institution
Pharmaceutical Product Development
Company•Wilmington, North Carolina, United States•
About: Pharmaceutical Product Development is a company organization based out in Wilmington, North Carolina, United States. It is known for research contribution in the topics: Immunotoxin & Fusion protein. The organization has 402 authors who have published 353 publications receiving 16396 citations.
Topics: Immunotoxin, Fusion protein, Population, Cancer, Antigen
Papers published on a yearly basis
Papers
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02 May 2019TL;DR: Professor of Pharmacology, People University of Medical & Health Sciences for Women, Pakistan.
Abstract: Luisetto M1*, Farhan Ahmad Khan2, Ahmed Yesvi Rafa3, Behzad Nili Ahmadabadi4 and Ghulam Rasool Mashori5 1Department of Applied pharmacologist, Independent Researcher, Italy 2Department of Pharmacology, Government Medical College, India 3Yugen Research Organization (YRO), Independent Researcher, Bangladesh 4Pharm D/PhD innovative Pharmaceutical product development specialist, USA 5Professor of Pharmacology, People University of Medical & Health Sciences for Women, Pakistan
3 citations
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TL;DR: A new method to visualize direct antibody-cell membrane interactions by surface plasmon resonance using the Biacore 3000 and on-line signal subtraction on antigen-negative cell membrane vesicles is developed.
Abstract: Due to problems of immobilizing functional tumor antigens in their natural conformation on surfaces for immunoassays, it is often difficult to evaluate the binding of antibodies derived from phage display libraries depleted and selected by panning on cell lines and living tumor cells. Performing cell membrane based ELISA methods does not reveal any up front kinetic binding information and depends on the performance of secondary antibodies and substrates. To overcome these limitations, we developed a new method to visualize direct antibody-cell membrane interactions by surface plasmon resonance using the Biacore 3000 and on-line signal subtraction on antigen-negative cell membrane vesicles. Conditions for the coating of cell membrane preparations to a carboxymethyl dextran hydrogel surface of a commercially available chip and the proof of concept for this application by the analysis of different formats of anti-CD30 and anti-carcinoembryonic antigen (CEA) antibodies interacting with coated membrane vesicles of CD30-positive/CEA-negative and CD30-negative and CEA-positive cell lines are described.
3 citations
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TL;DR: Single molecule array digital immunoassay platform has sensitivity about 1000-fold higher than traditional ligand binding assays, and report specifications to quantitate neurofilament light chain, a bona-fide biomarker for neurodegeneration.
Abstract: Aim: Globally, neurodegeneration accounts for significant morbidity and mortality among the elderly. Millions of people are afflicted with neurodegenerative diseases, with the most notable cases attributed to Alzheimer's, Huntington's, amyotrophic lateral sclerosis and Parkinson's diseases. Sensitive assays that can detect proteopathic anomalies indicative of early neurodegeneration have remained elusive. Therefore, there is an urgent need for sensitive diagnostic and prognostic biomarker assays that can guide the therapeutic regimen in the clinic. Materials & methods: Single molecule array digital immunoassay platform has sensitivity about 1000-fold higher than traditional ligand binding assays. Consequently, we are now beginning to implement ultrasensitive techniques in bioanalysis. Conclusion: In the current study, we evaluated single molecule array technology and report specifications to quantitate neurofilament light chain, a bona-fide biomarker for neurodegeneration. Preliminary neurofilament light screening results from 100 human geriatric cerebrospinal fluid samples displayed huge biological variation and warrants further investigation.
2 citations
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07 Jun 20192 citations
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Ben-Gurion University of the Negev1, University of Nairobi2, University of Washington3, Utrecht University4, University of Maryland Medical Center5, University of Otago6, University of Alberta7, Medical Research Council8, Vanderbilt University9, Vilnius University10, Ministry of Health and Family Welfare11, University of California, San Diego12, World Health Organization13, Pharmaceutical Product Development14, University of Colorado Denver15, Imperial College London16, University of Nigeria, Nsukka17, Novartis18
2 citations
Authors
Showing all 403 results
Name | H-index | Papers | Citations |
---|---|---|---|
Liangbing Hu | 128 | 480 | 61244 |
Evan A. Stein | 80 | 340 | 36392 |
Steven J. Schwartz | 75 | 313 | 17613 |
Debra A. Schaumberg | 62 | 154 | 15505 |
Lynda A. Szczech | 58 | 175 | 13972 |
Kim L. R. Brouwer | 57 | 247 | 12521 |
Robert S. Wallis | 57 | 147 | 10420 |
Marina A. Dobrovolskaia | 43 | 122 | 10915 |
Al Artaman | 38 | 41 | 61792 |
Bindu Kalesan | 38 | 123 | 8523 |
Stefan Barth | 34 | 238 | 4509 |
Yu.N. Makarov | 32 | 214 | 3578 |
Earl Hubbell | 28 | 76 | 12553 |
Alex Aravanis | 27 | 74 | 5230 |
Izabela Konczak | 24 | 47 | 1770 |