Institution
Stratagene
About: Stratagene is a based out in . It is known for research contribution in the topics: Nucleic acid & Gene. The organization has 161 authors who have published 232 publications receiving 17223 citations.
Topics: Nucleic acid, Gene, DNA polymerase, Nucleic acid methods, DNA
Papers published on a yearly basis
Papers
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14 May 2007TL;DR: In this article, a thermally-conductive reaction tube was proposed for containing reaction constituents during thermal cycling of the polymerase chain reaction (PCR) in the field of chemistry and molecular biology.
Abstract: The present invention relates generally to the fields of chemistry and molecular biology. More particularly, the present invention relates to thermally-conductive compositions and reaction tubes for chemical- and biochemical-based analytical processing. Compositions and reaction tubes in accordance with the invention comprise at least one plastic and at least one compound having a higher thermal conductivity than the at least one plastic to result in compositions and tubes having increased thermal conductivity when compared to the at least one plastic alone. Such compositions and tubes are capable of facilitating rapid heat transfer in numerous heat transfer applications. The thermally-conductive compositions and reaction tubes are especially suitable for containing reaction constituents during thermal cycling of the polymerase chain reaction (PCR).
19 citations
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10 Sep 2004TL;DR: In this article, a chemically reactive surface able to covalently react with substances containing a hydroxyl group and/or amine group is presented, comprising a solid surface having an activated dendrimer polyamine covalent bonded to said surface through a silane containing reagent.
Abstract: The present invention provides a chemically reactive surface able to covalently react with substances containing a hydroxyl group and/or amine group, comprising a solid surface having an activated dendrimer polyamine covalently bonded to said surface through a silane containing reagent, wherein the dendrimer polyamine can covalently bind the substance comprising a hydroxyl group and/or amino group. The present invention further provides a method for producing chemically reactive surfaces for binding moieties comprising a hydroxyl group and/or amine group, as well as kits comprising the chemically reactive surface of the invention.
19 citations
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23 Nov 1993TL;DR: In this article, the authors describe methods and materials for the detection of mycoplasma infections using polynucleotide primer pairs that are capable of hybridizing to mycplasma tRNA genes so as to provide for the generation of a distinctive set of amplification products.
Abstract: The invention described herein consists of methods and materials for the detection of mycoplasma infections. Mycoplasma infections may be detected in cell cultures and in animals. The subject methods use polynucleotide primer pairs that are capable of hybridizing to mycoplasma tRNA genes so as to provide for the generation of a distinctive set of amplification products when the primers are used in a cyclic amplification synthesis reaction, such as PCR (polymerase chain reaction). In addition to detecting mycoplasma infections, the subject methods may be used to identify the particular species of mycoplasma causing the infection. The subject invention also provides for primers and kits for performing the subject methods.
18 citations
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02 Sep 1993TL;DR: In this paper, the first strand of DNA complementary to a selected RNA or DNA template was synthesized by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA/DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog.
Abstract: The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The method comprises synthesizing a first strand of DNA complementary to a selected RNA or DNA template by contacting with the template a linker/primer comprising a selected restriction site, a suitable RNA or DNA dependent DNA polymerase, and substrates comprising a deoxyribonucleotide triphosphate analog. The linker/primer and deoxyribonucleotide triphosphate analog are selected such that incorporation of the nucleotide analog in the first strand substantially protects the double stranded DNA from cleavage, under conditions sufficient to cleave or substantially cleave the linker/primer, at the selected restriction site.
18 citations
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22 Feb 2001TL;DR: In this article, the authors provided methods of covalently joining nucleic acid molecules and methods of molecular cloning, and provided either sequential or simultaneous ligation of flanking or vector nucleic acids to nucleic insert molecules by topoisomerase and DNA ligase.
Abstract: The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
17 citations
Authors
Showing all 161 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ronald W. Davis | 155 | 644 | 151276 |
Michael Wigler | 113 | 298 | 63159 |
Michael McClelland | 79 | 372 | 27627 |
Jay M. Short | 41 | 66 | 13812 |
Joseph A. Sorge | 29 | 85 | 3981 |
Michael P. Weiner | 28 | 51 | 14127 |
Quinn Lu | 21 | 43 | 1955 |
John Welsh | 20 | 27 | 8075 |
Joseph A. Sorge | 15 | 36 | 2593 |
Bahram Arezi | 12 | 18 | 561 |
Eric J. Mathur | 12 | 59 | 1023 |
Natalia Novoradovskaya | 10 | 24 | 2527 |
Holly H. Hogrefe | 10 | 15 | 525 |
Jeffrey C. Braman | 10 | 16 | 1084 |
Patricia L. Kretz | 8 | 11 | 1155 |