Institution
Stratagene
About: Stratagene is a based out in . It is known for research contribution in the topics: Nucleic acid & Gene. The organization has 161 authors who have published 232 publications receiving 17223 citations.
Topics: Nucleic acid, Gene, DNA polymerase, Nucleic acid methods, DNA
Papers published on a yearly basis
Papers
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11 Feb 2004
TL;DR: In this article, a vector for the expression of an array of tRNA genes that are rare in the host cells is introduced. But the modified host cells are capable of efficiently supporting expression of selected recombinant genes which otherwise would be limited by the presence of rare codons.
Abstract: High level expression of heterologous proteins in host cells is frequently limited by the presence of rarely utilized codons, due to depletion of the internal tRNA pool and stalling of translation. This invention provides expression host cells generated by introduction of a vector for the expression of an array of tRNA genes that are rare in the host cells. The modified host cells are capable of efficiently supporting expression of selected recombinant genes which otherwise would be limited by the presence of rare codons.
4 citations
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05 Dec 2003TL;DR: In this article, the authors proposed a method for detecting the amount of a target polynucleotide using the subject composition, which involves the use of both target-hybridizing and non-target hybridizing probes, where the probes generate a detectable signal (e.g., detectable by FRET).
Abstract: The present invention relates to compositions comprising probes for the detection of a target polynucleotide, and methods for detecting the amount of a target polynucleotide using the subject composition. In particular, the subject method involves the use of both target-hybridizing probes and non-target-hybridizing probes, where the probes generate a detectable signal (e.g., detectable by FRET) for the target polynucleotide detection.
4 citations
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TL;DR: An epitope-tagging vector, pCMV-Tag1, is constructed, which allows for N-terminal, C-Terminal and internal tagging of the gene product of interest with the FLAG and/or c-myc epitopes, and enables researchers to rapidly and efficiently characterize gene products in vivo.
Abstract: We have constructed an epitope-tagging vector, pCMV-Tag 1, for gene expression in mammalian cells. This vector, which allows for N-terminal, C-terminal and internal tagging of the gene product of interest with the FLAG® and/or c-myc epitopes, enables researchers to rapidly and efficiently characterize gene products in vivo.
4 citations
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28 Jun 1993TL;DR: A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material, and a selected portion of the sample may then be collected as mentioned in this paper.
Abstract: An apparatus for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.
4 citations
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11 Aug 2000TL;DR: In this paper, a cell line comprising a stably integrated recombinant nucleic acid construct comprising a sequence encoding a fusion protein, a sequence-specific DNA binding domain, and a conditionally active transactivation domain of CHOP is presented.
Abstract: The invention encompasses compositions and methods which utilize a cell line comprising a stably integrated recombinant nucleic acid construct comprising: a reporter gene operably linked to a recognition sequence for a sequence-specific DNA-binding protein; and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a fusion protein, the fusion protein comprising a sequence-specific DNA binding domain, wherein the DNA binding domain specifically binds the recognition sequence, and a conditionally active transactivation domain of CHOP, wherein binding of the fusion protein to the recognition sequence results in transactivation of the reporter gene when the transactivation domain fused to the DNA binding domain is activated.
4 citations
Authors
Showing all 161 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ronald W. Davis | 155 | 644 | 151276 |
Michael Wigler | 113 | 298 | 63159 |
Michael McClelland | 79 | 372 | 27627 |
Jay M. Short | 41 | 66 | 13812 |
Joseph A. Sorge | 29 | 85 | 3981 |
Michael P. Weiner | 28 | 51 | 14127 |
Quinn Lu | 21 | 43 | 1955 |
John Welsh | 20 | 27 | 8075 |
Joseph A. Sorge | 15 | 36 | 2593 |
Bahram Arezi | 12 | 18 | 561 |
Eric J. Mathur | 12 | 59 | 1023 |
Natalia Novoradovskaya | 10 | 24 | 2527 |
Holly H. Hogrefe | 10 | 15 | 525 |
Jeffrey C. Braman | 10 | 16 | 1084 |
Patricia L. Kretz | 8 | 11 | 1155 |