Institution
Stratagene
About: Stratagene is a based out in . It is known for research contribution in the topics: Nucleic acid & Gene. The organization has 161 authors who have published 232 publications receiving 17223 citations.
Topics: Nucleic acid, Gene, DNA polymerase, Nucleic acid methods, DNA
Papers published on a yearly basis
Papers
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15 May 1995TL;DR: In this article, the cloning of DNA using genetically engineered host cells has been studied, and the host organisms of this invention are suitable for cloning DNA capable of forming non-standard and tertiary structures such as found in eukaryotic DNA.
Abstract: Methods and materials for the cloning of DNA, in particular, for the cloning of "unclonable" DNA using genetically engineered host cells. Host cell organisms have been discovered that stabilize and inhibit rearrangement of DNA molecules capable of forming non-standard secondary and tertiary structures. Organisms are engineered to contain at least one mutation which inactivates homologous recombination and at least one mutation in a DNA repair pathway. Examples of such DNA pathways include UV repair pathway, the SOS repair pathway, the mismatch repair pathway, the adaptive response pathway, the heat shock response pathway, the osmotic shock response pathway, the repair pathway of alkylation damage, the repair pathway of uracil incorporation into DNA and pathways involved in maintaining DNA superhelicity. The host organisms of this invention are suitable for cloning DNA capable of forming non-standard and tertiary structures such as found in eukaryotic DNA.
12 citations
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26 Feb 2001TL;DR: In this paper, the authors introduced recombinant polynucleotides encoding the Green Fluorescent Protein (GFP) from R. reniformis, as well as polynuclideans encoding variants and fusion polypeptides of GFP.
Abstract: The invention relates to recombinant polynucleotides encoding the Green Fluorescent Protein (GFP) from R. reniformis, as well as polynucleotides encoding variants and fusion polypeptides of R. reniformis GFP. The invention further relates to vectors encoding R. Reniformis GFP and variants and fusions thereof, as well as to cells comprising and/or expressing such vectors. The invention also relates to recombinant R. reniformis GFP polypeptides and fusion polypeptides and variants thereof, as well as to methods of making and using such polypeptides both in vivo and in vitro.
12 citations
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30 Dec 1992TL;DR: A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein, is described in this paper.
Abstract: A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
12 citations
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12 Nov 2004TL;DR: In this paper, the authors provide polynucleotides and vectors for detecting and/or isolating protein complexes or identifying a binding partner for a protein of interest, and for the chimeric proteins encoded by these polynuclidean nucleotides.
Abstract: The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
12 citations
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04 Jan 2006TL;DR: In this article, a thermally inactivated blocking polymerase protein is used to prevent access to a thermostable nucleic acid polymerase, thereby preventing the denature of the blocking polymerases.
Abstract: The invention relates to compositions, methods, and kits for hot start polynucleotide synthesis, including extension of primed polynucleotide templates and polymerase chain reaction (PCR). Hot start is provided by a thermally inactivated blocking polymerase protein that binds primed polynucleotide templates and prevents their access to a thermostable nucleic acid polymerase. High temperatures employed in the synthesis reaction cause the blocking polymerase to denature, thereby permitting the action of a thermostable processive polymerase. Compositions of the invention include a specific blocking polymerase protein which is a mutant of the Klenow fragment of E. coli DNA polymerase. The mutant is essentially devoid of polymerase activity, processivity, and 3' to 5' exonuclease activity. Use of the thermally inactivated blocking polymerase together with a thermostable polymerase reduces non-specific priming and accumulation of unwanted amplification products, increasing the specificity and sensitivity of the synthesis reaction.
12 citations
Authors
Showing all 161 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ronald W. Davis | 155 | 644 | 151276 |
Michael Wigler | 113 | 298 | 63159 |
Michael McClelland | 79 | 372 | 27627 |
Jay M. Short | 41 | 66 | 13812 |
Joseph A. Sorge | 29 | 85 | 3981 |
Michael P. Weiner | 28 | 51 | 14127 |
Quinn Lu | 21 | 43 | 1955 |
John Welsh | 20 | 27 | 8075 |
Joseph A. Sorge | 15 | 36 | 2593 |
Bahram Arezi | 12 | 18 | 561 |
Eric J. Mathur | 12 | 59 | 1023 |
Natalia Novoradovskaya | 10 | 24 | 2527 |
Holly H. Hogrefe | 10 | 15 | 525 |
Jeffrey C. Braman | 10 | 16 | 1084 |
Patricia L. Kretz | 8 | 11 | 1155 |