Institution
Stratagene
About: Stratagene is a based out in . It is known for research contribution in the topics: Nucleic acid & Gene. The organization has 161 authors who have published 232 publications receiving 17223 citations.
Topics: Nucleic acid, Gene, DNA polymerase, Nucleic acid methods, DNA
Papers published on a yearly basis
Papers
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TL;DR: Two mutations, 1226 and 1448, and a new mutation (XOVR) representing cross-over between the glucocerebrosidase gene and its closely linked pseudogene, were found in 47 unrelated patients with type I Gaucher's disease.
280 citations
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266 citations
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20 Oct 1994TL;DR: In this paper, the authors proposed a method in which a temperature gradient is generated across a gradient block, and an apparatus comprising a block (2, 17, 18, 19) across which a gradient can be generated.
Abstract: A method in which a temperature is generated across a gradient block, and an apparatus comprising a block (2, 17, 18, 19) across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells (3) on the gradient block (2, 17, 18, 19) can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment, the gradient block (2, 17, 18, 19) is integrated into a thermal cycler used for nucleic acid amplification reactions.
233 citations
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TL;DR: These unique expression patterns of Wnt family genes in different cell types of endometrium and the differential regulation of the inhibitors during the proliferative and secretory phase of the menstrual cycle strongly suggest functions for a Wnt signaling dialog between epithelial and stromal components in humanendometrium.
Abstract: Members of the Wnt family of signaling molecules are important in cell specification and epithelial-mesenchymal interactions, and targeted gene deletion of Wnt-7a in mice results in complete absence of uterine glands and infertility. To assess potential roles of the Wnt family in human endometrium, an endocrine-responsive tissue, we investigated in the proliferative and secretory phases of the menstrual cycle, endometrial expression of several Wnt ligands (Wnt-2, Wnt-3, Wnt-4, Wnt-5a, Wnt-7a, and Wnt-8b), receptors [Frizzled (Fz)-6 and low-density lipoprotein receptor-related protein (LRP)-6], inhibitors [FrpHE and Dickkopf (Dkk)-1], and downstream effectors (Dishevelled-1, glycogen synthase kinase-3beta, and beta-catenin) by RT-PCR, real-time PCR and in situ hybridization. No significant menstrual cycle dependence of the Wnt ligands (except Wnt-3), receptors, or downstream effectors, was observed. Wnt-3 increased 4.7-fold in proliferative compared with secretory endometrium (P < 0.05). However, both inhibitors showed dramatic changes during the cycle, with 22.2-fold down-regulation (P < 0.05) of FrpHE and 234.3-fold up-regulation (P < 0.001) of Dkk-1 in the secretory, compared with the proliferative phase. In situ hybridization revealed cell-specific expression of different Wnt family genes in human endometrium. Wnt-7a was exclusively expressed in the luminal epithelium, and Fz-6 and beta-catenin were expressed in both epithelium and stroma, without any apparent change during the cycle. Both FrpHE and Dkk-1 expression were restricted to the stroma, during the proliferative and secretory phase, respectively. These unique expression patterns of Wnt family genes in different cell types of endometrium and the differential regulation of the inhibitors during the proliferative and secretory phase of the menstrual cycle strongly suggest functions for a Wnt signaling dialog between epithelial and stromal components in human endometrium. Also, they underscore the likely importance of this family during endometrial development, differentiation and implantation.
197 citations
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TL;DR: A short term, in vivo mutagenesis assay has been developed utilizing a lacl target gene contained within a lambda ZAP shuttle vector which has been incorporated into transgenic mice, to allow for extrapolation of the extensive pool of in vitro data to whole animals, and provide insight into the tissue specific effects of mutagenic compounds.
Abstract: A short term, in vivo mutagenesis assay has been developed utilizing a lacl target gene contained within a lambda ZAP shuttle vector which has been incorporated into transgenic mice. Following chemical exposure, the target gene was recovered from mouse genomic DNA by mixing the DNA with in vitro lambda phage packaging extract. Mutations within the lacl target were identified by infecting host E. coli with the packaged phage and plating on indicator plates containing Xgal. Phage plaques with mutations in the lacl appeared blue, while intact phage were colorless. The ratio of blue plaques to colorless plaques is a measure of the mutagenicity of the compound. This system was used to obtain significant induction (up to 74-fold) over background levels for a variety of compounds, including N-ethyl-N-nitrosourea, benzo(a)pyrene (BaP), cyclophosphamide, and methylnitrosourea. Sequence analysis of selected mutant clones derived from this system was accomplished through the use of partial filamentous phage origins which allow rapid transfer of the target gene from phage to plasmid. Sequence analysis of spontaneous mutants derived from the mice primarily found of base substitutions, differing markedly from the previous data for spontaneous mutations in lacl derived from E. coli, where the preponderance of mutations were found at a single site, a repeated tetramer sequence. Upon sequence analysis of BaP derived base substitutions, only transversions were obtained, consistent with the known mechanism of BaP mutagenesis. Use of the well-characterized lacl gene in transgenic mice should allow for extrapolation of the extensive pool of in vitro data to whole animals, as well as provide insight into the tissue specific effects of mutagenic compounds.
194 citations
Authors
Showing all 161 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ronald W. Davis | 155 | 644 | 151276 |
Michael Wigler | 113 | 298 | 63159 |
Michael McClelland | 79 | 372 | 27627 |
Jay M. Short | 41 | 66 | 13812 |
Joseph A. Sorge | 29 | 85 | 3981 |
Michael P. Weiner | 28 | 51 | 14127 |
Quinn Lu | 21 | 43 | 1955 |
John Welsh | 20 | 27 | 8075 |
Joseph A. Sorge | 15 | 36 | 2593 |
Bahram Arezi | 12 | 18 | 561 |
Eric J. Mathur | 12 | 59 | 1023 |
Natalia Novoradovskaya | 10 | 24 | 2527 |
Holly H. Hogrefe | 10 | 15 | 525 |
Jeffrey C. Braman | 10 | 16 | 1084 |
Patricia L. Kretz | 8 | 11 | 1155 |