University of Hyderabad

About: University of Hyderabad is a education organization based out in Hyderabad, India. It is known for research contribution in the topics: Catalysis & Crystal structure. The organization has 6446 authors who have published 13005 publications receiving 237641 citations. The organization is also known as: Hyderabad Central University & HCU.

Betulinic acid binding to human serum albumin: A study of protein conformation and binding affinity

Abstract: Betulinic acid (BA) has anti cancer and anti-HIV activity and has been proved to be therapeutically effective against cancerous and HIV-infected cells. Human serum albumin (HSA) is the predominant protein in the blood. Most drugs that bind to HSA will be transported to other parts of the body. Using micro TOF-Q mass spectrometry, we have shown, for the first time that BA isolated from a plant (Tephrosia calophylla) binds to HSA. The binding constant of BA to HSA was calculated from fluorescence data and found to be KBA = 1.685 ± 0.01 × 106 M−1, indicating a strong binding affinity. The secondary structure of the HSA–BA complex was determined by circular dichroism. The results indicate that the HSA in this complex is partially unfolded. Further, binding of BA at nanomolar concentrations of BA to free HSA was detected using micro TOF-Q mass spectrometry. The study revealed a mass increase from 65199 Da (free HSA) to 65643 Da (HSA + drug), where the additional mass of 444 Da was due to bound BA. Based on the results of this study, it is suggested that micro TOF-Q mass spectrometry is useful technique for drug binding studies.

Photosynthesis in Heavy Metal Stressed Plants

Abstract: Use of phytotoxicity of metallic compounds dates back to 1896, when French farmers applied Bordeaux mixture (copper sulfate, lime and water) to control fungal pests (Martin and Woodcock 1983). Currently, global heavy metal (HM) pollution is a serious environmental concern.

Pseudopolymorph: retain this widely accepted term

Abstract: The author advocates use of the term pseudopolymorph. (1) The word has been around for 40 years. (2) Its meaning is clear enough to researchers in chemistry, pharmacology, and crystallography. (3) ...