Showing papers in "Anaerobe in 2004"

TL;DR: The diagnosis of C. perfringens infections in animals is complex and it is appropriate to rely on a combination of diagnostic techniques rather than one singe test.

TL;DR: This study, the first to provide information on the identities of methanogen colonising pre-ruminants, suggests that the predominant methanogens found in the mature rumen establish very soon after birth and well before a functioning rumen develops.

TL;DR: Phylogenetic analysis of archaea in the rumen ecosystem was analysed by PCR of 16S rDNA from the bovine rumen using archaea-specific primers to find the predominant family of whole rumen archaea was found to belong to the Methanomicrobiaceae.

TL;DR: Based on phenotypic and phylogenetic considerations, it is proposed that the unusual coccoid-shaped organism be classified as a new genus and species, Subdoligranulum variabile.

TL;DR: In this study, the in vitro effect of different substrates supplied to human faecal micro-flora, on the short-chain fatty acids (SCFA), including butyrate, which affects several cellular functions (proliferation, membrane synthesis, sodium absorption) and on the primary BA conversion rate is investigated.

TL;DR: It is proposed that the unknown bacterium isolated from dog feces be classified as a new genus and species, Allobaculum stercoricanis, based on both phenotypic and phylogenetic criteria.

TL;DR: A protease-nicked and biotinylated derivative of perfringolysin O (BCtheta) is prepared that binds selectively to cholesterol in cholesterol-rich microdomains of cell membranes without causing membrane lesions and BIAcore analysis showed that D4 binds specifically to cholesterol with the same binding affinity as the full-size toxin.

TL;DR: In the group of patients treated with antibiotics, resistant P. acnes strains were significantly more often isolated from antibiotic-treated patients with moderate to severe acne than from non-antibiotic- treated patients (odds ratio, 3.8; P=0.01).

TL;DR: Genome sequencing revealed that clostridia do not contain components of the so-called phosphorelay, with the exception of the essential transcription factor Spo0A, and an important regulatory function of a dual palindrome region upstream of the spo0A gene of C. acetobutylicum was revealed.

TL;DR: CPE is a unique, multifunctional toxin with cytotoxic, TJ-damaging, and (probably) proinflammatory activities and may also contribute to the GI symptoms of patients whose intestines contain high CPE levels.

TL;DR: With lower toxin levels, or in partially immune sheep, a focal, bilaterally symmetrical encephalomalacia sometimes occurs in selectively vulnerable brain regions after a more protracted clinical course, but the pathogenesis is uncertain.

TL;DR: The wide diversity of isolated anaerobic genera and species and the susceptibility patterns of the isolates emphasize the role of the an aerobic microbiology in cases of pleural empyema.

TL;DR: Diagnostic findings suggest that type E may play a significant role in enteritis of neonatal calves, and consideration should be given to type E-associated enteritis when planning for the health care of calves.

TL;DR: Data support that the genes of the botulinum neurotoxin complex are temporally expressed during late-log and early stationary phase and that toxin complex formation depends on the strain and growth medium.

TL;DR: On the basis of differences in genotypic and phenotypic characteristics, strain E1(T) (DSM 15206(T), CIP 107666(T)) is proposed as the type strain of a new species, C. tunisiense sp.

TL;DR: Studies on the effect of ammonia on maximum methanogenic activity of anaerobic biofilms enriched by methylaminotrophic methane producing Archaea (mMPA) showed that at 848.8 mg NH(3)-N/L, biofilm methane production required 36 h adaptation period, prior to entering into maximum production phase.

TL;DR: The botulinum genome contains predicted two-component system genes, some of which are possibly involved in regulation of toxinogenesis, and sigma factors related to TxeR and UviA, which regulate C. difficile toxin and C. perfringens bacteriocin production.

TL;DR: This minireview focuses on an analysis of the genome sequence of C. tetani E88, a vaccine production strain, which is a toxigenic non-sporulating variant of strain Massachusetts, a Gram-positive spore-forming bacterium predominantly found in the soil.

TL;DR: The study illustrated the advantage of using mixtures of C(12) and C(14) in ruminant nutrition to suppress methane emission since mixtures will reduce the amounts of the less palatable C( 12) required in feed.

TL;DR: It is proposed that the unknown bacterium be classified as C. bartlettii sp.

TL;DR: The hypothesis that the decrease in short-chain fatty acid concentration following antibiotic therapy predisposes patients to diarrheas caused by C. perfringens is supported.

TL;DR: It is proposed that the unknown bacterium from pig manure be classified in a new genus and species, as Atopostipes suicloacale gen. nov, based on both phenotypic and phylogenetic considerations.

TL;DR: It seems that prebiotic di- and oligosaccharides may have both a species and intra-species/strain selective effect and B. choerinum appeared to be well adapted to the gut of pre-weaned piglets.

TL;DR: Clostridium perfringens phospholipase C (Cp-PLC), the major virulence factor in the pathogenesis of gas gangrene, is a Zn(2+) metalloenzyme with lecithinase and sphingomyelinase activities.

TL;DR: The minimum inhibitory concentration (MIC) of eight antimicrobial agents was determined by the agar dilution method for 80 isolates of Clostridium difficile from neonatal pigs with enteritis as mentioned in this paper.

TL;DR: Because of its ability to resist the effects of tetracycline, this organism may be useful as a probiotic for the improved management of yeast related conditions such as thrush and irritable bowel syndrome.

TL;DR: Based on both phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from feces be classified in a new genus Anaerofustis, as Anaer ofustis stercorihominis sp.

TL;DR: The B. pilosicoli genome size was about 2.5 Mb, nearly 750 kb smaller than the B. hyodysenteriae genome as mentioned in this paper, which may be due to an unusual phage-like gene transfer agent, VSH-1.

TL;DR: Growth of the periodontal pathogen Bacteroides forsythus in broth cultures showed inhibition in the presence of 10 mM glucose added to the medium, an effect that must be considered in the design of optimal media for the culture of this fastidious species.

TL;DR: Milk-yeast extract (MYE) was shown to be the more suitable protective medium to maintain viability of the strains during the storage, and the vaginal Lactobacillus strains are most stable in MYE at -70 degrees C with only a small decrease of the viability observed under these conditions.