Showing papers in "Enzyme and Microbial Technology in 2019"

TL;DR: Silica has been extracted from rice husks via a simple hydrothermal process and functionalized with triethoxy(octyl)silane -OCTES (Octyl-SiO2) and (3-aminopropyl)triethoxysilane - 3-APTES (Amino-Si O2), with the aim of using it as support to immobilize lipase from Thermomyces lanuginosus via adsorption.

TL;DR: This is the first report exploring the molecular properties of PAD from thermotolerant fungi, to imitate the human temperature, and shows that PADs from E. dentata and A. nidulans had a relatively similar conformational and kinetic properties.

TL;DR: Results showed potential for liposome-mediated delivery of endolysin for exogenous application against Gram-negative bacteria.

TL;DR: The detection result in human serum suggested Cat-HNFs can be a promisng candidate for the detection of H2O2 in biomedical field.

TL;DR: The results indicate that strategies for increasing Chisb expression contribute to the study and application of chitinase and the production of ch itooligosaccharides.

TL;DR: This is first report exploring thermostable ADI from thermophilic A. nidulans with robust structural stability, catalytic efficiency and proteolytic resistance.

TL;DR: In this article, magnetic combi-CLEA has been synthesized by adding amino-functionalized magnetic nanoparticles into pectinase ultra-clear (containing pectinsases, xylanases and cellulases).

TL;DR: The data collected suggest that the role of the WSC domain of PoAlcOX is not recognition of substrates but attaching the enzyme to plant and/or fungal cell wall, and the carbohydrate-binding property of the domain is demonstrated.

TL;DR: An ATP regeneration system using polyphosphate kinase (ppk) is introduced into systems containing cadaverine decarboxylase (CadA) and PdxY for a sufficient supply of PLP, which resulted in enhanced cADAverine production and the use of surfactants was tested to improve transport efficiency.

TL;DR: The relative abundance of genera Pseudomonas, Rivibacter and Nitrobacter at the stress of Cu(II) and TC were higher than those at TC stress, suggested they had an ability to resist the stress.

TL;DR: Using Fe-MIL-88(NH2) as a support to immobilize Luciferase established simple immobilization process of luciferase with and without linker and it improved the remaining activity significantly.

TL;DR: The nanoparticles of commercially available pyruvate oxidase (POx) from Aerococcus species were prepared by desolvation method, which were then characterized and covalently immobilized onto gold electrode (AuE) to construct an improved model of amperometric pyruVate biosensor, which displayed an excellent correlation coefficient.

TL;DR: π-TAIC from Ilumatobacter coccineus was identified for the production of 3-ATfBA and was successfully used for the synthesis of various β-amino acids from their corresponding β-keto ester substrates.

TL;DR: For the first time in this study, one microorganism was immobilized onto the electrospun nanofiber mat by covalent modification.

TL;DR: It proved that the immobilization of horseradish peroxidase (HRP) could improve the performance of HRP, which had a good prospect in biological application.

TL;DR: Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5 to enhance the activity for oxidation of phenolic and lignin substrates, and mutants showed enhanced product release and enhanced thermostability.

TL;DR: This study sought to enhance the stability and reusability of this whole-cell system for realizing the efficient production of glutaric acid under harsh reaction conditions and selected a PVA-PEG gel (LentiKats®) for cell entrapment, and several factors of the reaction were optimized.

TL;DR: The resulting Kraton/MWCNTs/Frt/GOx bioande exhibited good catalytic activity towards glucose oxidation along with excellent stability and can be employed to develop other polysaccharide or oligosaccharide fuel cells in which glucose oxidation is involved.

TL;DR: The halophilic green alga Dunaliella bardawil FACHB-847 is rich in lutein and α-carotene, which has great potential for carotenoid production in open ponds, and genes encoding lycopene β- and ε-cyclases (DbLcyB and DbLcyE) were functionally identified by genetic complementation in E. coli.

TL;DR: Three amino acid sites in Bman26 were found to have close relationships with the enzyme's thermal stability, which will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.

TL;DR: Recombinant GHs45 from diverging subfamilies stand out for differences in substrate specificity appearing as new tools for preparation of enzyme cocktails used in cellulose hydrolysis and useful to enhance initial glucan hydrolytic rates during saccharification of sugarcane pith by a mixture of cellulolytic enzymes.

TL;DR: It was shown that Rex8As are key enzymes in the efficient saccharification of hetero-xylan into xylose, a major component of lignocellulosic substrates, and synergistic enhancement with respect to reducing sugar release was observed.

TL;DR: Results showed that glutamate feeding facilitated glucose consumption and further led to the increment of astaxanthin content with little influence of cell growth in X. dendrorhous as a response to the glutamate feeding.

TL;DR: Results suggest that ASase can be used to synthesize EMIQ in a simple and specific process.

TL;DR: The recombinant chitinase from C. violaceum ATCC 12472 inhibited the conidia germination of the phytopathogenic fungi Fusarium oxysporum and F. guttiforme, hence preventing mycelial growth and showing the potential of CvChi47 as a molecular tool to control plant diseases caused by these Fus aquarium species.

TL;DR: The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking and was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.

TL;DR: The kinetics data conclusively showed that CRL/CS-NC was catalytically more efficient than free CRL (Vmax 0.48 mM min-1, Keff 0.07 min-1’mM-1) and Butanol was preferred over butyric acid as reflected by the higher apparent Michaelis-Menten constant of CRL/.

TL;DR: Overall, the simultaneous use of CRISPR-δ-integration and multiple promoter shuffling can be useful and easily applied for recombinant protein production.

TL;DR: This is the first report about the covalent co-immobilization of TA and PLP with high activity and stability, and this work also contributed to the economic flexibility of chiral amine pharmaceutical intermediates biosynthesis.

TL;DR: SMM resulted in stronger interaction between IL anions and glucose than WMM, which was attributed to greater solubility of sugar in ILs prepared by SMM, which suggested methanol aided sugar solvation in hydrophobic media.