Showing papers in "European Journal of Cell Biology in 1995"

TL;DR: It is demonstrated, after subcellular fractionation procedures, that MDC-labeled organelles contained the lysosomal enzymes acid phosphatase and the mature form of cathepsin D, and Membrane markers of rough endoplasmic reticulum (TRAM and sec61 beta), and for smooth endoplasmsic reticle (cytochrome P450) were not detected in the same fractions.

TL;DR: Strains of Drosophila melanogaster with differing immune capabilities against the wasp parasitoid Leptopilina boulardi were examined for superoxide anion (O2-.) formation during parasitization, indicating that neither the quinoid precursors of melanin nor O2-.

TL;DR: The aim of this article is to summarize the sequential events that occur during wound healing notably cell migration, proliferation and phenotypic differentiation and to describe the cellular interactions involving growth factors at the molecular level.

TL;DR: It is demonstrated that in addition to its location in the early endocytic and recycling pathway, gp330 is also present in microvilli and the protein and degradation products thereof is present in lysosomes, consistent with its role as a protein scavenger receptor.

TL;DR: Using three unrelated agents to induce apoptosis in thymocytes from 10-day-old BALB/c mice, evidence is suggested that a decrease in [GSH]i, or an increase in [ GSSG]i or perhaps a change in the ratio of [G SHi to [GSSGi]i constitutes a trigger for apoptosis.

TL;DR: Morphometric analysis of observations showed that the nature of the phagocytic particle affects phagosome processing: as long as particles remain undigested, maturation of phagosomes is prevented and they remain fusogenic towards early endosomes; concurrent to particle digestion, phagosom processing proceeds towards transfer of phagcytic contents to phagolysosomes which display kinetic and compositional characteristics of lysosomes.

TL;DR: It is shown that certain cells such as the epidermal keratinocyte line HaCaT and the vulvar carcinoma-derived line A-431 continually synthesize more than one Dsc subtype, and the utilization of reagents for the specific Dsg and D sc subtypes in tumor diagnosis is proposed.

TL;DR: The data suggest that delivery of internalized molecules to lysosomal proteolysis takes place by fusion of mature endosomes with preexisting lysOSomes and that actin microfilaments somehow facilitate this step.

TL;DR: TGF beta 1, PDGF, bFGF and IGF-1 are all capable of increasing collagen deposition by cardiac fibroblasts by either stimulating fibroblast replication or collagen synthesis or both, which is consistent with their playing a role in the rapid changes in cardiac collagen deposition seen during development and disease.

TL;DR: Modulation of the epithelial phenotype of uterine cells, i.e. loss of apical-basal polarity, might prepare the apical cell pole for cell-cell interaction with trophoblast if accompanied by a loss of epithelium-specific adhesion molecules.

TL;DR: The results clarify previous confusion in the literature regarding the processing of filensin which arose because of the similar relative electrophoretic mobilities by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the different fragment sets.

TL;DR: The results suggest that Leishmania amazonensis PKC activity may modulate its interaction with macrophages via secreted AcP.

TL;DR: In resistant cells, inhibition of P-glycoprotein activity was able to induce drug accumulation in the nuclei of resistant cells and to achieve cytotoxic effects comparable to those observed in sensitive cells, suggesting the possible involvement of nuclear P- glycoprotein in the regulation of subcellular doxorubicin distribution in multidrug-resistant cells.

TL;DR: Treatment with 20 microM brefeldin A for 60 min caused the disassembly of the Golgi apparatus in tobacco BY-2 cells, and the effect of BFA was reversible.

TL;DR: It is suggested that the cytoplasmically disposed nucleoporin RanBP2 provides docking sites for import substrate-receptor complexes and, further, that the affinity of these sites to the transport substrate is modulated in a Ran-dependent fashion.

TL;DR: The differential cellular expression of moesin and its pronounced occurrence on microspikes of growing cells support the possibility that moesIn is a protein involved in plasma membrane-cytoskeleton interactions in specialized tissues.

TL;DR: Gutmann et al. as discussed by the authors examined the effect of microtubule disruption by colchicine on 1) the subcellular organization of the apical endocytic apparatus, 2) apical Endocytosis, and 3) sub-cellular distribution of gp330 and Aquaporin-1 water channels in renal proximal tubule cells.

TL;DR: It is concluded that in class III IF proteins stepwise deletions in the tail, while not considerably altering IF assembly in vitro, can change the topogenesis of IF proteins and structures in the living cell.

TL;DR: It was found that the proliferative growth of various nontransformed cell lines does not only depend on cell adhesion to a suitable substratum and cell flattening but also on intact microfilaments, and the significance of microfilm organization for growth regulation is discussed.

TL;DR: Results suggest that bFGF associated to HSPG can enter the cell via several pathways and follows mainly a degradative route.

TL;DR: Results documented the convergence of anterograde transport and retrograde transport at the 15 degrees C compartment and implied the existence of a segregation or a sorting process that would result in the segregation of proteins with different targeting signals in the structure.

TL;DR: In this paper, the expression of lamin A and C (A-type lamins) in a lung carcinoma cell line using type-specific monoclonal antibodies was studied using immunofluorescence and immunoblotting studies.

TL;DR: Vicia sativa ssp.

TL;DR: This study has developed a method to enrich AL from enucleated oocytes, and has quantified a PC marker protein, nucleoporin p62, in both cytoplasmic AL and the NE, showing that the number of PCs and p62 molecules present in AL far exceeds that of the NE.

TL;DR: Immunofluorescence and immunoelectron microscopy of parasitized mouse peritoneal macrophages and infected mouse tissue suggest that the proteophosphoglycan is secreted in large amounts by amastigotes via their flagellar pockets into the parasitophorous vacuoles of host cells.

TL;DR: Using anti-myosin antibodies, standard immunocytochemical techniques in conjunction with confocal scanning laser microscopy and colloidal gold immunoelectron microscopy, changes in the distribution patterns of myosin during the early stages of pollen hydration, germination, tube growth, and myosIn associated with isolated vegetative nucleus and the generative cell in Nicotiana tabacum L.

TL;DR: The fact that the synthesis patterns of immature neurons from newborns does not differ from that of mature neurons indicates that the changes in the synthesis pattern of core histones occur at the arrest of cell proliferation and are unrelated to the state of differentiation of the cells.

TL;DR: It is demonstrated that LPS increases tyrosine phosphorylation of 19 and 22 kDa polypeptides in C. capitata hemocytes, supporting the hypothesis that tyrosinesine protein phosphorylated is a signal reaction in hemocytes after LPS exposure.

TL;DR: Interactions of chondrocytes with their extracellular matrix are an absolute requirement for the expression of angiogenic activities by hypertrophic chondROcytes at late developmental stages.

TL;DR: The development of a simple and reliable immunocytochemical labeling protocol should allow for the further analysis of leukocyte microtubules as related to the normal functional response of these cells and form the basis for correlating alterations in microtubule dynamics with the effects of taxol on leukocytes function.