Showing papers in "Journal of Food Science in 2019"
TL;DR: The results indicated that EPW3 possessed great therapeutic potential as adjuvant therapy for type 2 diabetes, as indicated by activation of the IRS1/PI3K/AKT and inhibition of the JNK1/2 insulin pathway in liver.
Abstract: This study aimed to investigate the antidiabetic activity of water?ethanol extract of green macroalgae Enteromorpha prolifera (EPW) and its flavonoid-rich fraction less than 3 kDa (EPW3) in type 2 diabetic mice induced by streptozotocin and a high-sucrose/high-fat diet. The major active compounds were identified using ultra-performance liquid chromatography coupled with quadrupole-time-of-flight-tandem mass spectrometry. Quantitative gene expression analysis of the insulin signaling pathway was performed. The effects of EPW3 on gut microflora in diabetic mice were analyzed by high-throughput 16S rRNA gene sequencing. The results showed EPW3 treatment decreased the fasting blood glucose, improved oral glucose tolerance, and protected against liver and kidney injury with reduced inflammation in diabetic mice. The active principle of EPW3 revealed hypoglycemic effect as indicated by activation of the IRS1/PI3K/AKT and inhibition of the JNK1/2 insulin pathway in liver. Furthermore, the treatment significantly enriched the abundance of Lachnospiraceae and Alisties, which were positive correlation of metabolic phenotypes. These findings indicated that EPW3 possessed great therapeutic potential as adjuvant therapy for type 2 diabetes.
89 citations
TL;DR: The present review collects the most recent evidences of the health risks of dietary protein oxidation and proposes reasonable hypotheses and future perspectives on the field.
Abstract: The exposure to reactive oxygen species (ROS) is an inevitable consequence of living in an aerobic world. The species contribute to the occurrence of oxidative stress in humans in which an uncontrolled production of ROS exceeds the endogenous antioxidant defences leading to the oxidative damage to essential cellular components, such as lipids, proteins, and DNA. The influence of diet on the modulation of the systemic redox status is recognized and, while some dietary components are found to be protective (that is, fruits and vegetables), others are recognized as pro-oxidants (that is, processed meat and other animal-source protein foods). Oxidized proteins and amino acids are potential promoters of luminal and postprandial oxidative stress; preliminary studies have actually reported noxious effects of these species in cultured cells and in experimental animals. However, the underlying pathological mechanisms remain poorly understood. The application of advanced methodological approaches based on mass spectrometric technologies and OMICS disciplines has enabled the elucidation of the molecular basis of the pathological effects of dietary oxidized proteins and amino acids. The present review collects the most recent evidences of the health risks of dietary protein oxidation and proposes reasonable hypotheses and future perspectives on the field.
79 citations
TL;DR: Film incorporating metallic nanoparticles showed antibacterial properties against Escherichia coli and Staphylococcus aureus growth and can be used as active packaging films and could increase the shelf-life of the food.
Abstract: In this study, silver (Ag), zinc oxide (ZnO), and copper oxide (CuO) metallic nanoparticles were used in preparation of carboxymethyl cellulose (CMC) nanobiocomposite films. Scanning electron microscopy (SEM), X-ray diffraction (EDXA), water vapor permeability (WVP), ultraviolet and visible light (UV-Vis) spectroscopy, and mechanical and microbial tests were used to determine the characteristics of the obtained active films. SEM results showed that the CMC nanobiocomposite films had roughness deflection levels and the EDXA test confirmed the presence of Ag, ZnO, and Cuo nanoparticles in the biopolymer tissue. UV-Vis spectroscopy confirmed that with addition of metallic nanoparticles to the pure CMC film, absorption rate increased and WVP decreased. In the mechanical tests, addition of nanoparticles also increased the tensile strength of the films, and the nanobiocomposite films exhibited higher resistance compared to the pure CMC film. Films incorporating metallic nanoparticles showed antibacterial properties against Escherichia coli and Staphylococcus aureus growth. Thus, nanobiocomposite films can be used as active packaging films and could increase the shelf-life of the food.
69 citations
TL;DR: It is concluded, that the electrospraying technique made possible the obtention of quercitin-loaded zein nanoparticles increasing their bioavailability and can be used to decrease the prevalence of chronic degenerative diseases by increasing of the bioavailability ofQuercetin in the bloodstream.
Abstract: Quercetin is a hydrophobic flavonoid with high antioxidant activity. However, for biological applications, the bioavailability of quercetin is low due to physiological barriers. For this reason, an alternative is the protection of quercetin in matrices of biopolymers as zein. The objective of this work was to prepare and characterize quercetin-loaded zein nanoparticles by electrospraying and its study of in vitro bioavailability. The physicochemical parameters such as viscosity, density, and electrical conductivity of zein solutions showed a dependence of the ethanol concentration. In addition, rheological parameters demonstrated that solutions of zein in aqueous ethanol present Newtonian behavior, rebounding in the formation of nanoparticles by electrospraying, providing spherical, homogeneous, and compact morphologies, mainly at a concentration of 80% (v/v) of ethanol and of 5% (w/v) of zein. The size and shape of quercetin-loaded zein nanoparticles were studied by transmission electron microscopy (TEM), observing that it was entrapped, distributed throughout the nanoparticle of zein. Analysis by Fourier transform-infrared (FT-IR) of zein nanoparticles loaded with quercetin revealed interactions via hydrogen bonds. The efficacy of zein nanoparticles to entrap quercetin was particularly high for all quercetin concentration evaluated in this work (87.9 ± 1.5% to 93.0 ± 2.6%). The in vitro gastrointestinal release of trapped quercetin after 240 min was 79.1%, while that for free quercetin was 99.2%. The in vitro bioavailability was higher for trapped quercetin (5.9%) compared to free quercetin (1.9%), than of gastrointestinal digestion. It is concluded, that the electrospraying technique made possible the obtention of quercitin-loaded zein nanoparticles increasing their bioavailability. PRACTICAL APPLICATION: This type of nanosystems can be used in the food and pharmaceutical industry. Quercetin-loaded zein nanoparticles for its improvement compared to free quercetin can be used to decrease the prevalence of chronic degenerative diseases by increasing of the bioavailability of quercetin in the bloodstream. Other application can be as an antioxidant system in functional foods or oils to increase shelf life.
55 citations
TL;DR: Blackcurrants are a rich source of phytochemicals with potent antioxidant, antimicrobial, and anti-inflammatory properties and have the potential to improve overall human health particularly with diseases associated with inflammation and regulation of blood glucose.
Abstract: Blackcurrants (BC; Ribes nigrum) are relatively new to the U.S. market; however, they are well known and popular in Europe and Asia. The use of BC has been trending worldwide, particularly in the United States. We believe that demand for BC will grow as consumers become aware of the several potential health benefits these berries offer. The objectives of this review were to provide an up-to-date summary of information on BC based on articles published within the last decade; furthermore, to provide the food industry insights into possibilities for the utilization of BC. The chemistry, processing methods, and health benefits have been highlighted in addition to how the environment and variety impact the chemical constituents of BC. A search for journal publications on BC was conducted, which included keywords such as chemical characterization, health benefits, processing, technologies, anthocyanins (ANC), and proanthocyanidins. This review provides up-to-date information available on the subject. In conclusion, BC and their products have industrial uses from which extractions can be made to produce natural pigments to be used as food additives. BC contain flavonoids, specifically ANC, which provide the fruits with their purple color. BC are a rich source of phytochemicals with potent antioxidant, antimicrobial, and anti-inflammatory properties. Also, BC have the potential to improve overall human health particularly with diseases associated with inflammation and regulation of blood glucose.
51 citations
TL;DR: The results indicate that mitochondrial degeneration, depletion of metabolites that produce NADH, and increased oxidative stress can limit shelf-life of aged steaks and application of post-harvest strategies to minimize mitochondrial damage and oxidative changes may have the potential to increase shelf- life of aged beef.
Abstract: Interrelationship between mitochondria and myoglobin function influence beef color. NADH level in postmortem muscle is an important determinant of mitochondrial activity and metmyoglobin reduction. Increased aging time promotes discoloration of steaks; however, the mechanism of this effect is not clear. The objective was to characterize the role of wet-aging in beef longissimus lumborum muscle mitochondrial function and to characterize the global metabolome to determine the mechanism of that can regenerate NADH. Beef longissimus lumborum muscles were randomly assigned to 3, 7, 14, 21, and 28 days aging periods. Surface color, biochemical, mitochondrial, and metabolite profiles were determined at each aging period and at the end of 6-day display. During 6-day display, sections aged for 28 days had 30.4% decrease in redness than sections aged for 3 days. Aging time decreased (P <0.05) muscle oxygen consumption, mitochondrial protein content, and antioxidant capacity. Metabolites such as fumaric acid, creatinine, and fructose, that can take part in glycolytic/TCA cycle and regenerate NADH decreased (P <0.05) with aging and display time. In support, NADH levels also decreased (P <0.05) with aging time, but aging time had no effect (P = 0.44) on NADH-dependent reductase activity. The results suggest that decreased color stability in aged beef can be attributed to increased mitochondrial damage, depletion of metabolites that can regenerate NADH, and increased oxidative stress. PRACTICAL APPLICATION: Beef aging time results in increased discoloration of steaks under retail display. The current research determines the fundamental basis of lower color stability in aged beef. The results indicate that mitochondrial degeneration, depletion of metabolites that produce NADH, and increased oxidative stress can limit shelf-life of aged steaks. Hence, application of post-harvest strategies to minimize mitochondrial damage and oxidative changes may have the potential to increase shelf-life of aged beef.
51 citations
TL;DR: The results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides.
Abstract: Four polysaccharide fractions (P-1: 71.40%, P-2: 1.95%, P-3: 1.14%, P-4: 1.64%) were isolated from crude Polygonatum sibiricum polysaccharide (PSP), processed by water extraction, ethanol precipitation, and further separated with diethylaminoethyl cellulose-52 anion-exchange chromatography. Their molecular weights and monosaccharide compositions were characterized by high performance gel chromatography with evaporative light scattering detector and ultraviolet-visible detector. The antioxidant activity of four polysaccharides fractions were assessed by the electron transfer menchanism (DPPH, ferric reducing power, and ABST assays) and chelation of transition metals (Fe2+ and Cu2+ chelation ability). The highest content fraction P-1 exhibited the lowest antioxidant activity, and the ranking of antioxidant capacity was P-4 > P-3 > P-2 > PSP > P-1. After processed by microwave-assisted degradation, the molecular weight of P-1 was decreased from 2.99 × 105 to 2.33 × 103 Da, while the antioxidant activity of degraded P-1 was about eightfold higher than natural P-1. These results indicated that the proposed microwave-assisted degradation approach was an efficacious methodology to improve their bioactivity by lower the molecular weight of polysaccharides. PRACTICAL APPLICATION: This study provided an environmentally friendly, convenient and efficient microwave-assisted degradation technology to process the neutral polysaccharides from Polygonatum sibiricum. The results could be used for the development and utilization of various plant polysaccharides as a kind of food supplement in our daily life.
51 citations
TL;DR: The electrospraying process is a feasible technique for obtaining gallic acid-loaded zein nanoparticles and providing potential protection togallic acid from environmental factors.
Abstract: Currently, electrospraying is a novel process for obtaining the nanoparticles from biopolymers. Zein nanoparticles have been obtained by this method and used to protect both hydrophilic and hydrophobic antioxidant molecules from environmental factors. The objective of this work was to prepare and characterize gallic acid-loaded zein nanoparticles obtained by the electrospraying process to provide protection to gallic acid from environmental factors. Thus, it was related to the concentration of gallic acid in physicochemical and rheological properties of the electrosprayed solution, and also to equipment parameters, such as voltage, flow rate, and distance of the collector in morphology, and particle size. The physicochemical properties showed a relationship in the formation of a Taylor cone, in which at a low concentration of gallic acid (1% w/v), low viscosity (0.00464 ± 0.00001 Pa·s), and density (0.886 ± 0.00002 g/cm3 ), as well as high electrical conductivity (369 ± 4.3 µs/cm), forms a stable cone-jet mode. The rheological properties and the Power Law model of the gallic acid-zein electrosprayed solution demonstrated Newtonian behavior (n = 1). The morphology and size of the particle were dependent on the concentration of gallic acid. Electrosprayed parameters with high voltage (15 kV), low flow rate (0.1 mL/hr), and short distance (10 cm) exhibited a smaller diameter and spherical morphology. FT-IR showed interaction in the gallic acid-loaded zein nanoparticle by hydrogen bonds. Therefore, the electrospraying process is a feasible technique for obtaining gallic acid-loaded zein nanoparticles and providing potential protection to gallic acid from environmental factors.
50 citations
TL;DR: The current methods of LBA production, patents related, general applications and regulations, research statistics, future prospects, and an overview of the challenges faced by the food industry to incorporate the acid in their products are described.
Abstract: Lactobionic acid (LBA) is a bionic acid naturally found in the "Caspian Sea yogurt" and chemically constituted of a gluconic acid bonded to a galactose. The compound is known for its numerous proven attributes as an antioxidant, chelator, and moisturizer agent. There is a growing interest of the academic community and industry research sectors in the application of LBA as a food ingredient. Thus, this review describes the current methods of LBA production, patents related, general applications and regulations, research statistics, future prospects, and an overview of the challenges faced by the food industry to incorporate the acid in their products. Studies associated to food application and human intake are scarce in the literature. To date, they account for only a small amount of all available research papers and patents on the subject, which is due to LBA prohibitions despite the approval of its salt (calcium lactobionate) and the lack of regulation in most countries. Further studies on the safety of consumption should be carried out in coming years in order to elucidate its toxicological aspects and to extend the technological possibilities of the food processing industry.
50 citations
TL;DR: Application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen and be able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract.
Abstract: Chitosan from squid (Loligo formosana) pens were prepared and characterized. First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM). Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein. Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr). When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%. Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 105 to 3.2 × 105 Da, respectively. All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract. Chitosan produced by deacetylation at 130 °C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 105 Da). DDA of CH130-2 determined using 1 H-NMR was 89%, which was similar to that (87%) obtained from FTIR. XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation. CH130-2 stabilized the emulsion under the simulated gastrointestinal conditions. Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract. PRACTICAL APPLICATION: Chitin and chitosan are marketable products manufactured from crustacean shells. However, extraction of chitin is time consuming. Ultrasonication has been used for extraction of various biomolecules from different sources. It effectively lowers the processing time and enhances extraction yield. Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen. Chitin from squid pen could be further converted to chitosan with high DDA. Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract. Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.
46 citations
TL;DR: BRS benefited the cholesterol and glucose metabolism, as well as optimized gut microbiota composition in mice fed with a high-fat diet, and direction for further research is given.
Abstract: Buckwheat-resistant starch (BRS) has shown to be a nutrient capable of lowering cholesterol and reducing obesity. In this study, the regulatory effects of tartary buckwheat starch on blood lipid level and gut microbiota (Lactobacillus, Bifidobacterium, Enterococcus, and Escherichia coli) in mice fed with a high-fat diet was investigated. Male C57BL/6 mice were separately fed with a normal diet (CON), a high-fat diet (HFD), and high-fat diet supplemented with buckwheat-resistant starch (HFD+BRS) for 6 weeks. After the feedings, lipid profile, blood glucose, plasma levels of cytokines, short-chain fatty acid content in the colon and intestinal flora of fecal were measured. Furthermore, the antioxidant indices of the liver and duodenum tissues were measured to evaluate the antioxidant capacity of mice. Significantly reduced plasma levels of total cholesterol (TC), triglyceride (TG), glucose, and cytokines were observed in the HFD+BRS group, accompanied by an increased antioxidant capacity in the liver and duodenum. In addition, supplementation with BRS significantly inhibited the increase in plasma lipopolysaccharide, tumor necrosis factor-α, and interleukin-6 levels. Gut microbiota composition was regulated by the supplement of BRS, which promoted the growth of Lactobacillus, Bifidobacterium, and Enterococcus, as well as inhibited the growth of Escherichia coli. In contrast to the HFD group, the content of short-chain fatty acids in mice colon increased in the BRS group. In conclusion, BRS benefited the cholesterol and glucose metabolism, as well as optimized gut microbiota composition in mice fed with a high-fat diet. PRACTICAL APPLICATION: This study identified the beneficial effects of tartary buckwheat-resistant starch on the regulation of blood lipids and intestinal flora in mice fed a high-fat diet. The result of this study will provide a basis for the development of probiotic products supplemented with tartary buckwheat-resistant starch and direction for further research.
TL;DR: This review provides a comprehensive overview about the isolation, physicochemical features, application, and functional properties and biological activities of Brewer's spent grain protein hydrolysates (BSGPHs).
Abstract: There is a growing demand in reusing agro-industrial waste to enrich food proteins and develop new protein-related products. Brewer's spent grain (BSG), the byproducts generated from the processing of beer-brewing industry, is rich in abundant protein (26 to 30%) with good physicochemical properties and nutritional benefits. BSG is mainly composed of four proteins including hordein, gluten, globulin, and albumin. The methods for extracting protein from BSG mainly include alkali extraction, ultrasonic-assisted extraction, and organic solvent extraction, among others. However, little researches have described the functional properties of Brewer's spent grain protein (BSGP) and how it can be improved by enzymatic modification. Additionally, BSG protein hydrolysates (BSGPHs) have good bioactivities, mainly including antioxidant, antiinflammatory, and angiotensin-I-converting enzyme inhibitory activities, among others. Based on the above situation, this review provides a comprehensive overview about the isolation, physicochemical features, application of the BSGP. In addition, the functional properties and biological activities of BSGPHs are also emphasized. The purpose of this review is to provide an up-to-date summary of BSGP research, and to broaden the market potential of this emerging protein in food industry. Besides, this review provides a reference to study the use of BSGP or hydrolysates for a variety of purposes in-depth, including pharmaceutical, food, and industrial applications.
TL;DR: Results demonstrate that cooling rate, MG concentration, and HIU can be used satisfactorily to tailor physical properties of MG oleogels, while high-intensity ultrasound (HIU) is a relatively novel technique to tailor lipid properties.
Abstract: The aim of this study was to investigate the effects of monoglycerides (MG) concentration (3, 4.5, and 6 wt%), cooling rate (0.1 and 10 °C/min), and high-intensity ultrasound (HIU) application on physical properties of oleogels from MG and high oleic sunflower oil. Microstructure, melting profile, elasticity (G'), and solid fat content (SFC) were measured immediately after preparation of samples (t = 0) and after 24 hr of storage at 25 °C. Samples' textural properties (hardness, adhesiveness, and cohesiveness) and oil binding capacity (OBC) were evaluated after 24 hr at 25 °C. In general, samples became less elastic over time. Slow cooling rate resulted in lower G' after 24 hr compared to the ones obtained using 10 °C/min. Network OBC was improved by increasing MG concentration and cooling rate, and by applying HIU. After storage, oleogel melting enthalpy increased with MG concentration. In general, this behavior was not correlated with an increase in SFC. An improvement in the network structure was generally reached with the increase in cooling rate, according to texture and rheology results, for both sonicated and nonsonicated conditions. At the highest MG concentration, HIU application was more efficient at increasing OBC and hardness of the network at 0.1 °C/min. Microscopy images showed that the oleogels microstructure was changed as a consequence of HIU application and cooling rate, evidencing smaller crystals both in sonicated and faster cooled samples. Obtained results demonstrate that cooling rate, MG concentration, and HIU can be used satisfactorily to tailor physical properties of MG oleogels. PRACTICAL APPLICATION: Oleogels have been studied in the last years as semisolid fat replacers in food products. Cooling rate is an important processing parameter in the oleogel preparation because it affects their final physical properties, while high-intensity ultrasound (HIU) is a relatively novel technique to tailor lipid properties. This study is focused on the application of a slow/fast cooling rate in combination with/without HIU treatment at different monoglycerides and high oleic sunflower oil mixtures as a successful strategy to obtain oleogels with different physical properties and with potential applications in the food industry, such as fat substitutes in bakery.
TL;DR: The anticancer property was found to be the most promising biological activity of turnip with 2-phenylethyl isothiocyanate, phenylpropionitrile, brassicaphenanthrene A, 6-paradol, and trans-6-shogaol as the major active constituents.
Abstract: Despite the advancement of medical science, diseases are part-and-parcel of human life. Plants have provided humans with medicines since time immemorial, and are still one of the primary sources for drug discovery. Brassica rapa L., commonly known as turnip, is one of the world's oldest cultivated vegetables. Besides being an important vegetable and source of oil, turnip is also used as a traditional medicine for the treatment of headaches, chest complaints, rheumatisms, oedemas, gonorrhoea, syphilis, and rabies. Glucosinolates and isothiocyanates (mainly 2-phenylethyl, 4-pentenyl, and 3-butenyl derivatives) are the main constituents of turnip with diverse bioactivities, especially for the protective effect against cancers. Besides, flavonoids, phenolics, indoles and volatiles are also concomitant in this plant. Pharmacological investigation on turnip revealed the antitumor, antihypertensive, antidiabetic, antioxidant, antiinflammatory, hepatoprotective, and nephroprotective effects. The anticancer property was found to be the most promising biological activity of turnip with 2-phenylethyl isothiocyanate, phenylpropionitrile, brassicaphenanthrene A, 6-paradol, and trans-6-shogaol as the major active constituents. Flavonoids and phenolics with high free radical scavenging activity should be corresponding to the antioxidant effects. Arvelexin, an indole derivative in turnip, was reported with various effects involving antiinflamatory, antihypertensive and hypolipidemic potency. In spite of many studies concerning either the chemical constituents or the biological activities of turnip, only a few cases disclosed the active ingredients responsible for diverse bioactivities. This review summarizes the research progress on the chemistry and health-benefits of turnip over the past 20 years to provide a reference for the further investigation.
TL;DR: This research addressed some of the global need for clarification and harmonization of commonly used terminology and presented common definitions and publication details to gain insight that could help provide clarity.
Abstract: The food industry is advancing at a rapid pace and consumer sensitivity to food safety scares and food fraud scandals is further amplified by rapid communication such as by social media. Academia, regulators, and industry practitioners alike struggle with an evolving issue regarding new terms and definitions including food fraud, food authenticity, food integrity, food protection, economically motivated adulteration, food crime, food security, contaminant, adulterant, and others. This research addressed some of the global need for clarification and harmonization of commonly used terminology. The 150 survey responses were received from various food-related workgroups or committee members, communication with recognized experts, and announcements to the food industry in general. Overall food fraud was identified as a "food safety" issue (86%). The food quality and manufacturing respondents focused mainly on incoming goods and adulterant-substances (<50%) rather than the other illegal activities such as counterfeiting, theft, gray market/diversion, and smuggling. Of the terms included to represent "intentional deception for economic gain" the respondents generally agreed with food fraud as the preferred term. Overall, the preference was 50% "food fraud," 15% "economically motivated adulteration" EMA, 9% "food protection," 7% "food integrity," 5% "food authenticity," and 2% "food crime." It appears that "food protection" and "food integrity" are terms that cover broader concepts such as all types of intentional acts and even possibly food safety or food quality. "Food authenticity" was defined with the phrase "to ensure" so seemed to be identified as an "attribute" that helped define fraudulent acts. PRACTICAL APPLICATION: Food Fraud-illegal deception for economic gain using food-is a rapidly evolving research topic and is facing confusion due to the use of different terms and definitions. This research survey presented common definitions and publication details to gain insight that could help provide clarity. The insight from this report provides guidance for others who are harmonizing terminology and setting the overall strategic direction.
TL;DR: CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can be used in the food packaging industry, making them suitable for use as packaging materials.
Abstract: To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo). Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films. The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined. The results confirmed all of the films exhibited excellent UV protection with low transparency at 600 nm. Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength. SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM. Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability. The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%. The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively. These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising. PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable. The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials. CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.
TL;DR: Findings in bamboo leaf extracts antioxidants suggested that BFE intervention upregulated related antioxidant defense responses for preventing cells from oxidative damage.
Abstract: In this study, bamboo leaf flavonoids extracts (BFE) were employed to alleviate oxidative stress induced by oleic acid in HepG2 cells. Biochemical indexes, intracellular reactive oxygen species production, lipid droplets accumulation, antioxidant enzymes production, and mitochondrial membrane potential were determined to show the alleviation performance of BFE intervention (P < 0.05). Importantly, the results of qRT-PCR and western blot determination indicated that BFE intervention upregulated the expression of Nrf2/HO-1/NQO1 to initiate the antioxidant defense response for counteracting oxidative stress (P < 0.05). Moreover, mitochondrial membrane potential-mediated apoptosis and FOXO signaling pathway initiation caused by BFE intervention may together contribute to oxidative stress alleviation in HepG2 cells. In conclusion, these findings suggested that BFE intervention upregulated related antioxidant defense responses for preventing cells from oxidative damage. PRACTICAL APPLICATION: In this study, bamboo leaf flavonoids extracts intervention upregulated related antioxidant defense responses for preventing cells from oxidative damage. These findings in bamboo leaf extracts antioxidants are a promising and innovative subject with practical applications to enhance the development of bamboo leaf extracts functional products in the food industry.
TL;DR: It is concluded that lycopene protects against diabetic progression and prevents further complications of diabetic rats through ameliorating oxidative stress and inflammation, as well as improving the systemic antioxidative capacity.
Abstract: We aim to study the antioxidative and anti-inflammatory effects of lycopene on type 2 diabetes mellitus (T2DM) rats, anticipating a complementary strategy for the prevention of long-term complications of T2DM. In this study, rats with streptozotocin-induced diabetes were divided into four groups, receiving a 10-week lycopene intervention: DM, DM + low dose of lycopene (L), DM + medium dose of lycopene (M), and DM + high dose of lycopene (H) group with 0, 5, 10, and 15 mg/kg BW lycopene, respectively. At the end of intervention, fasted blood glucose (FBG) level, oxidative stress indicators, including glycosylated hemoglobin (GHb), glycosylated low-density lipoprotein, oxidized low-density lipoprotein (ox-LDL). and malondialdehyde (MDA), as well as antioxidants, that is, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and inflammatory factors like tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were determined. The results indicated that oxidative stress and inflammatory factors were elevated in DM rats. Lycopene intervention decreased the FBG level in DM rats compared with the untreated ones. It revealed a dose-dependent effect on decreasing serum oxidative stress biomarkers, including GHb, ox-LDL, and MDA. Inflammatory factors (TNF-α and CRP) in DM rats were also decreased by lycopene intervention. Total antioxidative capacity as well as the activities of antioxidants in DM rats including CAT, SOD, and GPx were increased after lycopene intervention. We conclude that lycopene protects against diabetic progression and prevents further complications of diabetic rats through ameliorating oxidative stress and inflammation, as well as improving the systemic antioxidative capacity. PRACTICAL APPLICATION: According to our study, lycopene intakes at experimental dosages appear to have beneficial effects on ameliorating oxidative stress and inflammation in type 2 diabetes mellitus (T2DM) rats, suggesting that lycopene might help improving T2DM progression when its daily intake is up to about 0.79 mg/kg BW in humans, which approximately equals to 5 mg/kg BW in rats. However, more clinical trials are needed to provide a more reliable and convincing conclusion in humans.; © 2019 Institute of Food Technologists®.
TL;DR: The e-tongue may be useful in qualitative discrimination among spicy paneer cheese samples along with sensory evaluation and could be used alongside sensory testing to evaluate spicy food samples without encountering the typical issues of fatigue common with evaluating spicy foods.
Abstract: Evaluating sensory properties of spicy foods can be challenging due to how quickly individuals experience fatigue. Analytical methods, such as HPLC quantification of capsaicin, are accurate. However, they may not always be interchangeable with perception of spiciness by consumers. The electronic tongue (e-tongue) offers a unique opportunity to simulate human perception of capsaicin pungency with an analytical method. This study evaluated consumer's and the e-tongue ability to discriminate among paneer cheese samples containing different levels of capsaicin (1.875, 3.75, 7.5, 15, and 30 ppm). Over 2 days, using a blocked design to minimize fatigue, consumers (n = 110) evaluated samples using a difference from control sensory test. Consumers distinguished the spicy paneer sample from the control (0 ppm) at 3.75, 7.5, 15, and 30 ppm (P < 0.05). Differences were found among sample 3.75, 7.5, and 15 ppm (P < 0.05). However, no significant differences were found between control and 1.875 ppm or between samples 15 and 30 ppm. Although these high and low concentrations were challenging for consumers, the e-tongue resulted in a high degree of discrimination of 93% among all samples. On the principal component analysis plot created by the e-tongue, PC1 explained 85.6% of the variability and was associated with spicy, sweet, salty, sour, and umami sensors. PC2 explained 7.96% and was associated with the bitter and metallic sensors. The 3.75 and 15 ppm samples were defined by PC1, while PC2 defined samples 1.875 and 30 ppm. These results conclude that the e-tongue may be useful in qualitative discrimination among spicy paneer along with sensory evaluation. PRACTICAL APPLICATION: As the market for spicy foods continues to grow, methods for rapid, accurate analysis of the sensory qualities of the food products is needed. As the electronic tongue discriminated effectively among spicy paneer cheese samples, the method could be used alongside sensory testing to evaluate spicy food samples without encountering the typical issues of fatigue common with evaluating spicy foods.
TL;DR: Thyme essential oil-loaded microcapsules fabricated using natural polysaccharide chitosan and sodium alginate as the shell material via the method of layer-by-layer (LBL) assembly could be a potential alternative as a natural antimicrobial agent in food and pharmaceutical industries.
Abstract: Thyme essential oil-loaded microcapsules (TMS) were fabricated using natural polysaccharide chitosan (CS) and sodium alginate (SA) as the shell material via the method of layer-by-layer (LBL) assembly. The accumulated release rates of thyme oil and microcapsules at 4 °C were 42.50% and 10.16%, respectively. After heating at 100 °C for 5 hr, the release rate of the 0, 2, 4, 6 layers assembled microcapsules were 100%, 48.84%, 28.38%, 19.3%, severally. Microcapsules also had good pH sensitivity in the range of 4 to 10. Antimicrobial function studies showed that the microcapsules are more effective than thyme oil for three tested microorganisms. When the temperature rose from 37 °C to 121 °C, the antibacterial zone of thyme oil gradually decreased from 18.5 ± 0.6 mm to 12.3 ± 0.6 mm, although inhibition rate of microcapsules increased from 87.97% to 99.75%. The antibacterial effect of thyme oil declined with the increase of pH, in terms of microcapsules, the efficiency was better under acidic or alkaline conditions. The thyme oil microcapsules can suppress the growth of Staphylococcus aureus in milk and prolong its shelf life. It was determined that this microcapsule could be a potential alternative as a natural antimicrobial agent in food and pharmaceutical industries. PRACTICAL APPLICATION: This work provided release performance and mechanism of layer-by-layer (LBL) thyme oil microcapsule under different conditions, and further studies showed its antibacterial ability to explore how herb essential oils can be potentially applied in food packaging and antibacterial areas.; © 2019 Institute of Food Technologists®.
TL;DR: The optimized extract, which was rich in anthocyanins, showed chemical and biological antioxidant activity (protection against erythrocyte hemolysis) and inhibited lipid peroxidation in vitro, and may be used as a natural colorant/antioxidant in foods.
Abstract: Red chicory leaves are appreciated sensorially and their constituents contain bioactive properties. The objectives of this study were as follows: to use an experimental design to extract anthocyanins from red chicory in aqueous solution at pH 2.5; to determine the stability of the extracts in relation to temperature and pH; and to evaluate the antioxidant activity and in vitro cytotoxic effect of the lyophilized and purified extracts. The best extraction conditions for the bioactive compounds from red chicory were a temperature of 64.2 °C for 25 min; the anthocyanin content was 73.53 ± 0.13 mg per 100 g fresh weight basis sample. The EC50 (Half maximal effective concentration) value for the antioxidant activity assay in relation to DPPH (2,2-diphenyl-1-picrylhydrazyl) with optimized extract was 0.363, which corresponds to a concentration of 39.171 µmol/L of anthocyanins. The activation energy for the degradation reaction of the anthocyanins from the red chicory extract was 84.88 kJ/mol. The optimized extract, which was rich in anthocyanins, showed chemical and biological antioxidant activity (protection against erythrocyte hemolysis) and inhibited lipid peroxidation in vitro. The Cichorium intybus L. extracts interfered on the levels of reactive oxygen species generation and the crude extract did not present procarcinogenic effect. PRACTICAL APPLICATION: Red chicory is basically consumed as a part of traditional dishes worldwide. Here, we developed a process to extract and purify the anthocyanins from Cichorium intybus leaves and test the extracts in terms of the chemical composition, thermal stability, antioxidant activity, and antiproliferative effects. The anthocyanin-rich extract presented antioxidant activity in chemical and biological assays and low cytotoxicity and cytoprotective effects in relation to HepG2, HCT8, and Caco-2 cell lines. Additionally, the red chicory extract protected human erythrocytes against hemolysis. This extract may be used as a natural colorant/antioxidant in foods.
TL;DR: The present study attempted to create a novel shortening by employing oleogels structured by ethylcellulose (EC), the most promising gelation agent to develop gel network capable of replacing solid fat without health concerns.
Abstract: A novel shortening was developed based on oleogels structured by ethylcellulose (EC) polymers. The texture and oil retention ability of EC oleogels were characterized against the level of viscosity of different grades of EC, as well as the rheological properties in relation to the polymer structure in the gel network. EC100, which has an average viscosity of 100 cP, was selected as the most suitable organogelator at 4% (w/w) in combination with base oil (30% degree of saturation by mixing palm stearin and soybean oil) to form the shortening. Triglyceryl monostearate (TMS) was found to be the most effective emulsifier as evidenced by its ability to strengthen air-incorporation ability of the shortening while creating evenly distributed fine crystals in the system. The EC100 shortening was able to create breads with excellent specific volume, indicating its ability to incorporate air bubbles during dough development and to serve as an antifirming agent to create bread with stable soft texture. PRACTICAL APPLICATION: In the present study, we attempted to create a novel shortening by employing oleogels structured by ethylcellulose (EC), the most promising gelation agent to develop gel network capable of replacing solid fat without health concerns. EC oleogels in shortening with detailed characterization of the shortening microstructure in relation to its functional properties was elucidated. The optimal formulation in relation to preservation of gel structure and consistency with enhanced moisture and air retention were also identified.
TL;DR: The gut microbiota of a healthy host is enough for the whole isoflavone metabolism under normal conditions, and a remarkable shift in gut microbiota after soymilk administration and antibiotics treatment is found.
Abstract: Lactobacillus rhamnosus strain ASCC 1520 with high soy isoflavone transformation ability was used to ferment soymilk and added to the diet of mice. The impact of L. rhamnosus fermentation on soy isoflavone metabolites and intestinal bacterial community, in conjunction with fecal enzyme activity and short-chain fatty acids (SCFA) excretion was evaluated. Antibiotics intervention resulted in a decrease in fecal enzyme activities and SCFA. Although long-term intake of soymilk or L. rhamnosus-fermented soymilk did not affect the fecal β-glucuronidase and β-galactosidase activities, it improved the β-glucosidase activity when antibiotics were concomitantly administered. Soymilk or fermented soymilk administration increased the isoflavone metabolites (O-DMA and equol) excreted in urine. Antibiotics decreased the daidzein excretion and its metabolites but showed little effect on glycitein and genistein excretion. Principal coordinates analysis (PCoA) of the 16s rRNA gene sequencing data found a remarkable shift in gut microbiota after soymilk administration and antibiotics treatment. Matastats test of the relative abundance of bacterial taxa revealed Odoribacter (Bacteroidales family), Lactobacillus (Lactobacillales order), and Alistipes (Rikenellaceae family) were enriched in soymilk while bacterial taxa from Bacteroides and Lactobacillus were enriched in L. rhamnosus-fermented soymilk. Furthermore, there was less decrease in bacterial taxa with fermented soymilk group even when antibiotics were concomitantly administered. Overall, this study revealed that the gut microbiota of a healthy host is enough for the whole isoflavone metabolism under normal conditions. Feeding mice with L. rhamnosus-fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used. PRACTICAL APPLICATION: Feeding mice with L. rhamnosus-fermented soymilk improved fecal enzyme activity and kept the balance of the gut mirobiota when antibiotics were used.
TL;DR: Correlation analysis amongorganic Se content, peptide content, and antioxidant activity revealed that organic Se plays a greater role in free radical scavenging than peptides, and that the organic Se content of the Se-enriched peptides was positively correlated with their antioxidant ability.
Abstract: Cardamine violifolia is a selenium (Se)-enriched plant found in China. In this study, the Se-enriched peptides of C. violifolia (CP) were isolated using a 1 kDa ultrafiltration membrane after enzymatic hydrolysis by alkaline and neutral proteases. The peptides were separated by DEAE-Sepharose FF anion-exchange chromatography and purified using preparative high-performance liquid chromatography (prep-HPLC). The component with the highest antioxidant activity, CPR13, was identified by comparing the 1,1-diphenyl-2-picrylhydrazyl (DPPH•), hydroxyl (•OH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+ •), and superoxide ( O 2 - · ) radical scavenging ability of each fraction. At a concentration of 0.1 mg/mL, the DPPH•, •OH, O 2 - · , and ABTS+ • scavenging activities of CPR13 were 89.2%, 26.3%, 40.6%, and 42.9%, respectively. Amino acid sequences were obtained by liquid chromatography combined with mass spectrometry as follows: GRVGSSSC, GRAGGSYM, GHPNFKLNCSGG, GTKSCKA, ASSNARDMI, TAGGCYIPI, and KNCALQ. The seleno-amino acids were identified as selenomethionine, methylselenocysteine, and selenocysteine. Correlation analysis among organic Se content, peptide content, and antioxidant activity revealed that organic Se plays a greater role in free radical scavenging than peptides, and that the organic Se content of the Se-enriched peptides was positively correlated with their antioxidant ability (P < 0.05). It indicated that CP has a great potential as natural functional materials for dietary supplement.
TL;DR: The results indicated that Guanzhong goat and Holstein cow milk were different in varieties of whey proteins and MFGM proteins and their functions and pathways, providing useful information for infant formula and milk protein products in the Chinese dairy industry.
Abstract: Guanzhong goat and Holstein cow milks are the major milk supply for the Chinese dairy industry. Whey proteins and milk fat globule membrane (MFGM) proteins of both milk were characterized and compared using proteomic techniques. A total of 283, 159, 593, and 349 proteins were identified, respectively, in whey and MFGM for the two species using Liquid Chromatography combined with Tandem Mass Spectrometry (LC-MS/MS). Functional categories analyses showed that both goat and cow MFGM proteins had three most abundant proteins of phosphoproteins, membrane-related and acetylation-related proteins. Gene ontology (GO) annotation revealed that whey proteins in goat and cow milk exhibited different biological processes and molecular functions while both enriched in extracellular exosome for cellular components. Both goat and cow MFGM proteins showed main biological process of oxidation-reduction, cellular component of extracellular exosome, and molecular function of poly(A) RNA binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed that large number of both goat and cow whey proteins were involved in disease, metabolism, and immune pathways with different number and types. The most general pathways for goat and cow MFGM proteins were metabolism pathways and disease pathways, respectively. The results indicated that Guanzhong goat and Holstein cow milk were different in varieties of whey proteins and MFGM proteins and their functions and pathways. PRACTICAL APPLICATION: Guanzhong goat and Holstein cow milks are the major milk sources for the Chinese consumers. However, information about proteomics of whey and MFGM proteins of Guanzhong goat and Holstein cow milk is limited. Our study characterized and compared both whey and MFGM proteins using proteomic techniques. The results provide useful information for infant formula and milk protein products in the Chinese dairy industry.
TL;DR: The Cahuacintle corn husk can be considered as a competitive source of anthocyanins with the available commercial sources and it is demonstrated that their anthcyanins profile is comparable to other Peruvian purple corns, which are currently used as natural colorants.
Abstract: Pigmented maize has been extensively studied due to its high anthocyanin content. This study has been focused mainly on kernel, although the whole plant of purple corn is a potential source of anthocyanins. First, general parameters of extraction (solvent system, solvent-to-solid ratio, number of extractions, and acid type) were established depending on the total anthocyanins content. Then, three extraction methods to access anthocyanins were compared: maceration extraction (ME), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE). Since the residual material still possessed an intense color, a further treatment was performed by application of enzymatic-assisted extraction (EAE). Three enzymatic cocktails (Xylanases, Celluclast, and Depol), pH, and temperature were evaluated to establish optimal reaction conditions. Subsequent analysis and identification of the anthocyanins obtained by four different extraction techniques were performed using HPLC and HPLC-mass spectrometry, respectively. The most efficient method was UAE using 20 min of ultrasound (100 W) preceded by sample treatment in the following conditions: ethanol/water/lactic acid mixture (80:19:1), two extractions, 1:10 solvent-to-solid ratio. As a result, anthocyanins from corn cob and corn husk were extracted at concentrations of 24.32 and 25.80 mg/gDW, respectively. No difference in the anthocyanins profile for samples extracted by three different methods was observed. However, an enhanced presence of cyanidin-3-(6''malonyl)glucoside was detected in the sample corresponding to the EAE method. Therefore, the Cahuacintle corn husk can be considered as a competitive source of anthocyanins with the available commercial sources. PRACTICAL APPLICATION: The by-products obtained from Cacahuacintle purple corn can be potentially used as natural colorants thanks to their anthocyanins content. In this work, we established the most efficient extraction method of anthocyanins from corn husk and corn cob, and demonstrated that their anthocyanins profile is comparable to other Peruvian purple corns, which are currently used as natural colorants. Therefore, the extraction procedure described in this study might be scaled-up in an industrial process to get access to anthocyanins from undervalued wastes.
TL;DR: It is suggested that fermented blueberries are rich in phenolic acids, which are a promising source of natural antioxidants and anticancer drugs and can be used as additives in food, pharmaceuticals, and cosmetic preparations.
Abstract: This study determined the effects of blueberry fermentation by Lactobacillus plantarum on antioxidant and anticancer activities The fermented blueberries extracted with 80% ethanol (FBE) showed increased superoxide dismutase-like activity, increased scavenging of DPPH and alkyl radicals, and increased antiproliferative activity against human cervical carcinoma HeLa cells by inducing apoptosis Seven representative phenolic compounds (malvidin 3-O-glucopyranoside, gallic acid, protocatechuic acid, catechol, chlorogenic acid, syringic acid, and epigallocatechin) in FBE were measured by high-performance liquid chromatography at different fermentation times The content of each phenolic compound in the FBE was dependent on the fermentation period Protocatechuic acid and catechol levels increased significantly with fermentation time Of these three major compounds (protocatechuic acid, catechol, and chlorogenic acid), catechol showed the most significant anticancer activity when HeLa cells were treated with each of these three compounds alone or mixed in various ratios Pearson's product-moment correlation analysis revealed that the increases in antioxidant and anticancer activities following blueberry fermentation were positively correlated with the phenolic acids present in FBE PRACTICAL APPLICATION: Blueberries fermented with a tannase-producing lactic acid bacteria (LAB), Lactobacillus plantarum showed higher antioxidant activities and antiproliferative activities against human cervical carcinoma HeLa cells than did raw blueberries L plantarum fermentation biotransformed blueberry polyphenols into active phenol metabolites with strong antioxidant and antiproliferative activities Our results suggest that fermented blueberries are rich in phenolic acids, which are a promising source of natural antioxidants and anticancer drugs and can be used as additives in food, pharmaceuticals, and cosmetic preparations
TL;DR: It is suggested that blending of chia oil is an adequate alternative to obtain ω-3-enriched oils with higher oxidative stability indices, and the blends developed in this study take advantage of the properties of each parent oil to yield products with improved oxidative stability, essential fatty acid presence, and sensory characteristics.
Abstract: Chia seed (Salvia hispanica L.) oil is mainly composed of ω-3 fatty acids (61% to 70%). Despite being nutritionally favorable, higher amounts of polyunsaturated fatty acids result in poorer oxidative stability. Thus, the aim of this work was to produce edible vegetable oil blends rich in ω-3 fatty acids and with greater oxidative stability than pure chia oil. Blending of chia with other specialty oils (walnut, almond, virgin, and roasted sesame oils) was assessed in the following respective proportions: 20:80, 30:70, and 40:60 (v/v). An accelerated storage test was conducted (40 ± 1 °C, 12 days). Primary and secondary oxidation products, free fatty acid content, antioxidant compounds, fatty acid composition, and induction time were determined. The blends presented higher oxidative stability indices than chia oil. Sensory analysis showed that, given a pure oil, judges did not identify statistically significant differences among the blends. The results suggest that blending of chia oil is an adequate alternative to obtain ω-3-enriched oils with higher oxidative stability indices. PRACTICAL APPLICATION: Vegetable oil blending is a widely used practice in the edible oil industry to produce blended oils with enhanced stability and nutritional and sensory properties at affordable prices. The blends developed in this study from chia, sesame, walnut, and almond oils take advantage of the properties of each parent oil to yield products with improved oxidative stability, essential fatty acid presence, and sensory characteristics. To achieve a daily intake of 2.22 g/day of ω-3 fatty acids as recommended by the Intl. Society for the Study of Fatty Acids and Lipids (ISSFAL), it is necessary to consume approximately one spoonful of the formulated mixtures.; © 2019 Institute of Food Technologists®.
TL;DR: DBD-HVCAP was demonstrated as an innovative technology for extending the shelf-life of Asian sea bass slices, which could be implemented in seafood industries for assuring safety and extending shelf- life of products.
Abstract: Impact of dielectric barrier discharge high-voltage cold atmospheric plasma (DBD-HVCAP) generated with the mixture of oxygen and argon (10:90) for various treatment times (2.5 to 10 min) on the qualities of Asian sea bass slices during 4 °C storage was investigated. Microbial load of slices treated with DBD-HVCAP were lower than the control. The efficacy of bacteria reduction by DBD-HVCAP was dependent on the treatment times (P < 0.05). Total viable bacteria count (TVBC) was more than 6.0 Log CFU/g at day 6 for the control kept in air. Slices treated with DBD-HVCAP for all treatment times used had TVBC lower than the limit at day 12. Total volatile nitrogen base content (TVNB) as well as trimethylamine (TMA) content in slices treated with DBD-HVCAP were lower than that of the control throughout the storage. TVNB as well as TMA contents were lower in HVCAP treated slices in a treatment time-dependent manner. Nevertheless, lipid oxidation in samples treated with DBD-HVCAP was higher than that of the control. Polyunsaturated fatty acids were decreased in slices treated with DBD-HVCAP for more than 5 min after 12 days of storage. Therefore DBD-HVCAP treatment for 5 min was demonstrated to be potential means for increasing the shelf-life of Asian sea bass slices with minimal negative effect on chemical and sensory properties, in which they could be stored longer than 12 days at 4 °C. PRACTICAL APPLICATION: Microbial inactivation capacity of dielectric barrier discharge high-voltage cold atmospheric plasma (DBD-HVCAP) has been documented with limited information on its application in extending the shelf-life of foods. DBD-HVCAP was demonstrated as an innovative technology for extending the shelf-life of Asian sea bass slices, which could be implemented in seafood industries for assuring safety and extending shelf-life of products. The shelf-life of the slices treated with DBD-HVCAP was extended to 12 days of storage at 4 °C as compared to the 6 days of the untreated counterpart.
TL;DR: Food safety managers in food service establishments may consider reevaluating their current food safety training program to incorporate behavioral-basedFood safety training in addition to knowledge-based training.
Abstract: Proper food safety training is essential to decrease incidences and overall rates of foodborne illnesses and outbreaks. Though many commercial restaurants should provide proper food safety training to food handler employees, this training is not always offered or effective. Here, we summarize the results of a primary literature study concerning the effectiveness of food safety training in commercial settings. The literature chosen for review contained only studies with experimental food safety training, with before and after training data. Through evaluation of these studies, the best practice for ensuring effective training and follow-through were the use of food safety training programs, which incorporated both knowledge and behavior-based training. PRACTICAL APPLICATION: Food safety managers in food service establishments may consider reevaluating their current food safety training program to incorporate behavioral-based food safety training in addition to knowledge-based training.; © 2019 Institute of Food Technologists®.