Showing papers in "Journal of Immunological Methods in 1993"

TL;DR: In this article, the carboxylic ionophore monensin was used to interrupt intracellular transport processes leading to an accumulation of the cytokine in the Golgi complex.

TL;DR: It would appear to be possible to use ATP bioluminescence in the detection of cytokine activity in a number of different bioassays.

TL;DR: The MTT assay for cell viability and cell proliferation has been modified to improve its reproducibility and accuracy and was used for evaluating the effects of different IL-2 concentrations on cell proliferation.

TL;DR: Its advantages over XTT/PMS, another tetrazolium which yields a water-soluble formazan product, include the absorbance range of color produced, the rapidity of color development, and the storage stability of the MTS/P MS reagent solution.

TL;DR: The utility of this technique lies in the ability to produce a relatively inexpensive customized reagent that is simple to use and that allows for sensitive determinations of gene expression in a rapid and convenient manner.

TL;DR: The results demonstrate that it is possible to use the MTT assay interchangeably with radioactive base techniques to measure cell proliferation and cytotoxicity and makes this method a serious contender for replacing the conventional radioactive techniques.

TL;DR: The water dilution method (WD) was compared with three other methods, namely, polyethylene glycol (PEG), dextran sulphate (DS) and xanthan gum (Xan) in terms of yield, purity, ease of use, potential scaling up and immunoactivity of IgY and was found superior.

TL;DR: The MTT assay for cell viability and cell proliferation has been modified to improve its reproducibility and accuracy and was used for evaluating the effects of different IL-2 concentrations on cell proliferation.

TL;DR: An automated micro-fluorometric assay based on the oxidation of nonfluorescent DCFH-DA to highly fluorescent 2',7'-dichlorofluorescein (DCF) both intracellularly and extracellularly to monitor oxidative burst in phagocytes has been developed.

TL;DR: A microfluorometric method for phagocytosis study has been developed using fluorescein conjugated Escherichia coli K-12 particles based on the uptake of fluorescent particles and quenching of extracellular fluorescence at the end of the assay.

TL;DR: The DHR flow cytometric assay is the most sensitive technique available for investigating changes that may occur in the oxidative activities and distribution patterns of active neutrophil subpopulations in response to a variety of physiological and pathological conditions.

TL;DR: The MODE-K cell line is a suitable model for the analysis of intestinal epithelial cell function in mucosal immunity and the ability to process and present native protein antigens to specific CD4+ T-cell hybridomas, via functional class II molecules is suggested.

TL;DR: A rapid quantitative fluorometric assay was developed for analysis of leukocyte adherence to endothelial cells and found the fluorescence assay to be more rapid as the use and special handling of radioactive material is eliminated.

TL;DR: These analyses using the dual approaches of immunoenzymetric and fluorescent immunohistochemical techniques provide important evidence that cytokine induction kinetics can differ following primary or secondary stimulation.

TL;DR: The results obtained with CRC1 show that antibodies useful for immunoassays can sometimes be obtained without the purified target molecule being available for immunization or screening, and the value of anti-peptide monoclonal antibodies in the study of inhibin is demonstrated.

TL;DR: Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity.

TL;DR: This new two-color flow cytometric technique has proven to be uncomplicated and reproducible when used in the clinical setting and direct correlations of normal individuals tested in parallel with the fluorescent dyes and the 51Cr-release assay have shown direct correlations.

TL;DR: In this paper, the results of phase I of an international effort to develop and standardize assays for the detection of anti-neutrophil cytoplasmic antibodies (ANCA) were described.

TL;DR: The XTT and MTT colorimetric bactericidal assays produced comparable results when used to measure bovine neutrophil bactericidal activity against S. aureus, E. coli, L. monocytogenes, and B. abortus.

TL;DR: It is demonstrated that it is possible to engineer antibodies and antibody fragments (Fab, Fv) with protein L-binding framework regions, which can then be utilized in aprotein L-based purification protocol.

TL;DR: Improved TGF-beta 1 SELISAs with comparable sensitivities to the above assay are described based on available sources of commercial monoclonal and polyclonal antibodies.

TL;DR: The potential of the ELISA procedure for evaluating complement activation in clinical samples was demonstrated and an ELISA for the quantification of C4b/c in biological fluids was developed.

TL;DR: It is concluded that double-step PCR employing nested primers is superior to other mycoplasma detection methods in many respects: simplicity and speed, high specificity and extreme sensitivity, objectivity and accuracy.

TL;DR: A modified polymerase chain reaction (PCR) assay for analysis of cytokine gene expression from reverse-transcribed RNA obtained from small numbers of cells is described in detail and can be used successfully for studying differential expression of IL-10 in different microenvironments during infection of mice with Schistosoma mansoni.

TL;DR: The Fab and Fab' fragments appeared to be similar on the basis of their molecular weights as determined by SDS-PAGE, reaction of identity in immunodiffusion assay and similar antigen binding activities as shown by ELISA.

TL;DR: It is demonstrated that CD11b is expressed at maximal levels on arthritic synovial fluid neutrophils and CD14 +ve cells, and at increased but submaximal levels on peripheral blood neutrophil and monocytes of patients recovering from sepsis.

TL;DR: Results have shown that cytokine-specific IL-2, IL-4 and IL-6 ELISPOT assays have now been established and will allow analysis of the frequency of cytokin-secreting cells at the single cell level.

TL;DR: Results demonstrate that particle bombardment is an effective means for gene transfer and provides an attractive approach for rapid, quantitative analysis of transgene expression in various leukocyte primary culture systems.

TL;DR: The interaction between human heart myoglobin and ten specific monoclonal antibodies was investigated with a new biosensor technology, real time biospecific interaction analysis (RT BIA), using surface plasmon resonance.

TL;DR: A plasmid vector is described that drives the expression of foreign cDNAs in transgenic mice, according to the dictates of an MHC class II gene promoter, and has often obtained mRNA and protein synthesis with a tissue and cell-type specificity indistinguishable from that of the endogenous MHCclass II genes.