Showing papers in "Journal of Microbiological Methods in 1990"

TL;DR: A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid.

TL;DR: In the present paper, methods for the analysis of fatty acids liberated from LPS (or lipid A) are described and described.

TL;DR: A significant reduction in mRNA degradation was achieved by a drastic reduction in time for cell separation and employing a phenol-choloroform-SDS mixture of RNA extraction.

TL;DR: Results suggest that UV-absorbing bacterial taxonomic markers can be selectively excited to give rise to characteristics resonance Raman bacterial spectral fingerprints which have the potential to be used as the basis for methods of rapid identification.

TL;DR: It is concluded that the assays are good alternatives to more conventional and labour-intensive methods of measuring total microbial activity, and that the INT assay is best-suited for measuring cell density whereas the FDA assay is better for estimates of decomposer activity.

TL;DR: It is shown here that genomic and plasmid DNA sequences can be amplified by the PCR directly from single colonies of both Escherichia coli and Bacillus subtilis without any purification of the template.

TL;DR: In this article, a liquid-drying method using activated charcoal as a carrier material for the long-term preservation of sensitive microorganisms is described, where drops of cell suspensions were dried under mild vacuum on thin discs of activated charcoal containig skim milk and effective protective agents.

TL;DR: The rates of [methyl-3H]thymidine incorporation into DNA were used to determine bacterial growth rates in seagrass sediments and the distribution of radioactivity in macromolecules extracted from the sediment was determined by hydrolytic enzymes that were specific for DNase and protein.

TL;DR: In this paper, the overall properties of the cell surface of Streptococcus thermophilus and Leuconostoc mesenteroides, two fouling microorganisms from dairy industry, were determined by a variety of physico-chemical methods.

TL;DR: In this paper, different methods were tested for the preparation of immobilizing material and bacteria entrapped in it for scanning electron microscopy (SEM), and the internal and external structure of the beads and appearance of the immobilized bacteria were compared different processing techniques for SEM.

TL;DR: Irrespective of the method used, the grouping was consistent with identifications based on conidium morphology, demonstrating that statistical evaluation of quantitative data are useful for fungal chemotaxonomy.

TL;DR: This method for biochemical fingerprinting is reproducible, highly discriminating and very simple to use, suitable for epidemiological and ecological investigations and may also be used for studies involving large numbers of isolates.

TL;DR: Activated charcoal protected phototrophically grown cultures against photooxidation by absorbing light and O before freeze-drying, and several anaerobic purple nonsulfur bacteria, Chromatiaceae, Ectothiorhodosporaceae and green sulfur bacteria were successfully preserved.

TL;DR: This study is the first demonstration of the use of in situ hybridization for yeast detection by flow cytomertry and the yeast-specific signal was not eliminated after posthybridization RNase treatment, and it completely disappeared when cells were treated with RNase before hybirdization, confirming the rRNA was the target.

TL;DR: REF proved to be a specific and reproducible typing method which could be applicable to molecular epidemiology of genetically diverse group of coagulase-negative staphylococci.

TL;DR: Simple pulse-shape parameters (duration, height and integral) were extracted by analogue circuitry and the relationship between these parameters analysed digitaly provided low-resolution morphological information at a throughput rate up to 1000 cells·s −1 : such information is particularly useful in the flow analysis of larger algal cells and colonial species.

TL;DR: Chelex 100 resin when added to tryptone soy and brain heart infusion broths and stirred under controlled conditions, removed iron and other cations from complex media rapidly and efficiently.

TL;DR: Growth in the ascorbate medium allowed for formation of the typical fermentation products of this strain as well as redox balances which were similar to those obtained with a medium containing cysteine.

TL;DR: Fluorescence in tube and on agar has been determined as the factor which allows the detection of E. coli presence in the three sample types with high accuracy and the incorporation of MUG to the selective broth in the confirmatory test has been shown to avoid false-positive results due to shellfish tissue β-glucuronidase activity.

TL;DR: A nondenaturing-gel activity assay for the detection of azoreductases from anaerobic bacteria is described and suggests that a single protein may be involved in the reduction of azo dye compounds and Nitro Blue Tetrazolium.

TL;DR: The usefulness of the system for investigations of the kinetics of growth on, and utilization of, solid substrates is illustrated by results obtained with one strain each of Ruminococcus flavefaciens and Fibrobacter (Bacteroides) succinogenes, grown on ball-milled filter paper cellulose.

TL;DR: Lysostaphin was purified from a commercially available specimen by using a dye ligand affinity high-pressure liquid chromatography (HPLC) procedure, which is simple and rapid, with high recovery and useful for preparing analytical grade-lysostaph in order to investigate the cell-wall proteins of staphylococcus species.

TL;DR: In this paper, the authors compared the salmonella detection and enumeration from seawater samples with low and moderate pollution levels using 10 μm·ml − of sodium novobiocin as preenrichment medium.

TL;DR: The assay described offers advantages over existing techniques and is simple, quick and suitable for use in screening programmes or in studies on enzyme kinetics.

TL;DR: The combination of three solid media yielded 73 of 81 clinically significant isolates whereas BACTEC detected 62 of 81 compared to MB Check broth which yielded 78 of 81.

TL;DR: In this paper, a high-pressure liquid ion chromatography method using a borate/gluconate eluent to determine sulfate present in sewage sludge is described.

TL;DR: The results demonstrate that the use of commercially available identification systems could be restricted to nonfluorescent colonies to save time and costs.

TL;DR: 17 strains of Porphyromonas and 12 other species of Bacteroides were studied for their coaggregation with Actinomyces viscosus and P. gingivalis was found to coaggregate with A. visCosus.

TL;DR: A dimeric derivative of ethidium (ethidium homodimer) was used to fluorometrically assay for in situ levels of cellular DNA and RNA in artificial seawater samples because of the very high binding affinity for both RNA and DNA.

TL;DR: Microcalorimetry may be used to detect genetically modified bacterial strains within reasonable time, sensitivity and accuracy limits and beyond, the technique may also be used for an evaluation of the fitness of unknown microorganism-plasmid associations.