Showing papers in "Systematic and Applied Microbiology in 2011"

TL;DR: In this article, a polyphasic approach based on molecular and phenotypic methods was used to clarify the taxonomy of representative Phl(+) Plt(+) strains isolated from tobacco, cotton or wheat on different continents.

TL;DR: An over-view of the principles of MALDI-TOF MS and WC-MS is presented, highlighting the characteristics of the technology that allow its utilization for systematic microbiology.

TL;DR: Symbiotic variant (symbiovar) is proposed as a parallel term to pathovar in pathogenic bacteria, distinguished by the differences in host range and supported by symbiotic gene sequence information.

TL;DR: A standard protocol was developed to generate MALDI-TOF mass spectra and the spectra were successfully used for species identification within the genera Leuconostoc, Fructobacillus, and Lactococcus.

TL;DR: Diversity of RAPD types found in food versus human samples suggests the importance of host factors in colonization and individual host specificity, and support the hypothesis that there is a vertical transfer of intestinal LAB from the mother's gut to her milk and through the milk to the infant's gut.

TL;DR: The state-of-the-art taxonomic methods for the classification and identification of the Burkholderia cepacia complex are presented in this article. But, they do not consider the use of DNA-DNA hybridization for species level demarcation.

TL;DR: The Elsevier Publisher, Max Planck Society, and the Spanish Ministry of Science and Innovation (Projects Consolider Ingenio 2010 CE-CSD2007-0005 and CGL2009_12651-C02-02, both co-financed with FEDER funding) for their economic support.

TL;DR: The genetic and phenotypic properties of strains Re1(T), Re2, T1 and T2 suggest classification as representatives of a novel species of the genus Tepidanaerobacter; the name Tepicutes-Clostridia acetatoxydans sp.

TL;DR: The outcome of the isolation campaign and screening of reference strain set suggest that bacilli may be potential contributors to denitrification in terrestrial and possibly other ecosystems.

TL;DR: Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, a new species of a novel genus represented by strain F2-233(T) is described and the name Gaiella occulta gen. nov. is proposed for this organism which represents a novel family named Gaiellaceae fam.nov.

TL;DR: The intra- and inter-species phylogenetic relationships of 115 strains representing all Aeromonas species described to date were investigated by MLPA and the phylogenies obtained were consistent with each other, and clustering agreed with the aeromonas taxonomy recognized to date.

TL;DR: In this paper, the abundance and diversity of AOA and ammonia-oxidizing beta-proteobacteria (AOB) in estuarine sediments in Hong Kong for two seasons using the ammonia monooxygenase A subunit gene (amoA) as molecular biomarker.

TL;DR: The present study confirmed the diversity of lactic acid bacteria species inhabiting silages and showed that the differing natural populations of LAB on these silages might influence fermentation quality, enabling future research on the relationship between LAB species and silage fermentation quality.

TL;DR: In this paper, the authors performed sequencing of 16S rRNA genes and internal transcribed spacer (ITS) regions, together with detailed morphological analysis of hand-picked individuals of novel non-filamentous as well as known filamentous large sulfur bacteria.

TL;DR: In this paper, the 16S rRNA gene was used as a molecular marker to identify the bacterial community in the cecum of wild and captive tetraonid capercaillies.

TL;DR: In this paper, the authors assessed the suitability of MALDI-TOF MS for the identification of S. intermedius, S. pseudintermedius and S. delphini.

TL;DR: In this article, the authors determined the phylogenetic composition of flow cytometrically sorted HNA and LNA populations, collected at six stations along a transect across three oceanic provinces from Iceland to the Azores.

TL;DR: In this article, the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of gram positive anaerobic cocci (GPAC) was evaluated.

TL;DR: In this article, three mussel isolates that were Gram-negative slightly curved rods, non-spore forming, showed a new 16S rDNA-RFLP pattern with a specific identification method for the species of this genus.

TL;DR: It is proposed that Sulfurihydrogenibium spp.

TL;DR: Using 453 Legionella isolates previously characterized by genotyping, it was possible to create species-specific SuperSpectra, using appropriate sets of spectral masses, allowing unambiguous differentiation and identification of the most frequently isolated Legionella species.

TL;DR: Overall this study showed that the high genetic diversity derived equally from recombination and point mutation accumulation, however, X. campestris remains a species with a clonal evolution driven by a differential adaptation to cruciferous hosts.

TL;DR: Three Gram-negative bacterial strains isolated from the biofilter of a recirculating marine aquaculture are proposed as new species of two novel genera, Defluviimonas denitrificans gen. nov.

TL;DR: The authors used multilocus sequence analysis to identify 36 Vibrio harveyi-like isolates obtained from a wide range of sources in Australia and to re-evaluate the identity of important pathogens.

TL;DR: In this paper, the authors investigated 62 Enterococcus strains isolated from clinical, food and environmental origins towards a rationale for safety evaluation of strains in food or probiotic applications, all isolates were characterised with respect to the presence of the virulence determinants fsrB, sprE, gelE, ace, efaAfs/fm, as, esp, cob and the cytolysin operon.

TL;DR: In this paper, 19 bacteria isolates recovered from shellfish samples (mussels and oysters) showed a new and specific 16S rDNA-RFLP pattern with an Arcobacter identification method designed to recognize all species described up to 2008.

TL;DR: This study compared the cluster analysis of 88 strains of species of the S. bovis/equinus complex by sequence analysis of the manganese-dependent superoxide dismutase gene (sodA) and by Matrix Assorption/Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS).

TL;DR: Twenty-three Bradyrhizobium strains isolated in Poland from root nodules of lupin species, and pink serradella strains, revealed that these species are the two dominant groups in soils of central Europe, in the nodule of l upin and serradeella plants.

TL;DR: Based on phylogenetic, genotypic and phenotypic characteristics, strain GNS-1(T) is proposed to be assigned to a novel species of a novel genus within the class Anaerolineae (subphylum I), phylum "Chloroflexi", Thermanaerothrix daxensis gen. nov.

TL;DR: A total of 51 LBSs and 28 LBS curvatus strains from different origins were screened for their potential to produce biogenic amines (BAs), and for their diversity of peptidolytic systems and specific aminotransferases (AraT, BcaT) that initiate amino acid conversion to volatiles relevant for aroma formation in meat products as mentioned in this paper.