Journal ArticleDOI
24-Epibrassinolide ameliorates the effects of boron toxicity on Arabidopsis thaliana (L.) Heynh by activating an antioxidant system and decreasing boron accumulation
Reads0
Chats0
TLDR
This study showed that the exogenous application of EBL ameliorates the toxic effects of B in a model plant by improving the plant’s antioxidant system and decreasing B accumulation.Abstract:
Brassinosteroids (BRs) play a significant role in alleviating the negative effects of various environmental stresses and in promoting the growth and development of plants. In this study, we investigated the effects of 24-epibrassinolide (EBL) on the growth, boron (B) accumulation and activation of the antioxidant system of Arabidopsis thaliana (L.) Heynh exposed to high concentrations of boric acid (BA). A. thaliana plants were grown in a hydroponic culture, and after 4 weeks, the plants were transferred to media containing either 0.80 or 1.60 mM BA. Following BA treatment, 0.01 and 1 µM EBL was sprayed on the entire foliar region of the seedlings. B toxicity induced oxidative stress and considerably inhibited the growth of the plants. The spraying of EBL on the B-treated plants resulted in increases in growth (both fresh and dry shoot mass, silique number, length and mass) and pigment content (total chlorophyll and carotenoids). Excessive B levels increased the activities of antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase, and increased the proline content in leaves of plants. However, treatment of the B-stressed plants with EBL further enhanced the activities of the antioxidant enzymes and increased the content of proline. The high level of lipid peroxidation in plants observed during exposure to a higher level of BA was decreased following EBL treatment. Thus, this study showed that the exogenous application of EBL ameliorates the toxic effects of B in a model plant by improving the plant’s antioxidant system and decreasing B accumulation. To our knowledge, this is the one of the first studies to examine the effect of BR in plants subjected to B toxicity.read more
Citations
More filters
Journal ArticleDOI
Gibberellic acid-induced generation of hydrogen sulfide alleviates boron toxicity in tomato (Solanum lycopersicum L.) plants.
Cengiz Kaya,Ali Sarioğlu,Muhammad Ashraf,Mohammed Nasser Alyemeni,Parvaiz Ahmad,Parvaiz Ahmad +5 more
TL;DR: GA-induced BT tolerance was enhanced by the supplementation of sodium hydrosulfide and addition of HT reversed the beneficial effect of GA on oxidative stress and antioxidant defence system by reducing the endogenous H2S without changing L-DES activity, suggesting that H1S participates in GA-induced tolerance to BT of tomato plants.
Journal ArticleDOI
Alleviation of boron toxicity in plants: Mechanisms and approaches
TL;DR: Boron (B) is an essential element for higher plants, while it becomes toxic when present in excessive concentrations as discussed by the authors, and many approaches have been developed to alleviate B toxicity in plants.
Journal ArticleDOI
Silicon is dependent on hydrogen sulphide to improve boron toxicity tolerance in pepper plants by regulating the AsA-GSH cycle and glyoxalase system.
Cengiz Kaya,Muhammad Ashraf,Asma A. Al-Huqail,Moneerah A. Alqahtani,Parvaiz Ahmad,Parvaiz Ahmad +5 more
TL;DR: Silicon stimulated growth, proline content and activities of various antioxidant biomolecules and enzymes, leaf Ca2+, K+ and N, endogenous H2S and L-DES activity, but reduced H2O2 and MDA contents, membrane leakage and leaf B, and Silicon-induced B tolerance was further enhanced by 0.2 mM NaHS, a H 2S donor.
Journal ArticleDOI
Effect of 24-epibrassinolide on reactive oxygen species and antioxidative defense systems in tall fescue plants under lead stress
Woxiu Zhong,Xie Chengcheng,Dan Hu,Pu Siyi,Xi Xiong,Jun Ma,Lingxia Sun,Huang Zhuo,Mingyan Jiang,Xi Li +9 more
TL;DR: Foliar applications of 24-epibrassinolide enhanced the lead recovery rate of tall fescue plants, proving its potential role in phytoremediation for soil contaminated with heavy metals such as lead.
Journal ArticleDOI
Effects of boron toxicity on growth, oxidative damage, antioxidant enzymes and essential oil fingerprinting in Mentha arvensis and Cymbopogon flexuosus
TL;DR: In this article, the effects of high Boron concentrations (2.5, 5, 10, 20 and 30 mg/kg) were elucidated on two important essential oil-bearing plants; Mentha arvensis and Cymbopogon flexuosus which are aromatic and antimicrobial herbs having well-known medicinal values.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris
TL;DR: Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented.
Journal ArticleDOI
Rapid determination of free proline for water-stress studies
TL;DR: In this article, a simple colorimetric determination of proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material was presented.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.
Journal ArticleDOI
Oxidative stress, antioxidants and stress tolerance
TL;DR: Key steps of the signal transduction pathway that senses ROIs in plants have been identified and raise several intriguing questions about the relationships between ROI signaling, ROI stress and the production and scavenging ofROIs in the different cellular compartments.