A B-lectin receptor kinase gene conferring rice blast resistance.
Xuewei Chen,Junjun Shang,De-xi Chen,Cailin Lei,Yan Zou,Wenxue Zhai,Guozhen Liu,Jichen Xu,Zhongzhuan Ling,Gang Cao,Bingtian Ma,Yuping Wang,Xianfeng Zhao,Shigui Li,Lihuang Zhu +14 more
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TLDR
The dominant resistance gene Pi-d2, present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15, and represents a new class of plant resistance genes.Abstract:
Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 [previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes.read more
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References
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TL;DR: The current knowledge of recognition-dependent disease resistance in plants is reviewed, and a few crucial concepts are included to compare and contrast plant innate immunity with that more commonly associated with animals.
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FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis.
TL;DR: The identification of a new locus, FLS2, is described, which is ubiquitously expressed and encodes a putative receptor kinase and shares structural and functional homologies with known plant resistance genes and with components involved in the innate immune system of mammals and insects.
Journal ArticleDOI
A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21
Wen-Yuan Song,Guo-Liang Wang,Lili Chen,Han Suk Kim,Liya Pi,Tom Holsten,John M. Gardner,Bei Wang,Wen Xue Zhai,Li Huang Zhu,Claude M. Fauquet,Pamela C. Ronald +11 more
TL;DR: The rice Xa21 gene, which confers resistance to Xanthomonas oryzae pv. race 6, was isolated by positional cloning and the sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response.
A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. - eScholarship
W Y Song,G L Wang,L L Chen,H S Kim,L Y Pi,T Holsten,J Gardner,B Wang,W X Zhai,L H Zhu,C Fauquet,P Ronald +11 more
TL;DR: The sequence of the predicted protein, which carries both a leucine-rich repeat motif and a serine-threonine kinase-like domain, suggests a role in cell surface recognition of a pathogen ligand and subsequent activation of an intracellular defense response.