A Brassinosteroid-Insensitive Mutant in Arabidopsis thaliana Exhibits Multiple Defects in Growth and Development
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TLDR
Genetic analysis suggests that the Bri1 phenotype is caused by a recessive mutation in a single gene with pleiotropic effects that maps 1.6 centimorgans from the cleaved, amplified, polymorphic sequence marker DHS1 on the bottom of chromosome IV, suggesting that the BRI1 gene may play a critical role in brassinosteroid perception or signal transduction.Abstract:
Brassinosteroids are widely distributed plant compounds that modulate cell elongation and division, but little is known about the mechanism of action of these plant growth regulators. To investigate brassinosteroids as signals influencing plant growth and development, we identified a brassinosteroid-insensitive mutant in Arabidopsis thaliana (L.) Henyh. ecotype Columbia. The mutant, termed bri1, did not respond to brassinosteroids in hypocotyl elongation and primary root inhibition assays, but it did retain sensitivity to auxins, cytokinins, ethylene, abscisic acid, and gibberellins. The bri1 mutant showed multiple deficiencies in developmental pathways that could not be rescued by brassinosteroid treatment including a severely dwarfed stature; dark green, thickened leaves; males sterility; reduced apical dominance; and de-etiolation of dark-grown seedlings. Genetic analysis suggests that the Bri1 phenotype is caused by a recessive mutation in a single gene with pleiotropic effects that maps 1.6 centimorgans from the cleaved, amplified, polymorphic sequence marker DHS1 on the bottom of chromosome IV. The multiple and dramatic effects of mutation of the BRI1 locus on development suggests that the BRI1 gene may play a critical role in brassinosteroid perception or signal transduction.read more
Citations
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A Putative Leucine-Rich Repeat Receptor Kinase Involved in Brassinosteroid Signal Transduction
Jianming Li,Joanne Chory +1 more
TL;DR: The identification of 18 Arabidopsis dwarf mutants that are unable to respond to exogenously added brassinosteroid, a phenotype that might be expected for brass inosteroid signaling mutants.
Journal ArticleDOI
BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development
TL;DR: This review examines the microchemical and molecular genetic analyses that have provided convincing evidence for an essential role of BRs in diverse developmental programs, including cell expansion, vascular differentiation, etiolation, and reproductive development.
Journal ArticleDOI
BAK1, an Arabidopsis LRR Receptor-like Protein Kinase, Interacts with BRI1 and Modulates Brassinosteroid Signaling
TL;DR: Results indicate BAK1 is a component of BR signaling, and Expression of a dominant-negative mutant allele of B AK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles.
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Abscisic acid signal transduction
Jeffrey Leung,Jérôme Giraudat +1 more
TL;DR: Substantial evidence points to the importance of reversible protein phosphorylation and modifications of cytosolic calcium levels and pH as intermediates in ABA signal transduction.
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BRI1/BAK1, a Receptor Kinase Pair Mediating Brassinosteroid Signaling
Kyoung Hee Nam,Jianming Li +1 more
TL;DR: It is proposed that BAK1 and BRI1 function together to mediate plant steroid signaling.
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