Journal ArticleDOI
A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae.
Elena N. Elpidina,T. A. Tsybina,Yakov E. Dunaevsky,Mikhail A. Belozersky,D. P. Zhuzhikov,Brenda Oppert +5 more
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TLDR
A chymotrypsin-like proteinase was isolated from the posterior midgut of larvae of the yellow mealworm, Tenebrio molitor, by ion-exchange and gel filtration chromatography and found that the best substrate was SucAAPFpNA, with k(cat app) 36.5 s(-1) and K(m) 1.59 mM.About:
This article is published in Biochimie.The article was published on 2005-08-01. It has received 61 citations till now. The article focuses on the topics: Kunitz STI protease inhibitor & Chymotrypsin.read more
Citations
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Journal ArticleDOI
Extraction and characterisation of protein fractions from five insect species
Liya Yi,Catriona M.M. Lakemond,Leonard M.C. Sagis,Verena Eisner-Schadler,Arnold van Huis,Martinus A.J.S. van Boekel +5 more
TL;DR: The insect species studied have potential to be used in foods due to: (1) absolute protein levels; (2) protein quality; (3) ability to form gels.
Journal ArticleDOI
Recovery and techno-functionality of flours and proteins from two edible insect species: Meal worm (Tenebrio molitor) and black soldier fly (Hermetia illucens) larvae.
TL;DR: This work investigates the protein extractability and techno-functionality of insect flour fractions recovered from Tenebrio molitor and Hermetia illucens and isolates proteins from the flours by optimizing protein solubility, composition and structure.
Book ChapterDOI
Biochemistry and Molecular Biology of Digestion
Walter R. Terra,Clélia Ferreira +1 more
TL;DR: The aim of this chapter is to establish uniform parameters for studying insect digestive enzymes, providing an overview of the biochemistry of insect digestion, and discusses factors affecting digestive enzymes in vivo.
Journal ArticleDOI
Diversity of digestive proteinases in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae.
K.S. Vinokurov,Elena N. Elpidina,Brenda Oppert,S. Prabhakar,D. P. Zhuzhikov,Yakov E. Dunaevsky,Mikhail A. Belozersky +6 more
TL;DR: Data support a complex system of protein digestion, and the correlation of proteinase activity and pH indicates a physiological mechanism of enzyme regulation in the gut.
Journal ArticleDOI
Molecular cloning and expression analysis of chymotrypsin-like serine protease from the Chinese shrimp, Fenneropenaeus chinensis.
TL;DR: A new member of the serine protease (SP) chymotrypsin (designated Fc-chy) was isolated from the hepatopancreas of Chinese shrimp Fenneropenaeus chinensis and showed high hydrolytic activity toward the substrate N-succinyl-ala-ala -ala-pro-phe p-nitroanilide (AAPF), and its activity was inhibited by Kazal-type SP inhibitor from Chinese shrimp.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The preparation and properties of two new chromogenic substrates of trypsin.
TL;DR: Preliminary studies indicate that benzoyl dl -arginine p -nitroanilide hydrochloride is also hydrolyzed by papain, and that of l -LPA is in a more alkaline region than normally found for trypsin substrates.
Book
Handbook of proteolytic enzymes
TL;DR: In this paper, Serine Peptidases with a Ser/Lys Catalytic Dyad (SC) are described, as well as their relation to the Nodavirus Coat Protein.
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Walter R. Terra,Clélia Ferreira +1 more