Journal ArticleDOI
A comparative study of submicron particle sizing platforms: Accuracy, precision and resolution analysis of polydisperse particle size distributions
Will Anderson,Darby Kozak,Victoria A. Coleman,Åsa K. Jämting,Matt Trau +4 more
- Vol. 405, pp 322-330
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TLDR
The large variation in the PSDs obtained from these four, fundamentally different platforms, indicates that great care must still be taken in the analysis of samples known to have complex PSDs.Abstract:
The particle size distribution (PSD) of a polydisperse or multimodal system can often be difficult to obtain due to the inherent limitations in established measurement techniques. For this reason, the resolution, accuracy and precision of three new and one established, commercially available and fundamentally different particle size analysis platforms were compared by measuring both individual and a mixed sample of monodisperse, sub-micron (220, 330, and 410 nm - nominal modal size) polystyrene particles. The platforms compared were the qNano Tunable Resistive Pulse Sensor, Nanosight LM10 Particle Tracking Analysis System, the CPS Instruments's UHR24000 Disc Centrifuge, and the routinely used Malvern Zetasizer Nano ZS Dynamic Light Scattering system. All measurements were subjected to a peak detection algorithm so that the detected particle populations could be compared to 'reference' Transmission Electron Microscope measurements of the individual particle samples. Only the Tunable Resistive Pulse Sensor and Disc Centrifuge platforms provided the resolution required to resolve all three particle populations present in the mixed 'multimodal' particle sample. In contrast, the light scattering based Particle Tracking Analysis and Dynamic Light Scattering platforms were only able to detect a single population of particles corresponding to either the largest (410 nm) or smallest (220 nm) particles in the multimodal sample, respectively. When the particle sets were measured separately (monomodal) each platform was able to resolve and accurately obtain a mean particle size within 10% of the Transmission Electron Microscope reference values. However, the broadness of the PSD measured in the monomodal samples deviated greatly, with coefficients of variation being ~2-6-fold larger than the TEM measurements across all four platforms. The large variation in the PSDs obtained from these four, fundamentally different platforms, indicates that great care must still be taken in the analysis of samples known to have complex PSDs. All of the platforms were found to have high precision, i.e. they gave rise to less than 5% variance in PSD shape descriptors over the replicate measurements.read more
Citations
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Journal ArticleDOI
Making Sense of Brownian Motion: Colloid Characterization by Dynamic Light Scattering
TL;DR: This historical review of dynamic light scattering discusses the basic principle, data analysis, and important precautions to be taken while analyzing colloids using DLS in a manner that can be understood by a beginner.
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Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications
Kenneth W. Witwer,Bas W.M. van Balkom,Stefania Bruno,Andre Choo,Massimo Dominici,Mario Gimona,Andrew F. Hill,Dominique P.V. de Kleijn,M. Koh,M. Koh,Ruenn Chai Lai,S. Alex Mitsialis,Luis A. Ortiz,Eva Rohde,Takashi Asada,Wei Seong Toh,Daniel J. Weiss,Lei Zheng,Bernd Giebel,Sai Kiang Lim,Sai Kiang Lim +20 more
TL;DR: MSC-sEVs should be defined by quantifiable metrics to identify the cellular origin of the sEVs in a preparation, presence of lipid-membrane vesicles, and the degree of physical and biochemical integrity of the vesicle.
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The Methods of Choice for Extracellular Vesicles (EVs) Characterization.
Rafał Szatanek,Monika Baj-Krzyworzeka,Jakub Zimoch,Małgorzata Lekka,Maciej Siedlar,Jarek Baran +5 more
TL;DR: In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
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Challenges and opportunities in exosome research—Perspectives from biology, engineering, and cancer therapy
Xia Li,Alexander L. Corbett,Erfan Taatizadeh,Nishat Tasnim,Jonathan P. Little,Cathie Garnis,Mads Daugaard,Emma S. Tomlinson Guns,Mina Hoorfar,Isaac T. S. Li +9 more
TL;DR: The current knowledge in exosome biogenesis, their roles in disease progression, and therapeutic applications and opportunities in bioengineering are summarized and the established and emerging technological developments inExosome isolation and characterization are highlighted.
Journal ArticleDOI
A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles
TL;DR: In this article, the accuracy of dynamic light scattering (DLS) measurements compared with transmission electron microscopy (TEM) studies for characterization of size distributions of ceramic nanoparticles was compared.
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Journal ArticleDOI
Counting and Sizing of Submicron Particles by the Resistive Pulse Technique
R. W. DeBlois,C. P. Bean +1 more
TL;DR: In this article, the resistive (Coulter) method of counting and sizing particles in a conducting fluid has been extended to polystyrene spheres 900 A in diameter, with a present detection limit near 600 A, through the use of individual submicron pores etched in irradiated plastic sheet.