A comparison of procedures to determine free fatty acids in rat heart.
J. K. G. Kramer,H. W. Hulan +1 more
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TLDR
The high levels of free fatty acid and diglyceride reported in the cardiac lipids of rats fed rapeseed oil suggest that extensive autolysis occurs during homogenization with a rotating blade-type homogenizer.About:
This article is published in Journal of Lipid Research.The article was published on 1978-01-01 and is currently open access. It has received 101 citations till now. The article focuses on the topics: Fatty acid & Homogenization (biology).read more
Citations
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Uptake and tissue content of fatty acids in dog myocardium under normoxic and ischemic conditions.
TL;DR: Enhanced lipolysis of endogenous lipids or reduced combustion may be held responsible for the accumulation of NEFA in ischemic tissue, since arachidonic and linoleic acids showed the highest relative increase and lipolytic of endogenous phospholipids, rich in these fatty acids, seems to be reasonable.
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Mitochondrial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol.
L G Lange,B E Sobel +1 more
TL;DR: Operation of this shuttle as a result of ethanol ingestion and subsequent accumulation of fatty acid ethyl esters may account for the impaired mitochondrial function and inefficient energy production associated with toxic effects of ethanol on the heart.
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Distributions of conjugated linoleic acid (CLA) isomers in tissue lipid classes of pigs fed a commercial CLA mixture determined by gas chromatography and silver ion-high-performance liquid chromatography.
John K. G. Kramer,Najibullah Sehat,Michael E. R. Dugan,Magdi M. Mossoba,Martin P. Yurawecz,John A. G. Roach,Klaus Eulitz,Jennifer L. Aalhus,Allan L. Schaefer,Youh Ku +9 more
TL;DR: The preferential accumulation of the 11 cis,13 trans-18∶2 into cardiac lipids, and in particular the major phospholipid in the inner mitochondrial membrane, DPG, in both heart and liver, appears unique and may be of concern.
Journal ArticleDOI
Analysis of conjugated linoleic acid and trans 18:1 isomers in synthetic and animal products
John K. G. Kramer,Cristina Cruz-Hernandez,Zeyuan Deng,Jianqiang Zhou,Gerhard Jahreis,Michael E. R. Dugan +5 more
TL;DR: Improved techniques are used to evaluate the CLA profile in pork products from pigs fed different commercial CLA mixtures, and are essential to evaluate dairy fats for their CLA content, to design experimental diets to increase the amount of CLA in dairy fats, and to determine theCLA profile in these CLA-enriched dairy fats.
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Insect lipid profile: aqueous versus organic solvent-based extraction methods
Daylan A. Tzompa-Sosa,Liya Yi,Hein J.F. van Valenberg,Martinus A.J.S. van Boekel,Catriona M.M. Lakemond +4 more
TL;DR: In this paper, the influence of extraction methods on chemical characteristics of insect lipids was investigated in view of future expected industrial bio-fractionation of insects, and the major findings on lipid chemical characterization were the following: (1) Tenebrio molitor had the highest lipid content around 13%; (2) the highest yield was obtained using Folch extraction and the lowest yield using the aqueous method (from 19 to 60% related to the lipid recovery of folch extraction).
References
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Book
Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids
TL;DR: The second edition of this book updates procedures and instrumentation but also enlarges on the theoretical basis of the book where appropriate, and adds chemical structures of new lipid classes and components.
Journal ArticleDOI
Analysis of Long-Chain Fatty Acids by Gas-Liquid Chromatography
Journal ArticleDOI
Determination of lipid classes by a gas-chromatographic procedure
TL;DR: In this article, gas chromatography of the component fatty acids with added internal standard can be used to determine a wide variety of neutral and polar lipids, after separation by thin-layer chromatography, if precautions are taken to minimize losses.
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A simple method for the isolation and purification of total lipides from animal tissues.
Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol
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