Journal ArticleDOI
A direct correlation between hyperthermia-induced membrane blebbing and survival in synchronous G1 CHO cells.
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TLDR
It was demonstrated that cells with blebs larger than 50% of the cell diameter did not survive to form colonies and all subcellular organelles, save the ribosomes, were absent from the membrane blebs.Abstract:
Heating synchronous G1 cells at 45.5 degrees C for 3-20 min induced varying degrees of membrane blebbing ranging from nonblebbed cells indistinguishable from control cells to those with blebs larger than the cell itself. Both the proportion of cells exhibiting blebbing and the mean diameter of the blebs increased with heating duration. Scoring individual cells for both blebbing and colony formation demonstrated that cells with blebs larger than 50% of the cell diameter did not survive to form colonies. Electron microscopy showed that all subcellular organelles, save the ribosomes, were absent from the membrane blebs. Freeze fracture replicas revealed no changes in membrane ultrastructure, except on some 15% of the blebs that contained bald patches devoid of membrane particles.read more
Citations
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Journal ArticleDOI
The cellular and molecular basis of hyperthermia.
Bert Hildebrandt,Peter Wust,Olaf Ahlers,Annette Dieing,Geetha Sreenivasa,Thoralf Kerner,Roland Felix,Hanno Riess +7 more
TL;DR: The direct cytotoxic effect of heat, heat-induced alterations of the tumor microenvironment, synergism of heat in conjunction with radiation and drugs, as well as, the presumed cellular effects of hyperthermia including the expression of heat-shock proteins (HSP), induction and regulation of apoptosis, signal transduction, and modulation of drug resistance byhyperthermia are discussed.
Journal ArticleDOI
Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice.
Erin B. Dickerson,Erik C. Dreaden,Xiaohua Huang,Ivan H. El-Sayed,Hunghao Chu,Sujatha Pushpanketh,John F. McDonald,Mostafa A. El-Sayed +7 more
TL;DR: The present work demonstrates the feasibility of in vivo PPTT treatment of deep-tissue malignancies using easily-prepared plasmonic gold nanorods and a small, portable, inexpensive near-infrared (NIR) laser.
Journal ArticleDOI
Cellular effects of hyperthermia: relevance to the minimum dose for thermal damage.
TL;DR: The high activation energy of cell killing and other responses to hyperthermia suggest that protein denaturation is the rate-limiting step, which leads to the aggregation of both denatured and native protein with multiple effects on cellular function.
Journal ArticleDOI
Viability measurements in mammalian cell systems.
John A. Cook,James Mitchell +1 more
TL;DR: The purpose of this review is to examine the merits of the major groups of currently available viability assays as they apply to mammalian cell systems and to show that while some assays may be clearly superior in measuring a final endpoint, technical and practical considerations may limit their usefulness.
Journal ArticleDOI
Mechanism of cell death induced by magnetic hyperthermia with nanoparticles of γ-MnxFe2–xO3 synthesized by a single step process
TL;DR: Both the magnetic and XRD data support the substitution of Mn3+ ions at the tetrahedral site of γ-Fe2O3 and suggest that the suspension of these particles may be evaluated for magnetic hyperthermia treatment of cancer.
References
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Journal ArticleDOI
Cross-linker system between neurofilaments, microtubules and membranous organelles in frog axons revealed by the quick-freeze, deep-etching method
TL;DR: The elaborate cross-connections among membranous organelles, microtubules, and neurofilaments were demonstrated in unifixed axons by the quick-freeze, deep-etch, and rotary-shadowing method and it is concluded that the cross- connections are real structures while the granular material is composed of soluble material, probably protein in nature.
Journal ArticleDOI
Variation in Sensitivity to Heat Shock during the Cell-cycle of Chinese Hamster Cells in Vitro
Arthur Westra,W.C. Dewey +1 more
TL;DR: Heating of synchronous cells, obtained by the selective removal of mitotic cells from an asynchronous population, revealed that the mitotic and S phases were the most sensitive, primarily indicated by the smaller shoulders on the survival curves compared with the curve for cells heated in the resistant G1 phase.
Journal ArticleDOI
A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.
TL;DR: The results suggest an attachment of polyribosomes to the cytoskeleton via mRNA, which is similar to that in intact cells.
Journal ArticleDOI
Regulation of protein synthesis in HeLa cells: Translation at elevated temperatures☆
William McCormick,Sheldon Penman +1 more
TL;DR: An early function in translation is shown to be rate limiting at the elevated temperature and is the cause of polyribosome disaggregation and an RNA factor is produced which appears to promote the association of ribosomes with messenger RNA.
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