A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization
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TLDR
A dual-modality system, incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM), that is capable of simultaneously characterizing the 3-D tissue morphology and its biochemical composition is developed.Abstract:
Most pathological conditions elicit changes in the tissue optical response that may be interrogated by one or more optical imaging modalities. Any single modality typically only furnishes an incomplete picture of the tissue optical response, hence an approach that integrates complementary optical imaging modalities is needed for a more comprehensive non-destructive and minimally-invasive tissue characterization. We have developed a dual-modality system, incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM), that is capable of simultaneously characterizing the 3-D tissue morphology and its biochemical composition. The Fourier domain OCT subsystem, at an 830 nm center wavelength, provided high-resolution morphological volumetric tissue images with an axial and lateral resolution of 7.3 and 13.4 µm, respectively. The multispectral FLIM subsystem, based on a direct pulse-recording approach (upon 355 nm laser excitation), provided two-dimensional superficial maps of the tissue autofluorescence intensity and lifetime at three customizable emission bands with 100 µm lateral resolution. Both subsystems share the same excitation/illumination optical path and are simultaneously raster scanned on the sample to generate coregistered OCT volumes and FLIM images. The developed OCT/FLIM system was capable of a maximum A-line rate of 59 KHz for OCT and a pixel rate of up to 30 KHz for FLIM. The dual-modality system was validated with standard fluorophore solutions and subsequently applied to the characterization of two biological tissue types: postmortem human coronary atherosclerotic plaques, and in vivo normal and cancerous hamster cheek pouch epithelial tissue.read more
Citations
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Journal ArticleDOI
Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Rupsa Datta,Tiffany M. Heaster,Tiffany M. Heaster,Joe T. Sharick,Amani A. Gillette,Amani A. Gillette,Melissa C. Skala,Melissa C. Skala +7 more
TL;DR: FLIM is advantageous for probing molecular environments of fluorophores to inform on fluorophore behavior that cannot be elucidated with intensity measurements alone and development of FLIM technologies, analysis, and applications will further advance biological research and clinical assessments.
Journal ArticleDOI
Dental Optical Coherence Tomography
Yao-Sheng Hsieh,Yi-Ching Ho,Shyh Yuan Lee,Ching-Cheng Chuang,Jui-che Tsai,Kun Feng Lin,Chia-Wei Sun +6 more
TL;DR: The applications of dental optical coherence tomography in oral tissue images, caries, periodontal disease and oral cancer are described and comparisons between OCT and other clinical oral diagnostic methods are discussed.
Journal ArticleDOI
Intravascular Optical Imaging Technology for Investigating the Coronary Artery
Melissa J. Suter,Seemantini K. Nadkarni,Giora Weisz,Atsushi Tanaka,Farouc A. Jaffer,Brett E. Bouma,Brett E. Bouma,Guillermo J. Tearney,Guillermo J. Tearney +8 more
TL;DR: This review paper focuses on intravascular optical imaging modalities that provide information on the microstructural, compositional, biochemical, biomechanical, and molecular features of coronary lesions and stents.
Journal ArticleDOI
Optical coherence tomography--near infrared spectroscopy system and catheter for intravascular imaging.
Ali Fard,Paulino Vacas-Jacques,Ehsan Hamidi,Hao Wang,Robert W. Carruth,Joseph A. Gardecki,Guillermo J. Tearney +6 more
TL;DR: A dual-modality imaging system and catheter that uniquely combines IVOCT with diffuse near-infrared spectroscopy (NIRS) in a single dual- modality imaging device for simultaneous acquisition of microstructural and compositional information is presented.
Patent
Optical system and assay chip for probing, detecting and analyzing molecules
Jonathan M. Rothberg,Ali Kabiri,Sickler Jason W,Brett Gyarfas,Jeremy Lackey,Gerard Schmid,Benjamin Cipriany,Jack Jewell,Lawrence C. West,Ferrigno Michael,Paul E. Glenn,Adam E. Cohen,Anthony Bellofiore +12 more
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References
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Fluorescence lifetime imaging of free and protein-bound NADH.
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Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH
Damian K. Bird,Long Yan,Kristin M. Vrotsos,Kevin W. Eliceiri,Emily M. Vaughan,Patricia J. Keely,John G. White,Nirmala Ramanujam +7 more
TL;DR: A novel method for deriving functional maps of intracellular reduction-oxidation ratio in vivo via measurement of the fluorescence lifetimes and the ratio of free and protein-bound NADH using two-photon fluorescence lifetime imaging (FLIM).
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