Journal ArticleDOI
A Laminin and Nerve Growth Factor-Laden Three-Dimensional Scaffold for Enhanced Neurite Extension
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TLDR
Results indicate that DRG/PC 12 cell neurite outgrowth promoting effect of LN-modified agarose gels involves receptors for YIGSR/integrin beta1 subunits respectively and may find application as biosynthetic 3D bridges that promote regeneration across severed nerve gaps.Abstract:
Agarose hydrogel scaffolds were engineered to stimulate and guide neuronal process extension in three dimensions in vitro. The extracellular matrix (ECM) protein laminin (LN) was covalently coupled to agarose hydrogel using the bifunctional cross-linking reagent 1,19- carbonyldiimidazole (CDI). Compared to unmodified agarose gels, LN-modified agarose gels significantly enhanced neurite extension from three-dimensionally (3D) cultured embryonic day 9 (E9) chick dorsal root ganglia (DRGs), and PC 12 cells. After incubation of DRGs or PC 12 cells with YIGSR peptide or integrin beta1 antibody respectively, the neurite outgrowth promoting effects in LN-modified agarose gels were significantly decreased or abolished. These results indicate that DRG/PC 12 cell neurite outgrowth promoting effect of LN-modified agarose gels involves receptors for YIGSR/integrin beta1 subunits respectively. 1,2-bis(10, 12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC(8,9)PC)-based lipid microcylinders were loaded with nerve growth factor (NGF), and embedded into agarose hydrogels. The resulting trophic factor gradients stimulated directional neurite extension from DRGs in agarose hydrogels. A PC 12 cell-based bioassay demonstrated that NGF-loaded lipid microcylinders can release physiologically relevant amounts of NGF for at least 7 days in vitro. Agarose hydrogel scaffolds may find application as biosynthetic 3D bridges that promote regeneration across severed nerve gaps.read more
Citations
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Journal ArticleDOI
Engineering biocompatible implant surfaces
TL;DR: The authors in this paper reviewed the interdisciplinary field of biocompatible implant surfaces from the viewpoint of materials science, biochemistry and cell biology, and provided an overview on basic information about bulk and surface properties of implant surfaces.
Journal ArticleDOI
Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures
TL;DR: A physical model is postulate that predicts the rate of neurite extension in agarose gels, if gel stiffness is a known parameter, and this model is based on Heidemann and Buxbaum's model of neurites extension.
Book ChapterDOI
Polymers as biomaterials for tissue engineering and controlled drug delivery
TL;DR: This review highlights various biodegradable polymeric materials currently investigated for use in two key medical applications: drug delivery and tissue engineering.
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A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery
TL;DR: This review critically discusses the advances in biomaterial-based NGCs, their limitations and where future improvements may be required.
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Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin
TL;DR: Compared to covalent immobilization and physical adsorption, blended electrospinning of laminin and synthetic polymer is a facile and efficient method to modify nanofibers for the fabrication of a biomimetic scaffold.
References
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Corey S. Goodman,Carla J. Shatz +1 more
TL;DR: The range of activityindependent and activity-dependent mechanisms that generate precision of neuronal connections is considered-the connections between motoneurons and muscles and between the retina and higher visual centers--to highlight the extent to which different parts of the nervous system use the same mechanisms but in different proportions to achieve the final specificity.
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Journal ArticleDOI
Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding.
Jeannette Graf,Yukihide Iwamoto,Makoto Sasaki,George R. Martin,Hynda K. Kleinman,Frank A. Robey,Yoshihiko Yamada +6 more
TL;DR: A nearby peptide (CDPGYIGSR) from domain III with homology to epidermal growth factor was synthesized and found to be one of the principle sites in laminin mediating cell attachment, migration, and receptor binding.
Journal ArticleDOI
Characterization of the turning response of dorsal root neurites toward nerve growth factor
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A collagen-based nerve guide conduit for peripheral nerve repair: An electrophysiological study of nerve regeneration in rodents and nonhuman primates
TL;DR: This paper compares the results of a collagen nerve guide conduit to the more standard clinical procedure of nerve autografting to promote repair of transected peripheral nerves in rats and nonhuman primates.
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