A Mouse Erythroleukemia Cell Line Possessing Friend Spleen Focus‐forming Virus gp55 Transgene and Temperature‐sensitive Mutant p53 Gene

TLDR: This non‐virus‐producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erystrodifferentiation or apoptotic change.

Abstract: Two different erythroleukemia cell lines have been established from the splenic lesions of transgenic mice possessing the Friend spleen focus-forming virus (F-SFFV) gp55 gene. One showed a neardiploid karyotype and a temperature-sensitive (ts) p53 mutation, and the other, a hyper-triploid karyotype with double p53 mutations found by single-strand conformation polymorphism (SSCP) analysis. The cell lines both retained No.11 chromosomes on which p53 genes are localized. Another p53 allele in the cell line with the ts-p53 mutation appeared intact in the SSCP analysis of the genomic exon 5. The cells with the ts-mutant p53 gene showed no apparent change with temperature shift in their growth or dimethylsulfoxide-induced differentiation, although the wild-type p53 gene on the other allele was not expressing. This ts-p53val-135 gene made p53-deficient fibroblasts anchorageindependent at 37°C but not at 32°C. This non-virus-producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erythrodifferentiation or apoptotic change.