A MUTATION WHICH CHANGES A MEMBRANE PROTEIN OF E. coli
Masayori Inouye,J. P. Guthrie +1 more
TLDR
A temperature-sensitive mutant of E. coli, which cannot synthesize DNA at high temperature but can continue cell division, shows a difference in a membrane protein fraction at highTemperature by gel electrophoresis, which may link DNA replication and cell division through the membrane.Abstract:
A temperature-sensitive mutant of E. coli, which cannot synthesize DNA at high temperature but can continue cell division, shows a difference in a membrane protein fraction at high temperature by gel electrophoresis. This mutation of a bacterial membrane protein is unique. The protein may link DNA replication and cell division through the membrane.read more
Citations
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Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
TL;DR: This work states that the "SOS" Hypothesis, the Regulatory Role of DNA Damage in E. coli, and the Mechanism of SOS Repair in Bacteria and other UV-INDUCIBLE FUNCTIONS, and Regulations of SOS REPAIR, are valid hypotheses for the regulation of SOS functions.
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Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12.
TL;DR: Evidence is presented that penicillin bulge formation is due to the inhibition of proteins 2 and 3 in the absence of inhibition of protein 1.
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Solubilization of the Cytoplasmic Membrane of Escherichia coli by the Ionic Detergent Sodium-Lauryl Sarcosinate
TL;DR: In this paper, the sensitivity of the outer and cytoplasmic membranes of Escherichia coli to detergent was examined by isopycnic sucrose density gradient centrifugation.
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A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)
TL;DR: It is proposed that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it and this RNA interaction may cause premature termination of the transcription of theOmpF gene or destabilization of the OmpF RNA or both.
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Molecular determinants of bacterial adhesion monitored by atomic force microscopy
TL;DR: Atomic force microscopy can be used to analyze the initial events in bacterial adhesion with unprecedented resolution and a method for determining whether bacteria are attracted or repelled by virtually any biomaterial of interest is developed.
References
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Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
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An acrylamide gel soluble in scintillation fluid: Its application to electrophoresis at neutral and low pH
Choules Gl,Zimm Bh +1 more
TL;DR: Two additions to diacrylamide gel techniques are presented, one of which is the development of a catalyst system suitable for making pH 2.5 gels containing 1 M propionic acid as the electrolyte.
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Cell Division During Inhibition of Deoxyribonucleic Acid Synthesis in Escherichia coli
TL;DR: It was concluded that completion of a round of replication is a necessary and sufficient condition of DNA synthesis for cell division.
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Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r.
TL;DR: Investigation ofynchronous cultures of Escherichia coli strain B/r found that terminal steps in division can proceed in the absence of DNA synthesis, suggesting strongly that DNA synthesis is essential for cell division, and the end of a round of replication triggers cell division.
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Genetic Control of the Membrane Protein Component of the Lactose Transport System of Escherichia coli
TL;DR: Evidence that a membrane-bouind protein distinct from 0-galactosidase and thiogalactoside transacetylase (the two previously characterized proteins of the lac system) is an essential compouient of the transport system is reported, which indicates that the protein is coded by the genie of the Lac operon.