A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis
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TLDR
A genetic framework is established in which HsfA9 operates as a specialized Hsf for the developmental expression of Hsp genes during seed maturation, which is regulated by the seed-specific transcription factor ABSCISIC ACID–INSENSITIVE3.Abstract:
Within the Arabidopsis thaliana family of 21 heat stress transcription factors (Hsfs), HsfA9 is exclusively expressed in late stages of seed development. Here, we present evidence that developmental expression of HsfA9 is regulated by the seed-specific transcription factor ABSCISIC ACID–INSENSITIVE3 (ABI3). Intriguingly, ABI3 knockout lines lack detectable levels of HsfA9 transcript and protein, and further ectopic expression of ABI3 conferred the ability to accumulate HsfA9 in response to abscisic acid in transgenic plantlets. Consequently, the most abundant heat stress proteins (Hsps) in seeds (Hsp17.4-CI, Hsp17.7-CII, and Hsp101) were not detectable in the ABI3 knockout lines, but their expression could be detected in plants ectopically expressing HsfA9 in vegetative tissues. Furthermore, this seed-specific transcription factor cascade was reconstructed in transient β-glucuronidase reporter assays in mesophyll protoplasts by showing that ABI3 could activate the HsfA9 promoter, whereas HsfA9 in turn was shown to be a potent activator on the promoters of Hsp genes. Thus, our study establishes a genetic framework in which HsfA9 operates as a specialized Hsf for the developmental expression of Hsp genes during seed maturation.read more
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Complexity of the heat stress response in plants.
Sachin Kotak,Jane Larkindale,Ung Lee,Pascal von Koskull-Döring,Elizabeth Vierling,Klaus-Dieter Scharf +5 more
TL;DR: Transcriptome analysis of Arabidopsis has revealed the involvement of factors other than classical heat stress responsive genes in thermotolerance, suggesting both plant hormones and reactive oxygen species also contribute to heat stress signaling.
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Reactive oxygen signaling and abiotic stress.
TL;DR: Using mutants deficient in key ROS-scavenging enzymes, a signaling pathway is defined that is activated in cells in response to ROS accumulation and how ROS integrate different signals originating from different cellular compartments during abiotic stress is discussed.
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The plant heat stress transcription factor (Hsf) family: structure, function and evolution.
TL;DR: Data is compiled for 252 Hsfs from nine plant species (five eudicots and four monocots) with complete or almost complete genome sequences that provide interesting insights into phylogenetic relationships within the Hsf family in plants and allows the refinement of their classification into distinct groups.
Journal ArticleDOI
Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy
Kazuo Nakashima,Yasunari Fujita,Norihito Kanamori,Takeshi Katagiri,Taishi Umezawa,Satoshi Kidokoro,Kyonoshin Maruyama,Takuya Yoshida,Kanako Ishiyama,Masatomo Kobayashi,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki +11 more
TL;DR: Results indicate thatSRK2D, SRK2E and SRK 2I protein kinases involved in ABA signaling are essential for the control of seed development and dormancy through the extensive control of gene expression.
Journal ArticleDOI
Plant abiotic stress response and nutrient use efficiency.
Zhizhong Gong,Liming Xiong,Huazhong Shi,Shuhua Yang,Luis Herrera-Estrella,Luis Herrera-Estrella,Guohua Xu,Dai-Yin Chao,Jingrui Li,Peng Yun Wang,Feng Qin,Jijang Li,Yanglin Ding,Yiting Shi,Yu Wang,Yongqing Yang,Yan Guo,Jian-Kang Zhu +17 more
TL;DR: Recent work on the genetic and molecular mechanisms of plant abiotic stress and nutrient limitation sensing and signaling is summarized and new directions for future studies are discussed.
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