A point mutation in the Nul gene of bacteriophage λ facilitates phage growth in Escherichia coli with himA and gyrB mutations
TLDR
The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA: protein interactions that occur at the initiation of packaging.Abstract:
A mutant of lambda was isolated that grows in the Escherichia coli himA delta/gyrB-him320(Ts) double mutant at 42 degrees C; conditions which are non-permissive for wild-type lambda growth. The responsible mutation, ohm1, alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support lambda growth in the double himA/gyrB mutant, and lambda cos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA:protein interactions that occur at the initiation of packaging.read more
Citations
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Journal ArticleDOI
Integration host factor: a protein for all reasons.
TL;DR: The identification and characterization of the Escherichia coli DNA binding protein integration host factor (IHF) is an elegant example of how a well-characterized virus can be employed in the analysis of a host function.
Journal ArticleDOI
Global regulation of gene expression in Escherichia coli.
TL;DR: This new method for studying globally regulated genetic systems in E. coli combines detection, cloning, and physical mapping of a battery of coregulated genes in one step.
Journal ArticleDOI
Virus DNA packaging: the strategy used by phage lambda.
TL;DR: A key player in the lambda DNA packaging process is the phage-encoded enzyme terminase, which is involved in recognition of the concatemeric lambda DNA; initiation of packaging, which includes the introduction of staggered nicks at cosN to generate the cohesive ends of virion DNA and the binding of the prohead; and following translocation, the cutting of the terminal cosN, to complete DNA packaging.
Journal ArticleDOI
Growth phase variation of integration host factor level in Escherichia coli.
M D Ditto,D Roberts,R A Weisberg +2 more
TL;DR: Measurement of intracellular abundance of integration host factor (IHF), a site-specific, heterodimeric DNA-binding protein, in exponential- and stationary-phase cultures of Escherichia coli K-12 suggests that the wild-type exponential phase level is not in large excess of the minimum required for occupancy of physiologically important IHF-binding sites.
Journal ArticleDOI
Cell cycle-specific changes in nucleoprotein complexes at a chromosomal replication origin.
TL;DR: It is proposed that interplay between FIS and IHF aids assembly of initiation nucleoprotein complexes during the cell cycle and blocks initiation at inappropriate times.
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