Journal ArticleDOI
A polyamidoamine dendrimer-capped mesoporous silica nanosphere-based gene transfection reagent.
Daniela Rodica Radu,Cheng-Yu Lai,Ksenija Jeftinija,Eric W. Rowe,Srdija Jeftinija,Victor S.-Y. Lin +5 more
Reads0
Chats0
TLDR
The G2-PAMAM-capped MSN material (G2-MSN) was used to complex with a plasmid DNA (pEGFP-C1) that encodes for an enhanced green fluorescence protein that renders the possibility to serve as a universal transmembrane carrier for intracellular drug delivery and imaging applications.Abstract:
We synthesized a MCM-41-type mesoporous silica nanosphere (MSN)-based gene transfection system, where second generation (G2) polyamidoamines (PAMAMs) were covalently attached to the surface of MSN The G2-PAMAM-capped MSN material (G2-MSN) was used to complex with a plasmid DNA (pEGFP-C1) that encodes for an enhanced green fluorescence protein The gene transfection efficacy, uptake mechanism, and biocompatibility of the G2-MSN system with various cell types, such as neural glia (astrocytes), human cervical cancer (HeLa), and Chinese hamster ovarian (CHO) cells, were investigated The mesoporous structure of the MSN material allows membrane-impermeable molecules, such as pharmaceutical drugs and fluorescent dyes, to be encapsulated inside the MSN channels The system renders the possibility to serve as a universal transmembrane carrier for intracellular drug delivery and imaging applicationsread more
Citations
More filters
Journal ArticleDOI
Silica-based mesoporous organic-inorganic hybrid materials.
TL;DR: An overview of the preparation, properties, and potential applications of mesoporous organic-inorganic hybrid materials in the areas of catalysis, sorption, chromatography, and the construction of systems for controlled release of active compounds, as well as molecular switches, are given.
Journal ArticleDOI
Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers
TL;DR: This review highlights the recent research developments of a series of surface-functionalized mesoporous silica nanoparticle (MSN) materials as efficient drug delivery carriers and envision that these MSN-based systems have a great potential for a variety of drug delivery applications.
Journal ArticleDOI
Mesoporous materials for drug delivery.
TL;DR: This Minireview deals with the advances in this field by the control of the textural parameters, surface functionalization, and the synthesis of sophisticated stimuli-response systems.
Journal ArticleDOI
Mesoporous Silica Nanoparticles: Synthesis, Biocompatibility and Drug Delivery
TL;DR: The in vitro and in vivo biocompatibility and biotranslocation of MSNs are discussed in relation to their chemophysical properties including particle size, surface properties, shape, and structure.
Journal ArticleDOI
Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
References
More filters
Journal ArticleDOI
Poly(amidoamine) (PAMAM) dendrimers: from biomimicry to drug delivery and biomedical applications
Roseita Esfand,Donald A. Tomalia +1 more
TL;DR: This review focuses on crucial properties of biomimetic dendrimers that will broaden the potential for their use as macromolecular vectors in novel drug delivery and biomedical applications.
Journal ArticleDOI
A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro.
Carsten Kneuer,Mohammad Sameti,Udo Bakowsky,Thomas Schiestel,Hermann Schirra,Helmut Prof Dr Schmidt,Claus-Michael Lehr +6 more
TL;DR: The ability of colloidal silica particles with covalently attached cationic surface modifications to transfect plasmid DNA in vitro and the structure of the resulting transfection complexes are reported and described by analogy to the terms lipoplex and polyplex.
Journal ArticleDOI
Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers.
Jörg Dennig,Emma L. Duncan +1 more
TL;DR: Activated polyamidoamine (PAMAM)-dendrimers provide a new technology for gene transfer that offers significant advantages over classical methods and could be useful for in vivo gene transfer in gene therapy applications.
Journal ArticleDOI
Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein.
TL;DR: Green fluorescence is a quantitative measure of intracellular Gfp in single cells in spite of the dynamics of post-translational modifications involved in the conversion of expressed protein into its fluorescent form, according to a structured model formulated to describe the observed kinetics of gene expression and fluorophore formation.