Journal ArticleDOI
A rapid assay for neurotransmitter amino acids, aspartate, glutamate, glycine, taurine and γ-aminobutyric acid in the brain by high-performance liquid chromatography with electrochemical detection
TLDR
A very rapid and simple chromatographic method using high-performance liquid chromatography with electrochemical detection in combination with o-phthalaldehyde derivatization to measure amino acid neurotransmitter levels in several discrete brain regions of mice treated with and without electroconvulsive shock is described.Abstract:
For simultaneous assay of the five neurotransmitter amino acids, Asp, Glu, Gly, Tau, and GABA in brain tissues, a very rapid and simple chromatographic method using high-performance liquid chromatography with electrochemical detection in combination with o-phthalaldehyde derivatization is described. Because the present method permits the determination of these five amino acids within less than five minutes in one chromatographic run, up to 100 samples a working day can be analyzed using an autosampler. Withinrun coefficients of variation for these five amino acids were less than 2% (n=20). The quantitative detection limit was 2.5 pmol for the 5 amino acids. The present method has been applied to the measurement of the five amino acid neurotransmitter levels in several discrete brain regions of mice treated with and without electroconvulsive shock.read more
Citations
More filters
Journal ArticleDOI
The analysis of excitatory, inhibitory and other amino acids in rat brain microdialysates using microbore liquid chromatography
TL;DR: Three microbore liquid chromatography assays for determination of amino acids in rat brain dialysates are described, with a 5-fold increase in sensitivity for GABA and fast measurement of glutamate and aspartate.
Journal ArticleDOI
Rapid and sensitive step gradient assays of glutamate, glycine, taurine and γ-aminobutyric acid by high-performance liquid chromatography–fluorescence detection with o-phthalaldehyde–mercaptoethanol derivatization with an emphasis on microdialysis samples
TL;DR: In this article, a rapid step-gradient HPLC method was developed for determination of glutamate, glycine and taurine in striatal microdialysates using an automated refrigerated autoinjector.
Journal ArticleDOI
Effects of radiofrequency exposure on the GABAergic system in the rat cerebellum: clues from semi-quantitative immunohistochemistry.
TL;DR: Overall, it appears that high energetic radiofrequency exposure induces a diminution in cellular GABA content in the cerebellum.
Journal ArticleDOI
Levels of monoamine and amino acid neurotransmitters in the developing male mouse hypothalamus and in histotypic hypothalamic cultures.
TL;DR: The results shown in this study demonstrate that the monoamines appear early in the fetal brain and that the maximum expression of the catecholaminergic system, particularly that of dopamine, occurs during the late neonatal period or mouse infancy, when the role played by the ctcholamines on the maturation of the neurosecretory systems is more significant.
Journal ArticleDOI
d‐Aspartate as a putative cell–cell signaling molecule in the Aplysia californica central nervous system
TL;DR: D‐Asp is clearly detected in the major nerves of A.’californica, including the pleuroabdominal and cerebrobuccal connectives and the anterior tentacular nerves, suggesting it is transported long distances, and produces electrophysiological effects similar to those induced by l‐AsP.
References
More filters
Journal ArticleDOI
Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.
TL;DR: It is revealed that norepinephrine and dopamine are specifically localized in complex systems of neurons in the brain, a finding which lends support to the hypothesis that both amines may be neurotransmitters in the central nervous system.
Journal ArticleDOI
Amino acid neurotransmitters and their pathways in the mammalian central nervous system
G.E. Fagg,A.C. Foster +1 more
Journal ArticleDOI
An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates
TL;DR: An improved, HPLC with electrochemical detection method for the isocratic separation and determination of amino acids from post-mortem brain tissue and from microdialysates of awake-behaving animals is described.
Journal ArticleDOI
Regional gamma-aminobutyric acid levels in rat brain determined after microwave fixation.
TL;DR: GABA levels in rat whole brain were compared following three methods of sacrifice: rapid microwave fixation, decapitation into liquid nitrogen, and decapitation at 20°C, and the increase in GABA after decapitation was even greater in hypothalamus than in whole brain.
Journal ArticleDOI
O-Phtalaldehyde derivatives of amines for high-speed liquid chromatography/electrochemistry
TL;DR: Les amines peuvent etre analysees par chromatographie apres reaction avec l'o-phtalaldehyde and des thiols sont testes afin d'obtenir les derives les plus stables.