Journal ArticleDOI
A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei.
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TLDR
A dual-promoter approach is developed, for expressing highly toxic products and generating conditional gene knock-outs, using back-to-back constitutive T7 and tetracycline-responsive PARP promoters to drive expression of the selectable marker and test gene, respectively.About:
This article is published in Molecular and Biochemical Parasitology.The article was published on 1999-03-05. It has received 1351 citations till now. The article focuses on the topics: Regulation of gene expression & Heterologous expression.read more
Citations
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Journal ArticleDOI
Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters
TL;DR: A vector for in vivo tetracycline-inducible synthesis of double-stranded RNA (dsRNA) in stably transformed cells is described and one striking phenotype was the loss of kinetoplast DNA after interference with expression of a topoisomerase II.
Journal ArticleDOI
Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion.
TL;DR: A genetic screen to generate a tetracycline-inducible transactivator system in T. gondii caused severe impairment in host cell invasion and parasite spreading in cultured cells, and unambiguously established the pathogenic function of this motor in an animal model.
Journal ArticleDOI
Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
T. Nicolai Siegel,Doeke R. Hekstra,Louise E. Kemp,Luisa M. Figueiredo,Joanna E. Lowell,David Fenyö,Xuning Wang,Scott Dewell,George A. M. Cross +8 more
TL;DR: It is suggested that histone modifications and histone variants play crucial roles in transcription initiation and termination in trypanosomes and that destabilization of nucleosomes by hist one variants is an evolutionarily ancient and general mechanism of transcription initiation, demonstrated in an organism in which general pol II transcription factors have been elusive.
Journal ArticleDOI
A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei
Miguel Navarro,Keith Gull +1 more
TL;DR: It is proposed that the recruitment of a single expression site and the concomitant exclusion of inactive loci from a discrete transcriptional body define the mechanism responsible for VSG mono-allelic expression.
Journal ArticleDOI
Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites
TL;DR: This study demonstrates the usefulness of the RNA-seq technology to study the transcriptional landscape of an organism whose genome has not been fully annotated.
References
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Journal Article
Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication
R Brun,Schönenberger +1 more
Journal ArticleDOI
DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage.
TL;DR: The protein repair function of DnaK, GrpE and, in particular, DnaJ is likely to be part of the role of these proteins in regulation of the heat shock response.
Journal ArticleDOI
Mechanisms underlying expression of tn10 encoded tetracycline resistance
Wolfgang Hillen,Christian Berens +1 more
TL;DR: A detailed model of the repressor-operator complex has been proposed on the basis of biochemical and genetic data and demonstrated for the Tn10-encoded tet genes, which is the most sensitive effector-inducible system of transcriptional regulation known to date.
Journal ArticleDOI
Electroporation in ‘intracellular’ buffer increases cell survival
Journal ArticleDOI
Inducible gene expression in trypanosomes mediated by a prokaryotic repressor.
TL;DR: An inducible expression system was developed for the protozoan parasite Trypanosoma brucei that exhibited inducer (tetracycline)-dependent expression of chromosomally integrated reporter genes under the control of a procyclic acidic repetitive protein (PARP) promoter bearing a tet operator.
Related Papers (5)
Cultivation and in vitro cloning or procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Short communication
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