Journal ArticleDOI
Adaptation of Saccharomyces cerevisiae to growth on cholesterol: Selection of mutants defective in the formation of lanosterol
Frederick R. Taylor,L. W. Parks +1 more
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TLDR
The ability of cholesterol to support the growth of a heme-deficient mutant of Saccharomyces cerevisiae has been examined and this adaptation has proven to be the selection of spontaneous mutants in steps of the sterol biosynthetic pathway just prior to the formation of lanosterol.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1980-08-29. It has received 43 citations till now. The article focuses on the topics: Lanosterol & Ergosterol.read more
Citations
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Journal ArticleDOI
Multiple functions for sterols in Saccharomyces cerevisiae.
TL;DR: The critical domain role for sterol is observed under conditions of lanosterol supplementation where low levels of ergosterol (10-times those necessary for sparking on cholestanol) are required for growth.
Journal ArticleDOI
Defective sterol C5-6 desaturation and azole resistance: a new hypothesis for the mode of action of azole antifungals.
TL;DR: It is suggested that growth can occur through utilisation of 14-methyl fecosterol, produced by a combination of azole inhibition and defective sterol C5-6 desaturation.
Book ChapterDOI
Structure and Function of Sterols in Fungi
TL;DR: The structure and function of sterols in fungi is discussed, which shows that the pythiaceous fungi do not produce sterols because they cannot carry out the epoxidation of squalene, and it is believed that Plasmodiophoromycetes, which are obligate parasites, also do not production sterols.
Journal ArticleDOI
Biochemical approaches to selective antifungal activity. Focus on azole antifungals.
H. Vanden Bossche,Patrick Marichal,J. Gorrens,M.-C. Coene,G. Willemsens,D. Bellens,I. Roels,Henri Moereels,P. A. J. Janssen +8 more
TL;DR: Azole antifungals inhibit in fungal cells the 14α‐demethylation of lanosterol or 24–methylenedihydro‐lanosterol.
Journal ArticleDOI
Structural and physiological features of sterols necessary to satisfy bulk membrane and sparking requirements in yeast sterol auxotrophs.
Russell J. Rodriguez,L. W. Parks +1 more
TL;DR: Data are extended to hypothesize a role for the C-28 methyl group of ergosterol in yeast to satisfy bulk membrane and high-specificity (sparking) functions in three yeast sterol auxotrophs.
References
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Journal ArticleDOI
Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium
A. A. Andreasen,T. J. B. Stier +1 more
Journal ArticleDOI
Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene.
TL;DR: Tetrad analysis showed that the pleiotropic properties of each of the mutants resulted from a single mutation in one of five unlinked loci affecting heme biosynthesis, and each mutation resulted in loss of a single enzyme activity.
Journal ArticleDOI
The functional importance of structural features of ergosterol in yeast.
TL;DR: The data strongly suggest that the normal biosynthetic processes removal of methyl groups from the nucleus and introduction of one in the side chain are of functional significance and that the natural sterol probably acts functionally in the form of its preferred conformer in which C-22 is to the right ("right-handed") in the usual view.
Journal ArticleDOI
Unsaturated Fatty Acid Mutants of Saccharomyces cerevisiae
TL;DR: Two types of lipid nutritional mutants have been induced in this organism and one of these classes of mutants requires an unsaturated fatty acid and is associated with a locus on chromosome VII and the other class of mutants needs either an uns saturated fatty acid or ergosterol for growth.
Journal ArticleDOI
Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols.
J A Hossack,A H Rose +1 more
TL;DR: Cholesterol-enriched cells contained about 2% more lipid than cells enriched in any of the other sterols, which was largely accounted for by increased contents of triacylglycerols and, to a lesser extent, esterified sterols.