Journal ArticleDOI
Age-associated changes to pathogen-associated molecular pattern-induced inflammatory mediator production in dogs.
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TLDR
Whole blood from geriatric dogs had a blunted IL-10 response to LPS stimulation and middle-aged dogs had increased LPS-induced TNF production compared with the other groups.Abstract:
Objective – To determine whether older dogs will have a more pronounced pro-inflammatory response and blunted anti-inflammatory response to pathogen-associated molecular patterns (PAMPs) compared with younger dogs.
Design – Prospective.
Setting – University teaching hospital.
Animals – Thirty-eight privately owned sexually altered dogs of various ages.
Interventions – Blood was collected for HCT, WBC count, plasma biochemical analysis, and whole blood culture. Whole blood was stimulated with lipopolysaccharide (LPS) or, lipoteichoic acid or, peptidoglycan or, addition of phosphate-buffered saline. Tumor necrosis factor (TNF), interleukin (IL)-6, and IL-10 production from whole blood were compared among young, middle aged, and geriatric dogs.
Measurements and Main Results – LPS, lipoteichoic acid, and peptidoglycan stimulated significant TNF, IL-6, and IL-10 production from canine whole blood compared with phosphate-buffered saline. Whole blood from geriatric dogs had a blunted IL-10 response to LPS stimulation and middle-aged dogs had increased LPS-induced TNF production compared with the other groups.
Conclusion – PAMPs from gram-positive and gram-negative bacteria stimulate TNF, IL-6, and IL-10 production from canine whole blood. The inflammatory mediator response to PAMPs from gram-negative bacteria alters with age and may be one factor contributing to mortality in geriatric dogs with sepsis.read more
Citations
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Journal ArticleDOI
The Immunopathology of Sepsis: Pathogen Recognition, Systemic Inflammation, the Compensatory Anti‐Inflammatory Response, and Regulatory T Cells
TL;DR: The heterogeneous nature of this syndrome and the difficulty of modeling it in vitro or in vivo has both frustrated the advancement of new therapies and emphasized the continuing importance of patient‐based clinical research in this area of human and veterinary medicine.
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Plasma inflammatory mediator concentrations at ICU admission in dogs with naturally developing sepsis.
TL;DR: Plasma TNF, IL-6, CXCL-8, and IL-10 measured at ICU presentation do not appear to be valuable biomarkers to differentiate sepsis from NSIRS, or predict hospital outcome.
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Effects of freeze-dried cranberry powder on serum lipids and inflammatory markers in lipopolysaccharide treated rats fed an atherogenic diet.
TL;DR: It is suggested that freeze-dried cranberry powder may have beneficial effects on cardiovascular diseases by modifying serum lipids and the early inflammatory response.
Journal ArticleDOI
Understanding How Dogs Age: Longitudinal Analysis of Markers of Inflammation, Immune Function, and Oxidative Stress.
TL;DR: The findings indicate that aging dogs undergo changes similar to human inflammaging and offer the possibility of nutritional or pharmacological intervention to delay or reduce these effects.
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Biomarker Guided Diagnosis of Septic Peritonitis in Dogs.
Pia Martiny,Robert Goggs +1 more
TL;DR: The present study evaluated the ability of biomarkers to discriminate septic peritonitis from non-septic ascites (NSA) in dogs and found that dogs with SP had significantly greater blood CCL2 concentrations than dogs with NSA.
References
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Journal ArticleDOI
Inflammatory mediators in the elderly
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Journal ArticleDOI
Direct stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation.
D. De Groote,P. F. Zangerle,Y. Gevaert,M.F. Fassotte,Yves Beguin,F. Noizat-Pirenne,Jacques Pirenne,R. Gathy,M. Lopez,I. Dehart,D. Igot,M. Baudrihaye,Dominique Delacroix,Paul Franchimont +13 more
TL;DR: Diluted WB culture may be the most appropriate milieu in which to study cytokine production in vitro and individual variation in responses to PHA + LPS was always higher in PBMC cultures than in WB cultures.
Journal ArticleDOI
The Immunopathogenesis of Sepsis in Elderly Patients
TL;DR: The induction of proinflammatory cytokines after septic stimuli is not adequately controlled by anti-inflammatory mechanisms in elderly persons and this immune dysregulation is accompanied by a more pronounced procoagulant state in older patients.
Journal ArticleDOI
Markers to measure immunomodulation in human nutrition intervention studies
Ruud Albers,Jean-Michel Antoine,Raphaëlle Bourdet-Sicard,Philip C. Calder,Michael Gleeson,Bruno Lesourd,Sonia Samartín,Ian R. Sanderson,Jan Van Loo,F. Willem Vas Dias,Bernhard Watzl +10 more
TL;DR: It would be valuable to include several immune markers in addition to clinical outcome in future clinical trials in this area, as there is too little evidence that correlates markers with global health improvement.