Journal ArticleDOI
Allergens in bee venom. III. Identification of allergen B of bee venom as an acid phosphatase.
TLDR
Allergen B previously isolated from honeybee venom and shown to be a mildly acidic protein consisting of polymers of a chain of 49,000 d is shown to have acid phosphatase activity and is homogeneous by several criteria.Abstract:
Allergen B previously isolated from honeybee venom and shown to be a mildly acidic protein consisting of polymers of a chain of 49,000 d is shown to have acid phosphatase activity. Allergen B is homogeneous by several criteria. No acid phosphatase, alkaline phosphatase, or esterase activity was found in any other allergen or fraction of bee venom. Acid phosphatase activity was also found in yellow jacket venom and extracts of venom sacs from bumblebees and paper wasps.read more
Citations
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Insect allergy: The state of the art
TL;DR: Recommendations are derived from experience with the evaluation of over 500 patients and the therapy of over 300 adults and children, and a degree of controversy persists in this field, and additional data must be gathered before a fully defined position can be established.
Journal ArticleDOI
Immunochemical identification of the allergens in egg white
TL;DR: The sera of patients who experienced gastrointestinal symptoms from ingestion of egg white were less reactive with ovomucoid than sera from patients with other symptoms, and there was no significant association of pattern of reactivity with age, sex, or presence of eczema.
Journal ArticleDOI
Arthropod allergens and human health.
TL;DR: This chapter reviews the insects and mites that induce allergic reactions in humans and describes the methods for naming allergens.
Journal ArticleDOI
The protein composition of honeybee venom reconsidered by a proteomic approach.
Nico Peiren,Frank Vanrobaeys,Dirk C. de Graaf,Bart Devreese,Jozef Van Beeumen,Franciscus Jacobs +5 more
TL;DR: Honeybee venom samples submitted to two-dimensional gel electrophoresis resulted in the identification of 6 different known bee venom proteins and of 3 proteins that have not been described in such samples before.
Journal ArticleDOI
The nociceptive and anti-nociceptive effects of bee venom injection and therapy: A double-edged sword
Jun Chen,William R. Lariviere +1 more
TL;DR: Toxic bee venom injection may be beneficial for some patients, but may also be harmful, and key patterns of results, critical shortcomings, and essential areas requiring further study are highlighted.
References
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Journal ArticleDOI
A simplified method for cyanogen bromide activation of agarose for affinity chromatography
TL;DR: Agarose can be activated by adding cyanogen bromide, dissolved in acetonitrile, to beads suspended in a solution of sodium carbonate, and the necessity for manual titration and the use of a pH meter are eliminated.
Journal ArticleDOI
The Amino Acid Sequence of the γ Chain of Human Fetal Hemoglobin
TL;DR: The 146 amino acid residues of the γ chain of human fetal hemoglobin have been placed in sequence and the differences between the α and γ chains are responsible for the differences in the properties of the two molecules.
Journal ArticleDOI
Phospholipase A from bee venom.
Rudolf A. Shipolini,George L. Callewaert,Richard C. Cottrell,Shawn Doonan,Charles A. Vernon,Barbara E. C. Banks +5 more
TL;DR: The phospholipase A from the venom of the common European honey bee (Apis mellifica) has been completely purified and the identity of the enzyme as a phosphoripase of the A2 type has been confirmed.