Alterations in sperm characteristics of follicle-stimulating hormone (FSH)-immunized men are similar to those of FSH-deprived infertile male bonnet monkeys.
Hanumanthappa Krishnamurthy,K. M. Prasanna Kumar,Chirag V. Joshi,H. N. Krishnamurthy,Raghuveer N. Moudgal,M. Ram Sairam +5 more
TLDR
It appears that in monkeys and men, lack of FSH signaling results in production of sperm that exhibit defective chromatin packaging and reduction in acrosomal glycoprotein content, similar to that exhibited by sperm of some class of infertile men.Abstract:
The quality of sperm ejaculated by bonnet monkeys and normal, healthy proven fertile volunteer men, both actively immunized with ovine follicle-stimulating hormone (oFSH), was examined at different times of study for chromatin packaging and acrosomal glycoprotein concentration by flow cytometry. Susceptibility of sperm nuclear DNA to dithiothreitol (DTT)-induced decondensation, as measured by ethidium bromide binding, was markedly high compared with values at day 0 in men and monkeys during periods when FSH antibody titer was high. Sperm chromatin structure assay yields alphat values, which is another index of chromatin packaging. Higher alphat values, signifying poor packaging, occurred in both species following immunization with heterologous pituitary FSH. The binding of fluorosceinated pisum sativum agglutinin (PSA-FITC) to acrosome of sperm of monkeys and men was significantly low, compared with values at day 0 (control) during periods when cross-reactive FSH antibody titer was high and endogenous FSH was not detectable. Blockade of FSH function in monkeys by active immunization with a recombinant oFSH receptor protein corresponding to a naturally occurring messenger RNA (mRNA) also resulted in production of sperm with similar defects in chromatin packaging and reduced acrosomal glycoprotein concentration. Thus, it appears that in monkeys and men, lack of FSH signaling results in production of sperm that exhibit defective chromatin packaging and reduction in acrosomal glycoprotein content. These characteristics are similar to that exhibited by sperm of some class of infertile men. Interestingly, these alterations in sperm quality occur well ahead of decreased sperm counts in the ejaculate.read more
Citations
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The Functional Significance of FSH in Spermatogenesis and the Control of Its Secretion in Male Primates
Tony M. Plant,Gary R. Marshall +1 more
TL;DR: An integrative analysis of the role of FSH in the control of testicular function in higher primates, including man is provided, with the presentation of a model for the operation of the FSH-inhibin B feedback control system regulating sperm production postpubertally in monkey and man.
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Male germ cell apoptosis: regulation and biology
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Qualitative and Quantitative Decline in Spermatogenesis of the Follicle-Stimulating Hormone Receptor Knockout (FORKO) Mouse
TL;DR: The data allow us to conclude that genetic disruption of FSH receptor signaling in the rodent induces major changes that might contribute to reduced fertility, and sperm from FORKO males are susceptible to acid denaturation, indicating the poor quality of sperm.
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Primate spermatogenesis: new insights into comparative testicular organisation, spermatogenic efficiency and endocrine control.
TL;DR: This review aims at providing new data and insights into comparative primate spermato‐genesis, dealing specifically with quantitative aspects of germinal epithelial organisation and germ cell production, and with the roles of gonadotrophic hormones in this process.
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Delay in Sexual Maturity of the Follicle-Stimulating Hormone Receptor Knockout Male Mouse
TL;DR: It is suggested that the FORKO mouse might be a useful experimental model to define the molecular mechanisms that underlie the delay in puberty, as it was followed from Day 7 onward by using histology and quantitative DNA flow cytometry.
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