Ambient Temperature Signal Feeds into the Circadian Clock Transcriptional Circuitry Through the EC Night-Time Repressor in Arabidopsis thaliana
Takeshi Mizuno,Yuji Nomoto,Haruka Oka,Miki Kitayama,Aya Takeuchi,Mayuka Tsubouchi,Takafumi Yamashino +6 more
TLDR
Evidence is provided that temperature signals feed into the clock transcriptional circuitry through the evening complex (EC) night-time repressor consisting of EARLY FLOWERING 3 (ELF3, ELF4) and LUX ARRHYTHMO (LUX; also known as PCL1), which revealed the sophisticated physiological mechanism underlying the clock-controlled output pathway, which leads to the PIF4-mediated temperature-adaptive regulation of hypocotyl elongation.Abstract:
An interlocking multiloop model has been generally accepted to describe the transcriptional circuitry of core clock genes, through which robust circadian rhythms are generated in Arabidopsis thaliana. The circadian clock must have the ability to integrate ambient temperature signals into the clock transcriptional circuitry to regulate clock function properly. Clarification of the underlying mechanism is a longstanding subject in the field. Here, we provide evidence that temperature signals feed into the clock transcriptional circuitry through the evening complex (EC) night-time repressor consisting of EARLY FLOWERING 3 (ELF3, ELF4) and LUX ARRHYTHMO (LUX; also known as PCL1). Chromatin immunoprecipitation assays showed that PSEUDO-RESPONSE REGULATOR7 (PRR7), GIGANTEA (GI) and LUX are direct targets of the night-time repressor. Consequently, transcription of PRR9/PRR7, GI and LUX is commonly regulated through the night-time repressor in response to both moderate changes in temperature (Δ6°C) and differences in the steady-state growth-compatible temperature (16-28°C). A warmer temperature inhibits EC function more, whereas a cooler temperature stimulates it more. Consequently, the expression of these target genes is up-regulated in response to a warm temperature specifically during the dark period, whereas they are reversibly down-regulated in response to a cool temperature. Transcription of another EC target, the PIF4 (PHYTOCHROME-INTERACTING FACTOR 4) gene, is modulated through the same thermoregulatory mechanism. The last finding revealed the sophisticated physiological mechanism underlying the clock-controlled output pathway, which leads to the PIF4-mediated temperature-adaptive regulation of hypocotyl elongation.read more
Citations
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Phytochromes function as thermosensors in Arabidopsis
Jaehoon Jung,Mirela Domijan,Cornelia Klose,Surojit Biswas,Daphne Ezer,Mingjun Gao,Asif Khan Khattak,Mathew S. Box,Varodom Charoensawan,Sandra Cortijo,Manoj Kumar,Alastair Grant,James C. W. Locke,Eberhard Schäfer,Katja E. Jaeger,Philip A. Wigge +15 more
TL;DR: A major thermosensory role for the phytochromes (red light receptors) during the night is described, and it is found that phy tochrome B directly associates with the promoters of key target genes in a temperature-dependent manner.
Journal ArticleDOI
Molecular and genetic control of plant thermomorphogenesis
Marcel Quint,Marcel Quint,Carolin Delker,Carolin Delker,Keara A. Franklin,Philip A. Wigge,Karen J. Halliday,Martijn van Zanten +7 more
TL;DR: How the emerging knowledge in Arabidopsis may be transferred to relevant crop systems is discussed, as this knowledge will be key to rational breeding for thermo-tolerant crop varieties.
Journal ArticleDOI
A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis.
Jaehoon Jung,Jaehoon Jung,Antonio Daniel Barbosa,Stephanie Hutin,Janet R. Kumita,Mingjun Gao,Dorothee Derwort,Catarina S. Silva,Xuelei Lai,Xuelei Lai,Elodie Pierre,Feng Geng,Sol-Bi Kim,Sujeong Baek,Chloe Zubieta,Katja E. Jaeger,Katja E. Jaeger,Philip A. Wigge,Philip A. Wigge,Philip A. Wigge +19 more
TL;DR: It is shown that ELF3 proteins in plants from hotter climates, with no detectable PrD, are active at high temperatures, and lack thermal responsiveness, which represents a previously unknown thermosensory mechanism.
Journal ArticleDOI
Molecular Regulation of Plant Responses to Environmental Temperatures
TL;DR: Recent progress is discussed in exploring thermomorphogenesis, vernalization, and the mechanisms underlying plant tolerance to extreme temperatures, which are crucial for understanding the interactions between plants and temperature.
Journal ArticleDOI
Genetic and epigenetic control of plant heat responses
TL;DR: This review focuses on recent progresses regarding the genetic and epigenetic control of heat responses in plants, and pays more attention to the role of the major epigenetic mechanisms in plant heat responses.
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