Journal ArticleDOI
Amino acid and soluble protein cocktail from waste keratin hydrolysed by a fungal keratinase of Paecilomyces marquandii
Mária Veselá,Jožefa Friedrich +1 more
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TLDR
Comparison with other protein hydrolysates aimed for fertilizer suggests that keratin degradation products, obtained by enzymatic hydrolysis, have potential to be used for foliar fertilization, alone or in a combination with another complementary hydrolysate of a different source, such as skin or plant proteins.Abstract:
Waste bovine hooves and horns were enzymatically hydrolysed into soluble products intended for foliar fertilizer. With the powdered keratin at 50°C and pH 8 between 34 to nearly 60% of nitrogen was solubilized in 5 h, depending on the enzyme concentration. The reaction could further be improved by steam pretreatment of the keratin, resulting in 98% solubilisation of the nitrogen. The products of hydrolysis consisted of a mixture of soluble proteins, peptides, and free amino acids. Among the latter, 18 common amino acids were detected. Several of them were previously recognized to have a positive effect on plants. Nonpolar neutral, basic, and sulphur amino acids were present in relatively large amounts, while proline and tryptophan were not found. Comparison with other protein hydrolysates aimed for fertilizer suggests that keratin degradation products, obtained by enzymatic hydrolysis, have potential to be used for foliar fertilization, alone or in a combination with another complementary hydrolysate of a different source, such as skin or plant proteins.read more
Citations
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Biostimulants in Plant Science: A Global Perspective.
TL;DR: This review suggests that the focus of biostimulant research and validation should be upon proof of efficacy and safety and the determination of a broad mechanism of action, without a requirement for a specific mode of action.
Journal ArticleDOI
The amazing potential of fungi: 50 ways we can exploit fungi industrially
Kevin D. Hyde,Jianchu Xu,Jianchu Xu,Sylvie Rapior,Rajesh Jeewon,Saisamorn Lumyong,Allen Grace Niego,Allen Grace Niego,Pranami D. Abeywickrama,Janith V. S. Aluthmuhandiram,Rashika S. Brahamanage,Siraprapa Brooks,Amornrat Chaiyasen,K. W. Thilini Chethana,Putarak Chomnunti,Clara Chepkirui,Boontiya Chuankid,Nimali I. de Silva,Mingkwan Doilom,Mingkwan Doilom,Mingkwan Doilom,Craig B. Faulds,Eleni Gentekaki,Venkat Gopalan,Pattana Kakumyan,Dulanjalee Harishchandra,Hridya Hemachandran,Sinang Hongsanan,Anuruddha Karunarathna,Anuruddha Karunarathna,Samantha C. Karunarathna,Sehroon Khan,Jaturong Kumla,Ruvishika S. Jayawardena,Jian-Kui Liu,Ning-Guo Liu,Thatsanee Luangharn,Allan Patrick G. Macabeo,Diana S. Marasinghe,Dan Meeks,Peter E. Mortimer,Peter E. Mortimer,Peter Mueller,Sadia Nadir,Sadia Nadir,Karaba N. Nataraja,Sureeporn Nontachaiyapoom,Meghan O’Brien,Watsana Penkhrue,Chayanard Phukhamsakda,Uma Shaanker Ramanan,Achala R. Rathnayaka,Resurreccion B. Sadaba,Birthe Sandargo,Binu C. Samarakoon,Danushka S. Tennakoon,Ramamoorthy Siva,Wasan Sriprom,Trichur S. Suryanarayanan,Kanaporn Sujarit,Nakarin Suwannarach,Thitipone Suwunwong,Benjarong Thongbai,Naritsada Thongklang,De-Ping Wei,S. Nuwanthika Wijesinghe,Jake Winiski,Jiye Yan,Erandi Yasanthika,Marc Stadler +69 more
TL;DR: This manuscript reviews fifty ways in which fungi can potentially be utilized as biotechnology and provides a flow chart that can be used to convince funding bodies of the importance of fungi for biotechnological research and as potential products.
Journal ArticleDOI
Biochemical features of microbial keratinases and their production and applications.
TL;DR: Keratinases are exciting proteolytic enzymes that display the capability to degrade the insoluble protein keratin and their use in biomass conversion into biofuels may address the increasing concern on energy conservation and recycling.
Journal ArticleDOI
Microbial enzymes for bioconversion of poultry waste into added-value products
TL;DR: Main areas for further studies are large scale production and purification of keratinases, and development of effective processes for production of bioactive molecules from poultry waste.
Journal ArticleDOI
Alternative Methods of Preparation of Soluble Keratin from Chicken Feathers
TL;DR: In this paper, the effect of various reducing agents, such as 2-mercaptoethanol, dithiothreitol, sodium m-bisulphite, and sodium bisulphitic, as well as sodium hydroxide on the yield of chicken feathers was determined.
References
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Journal ArticleDOI
Investigation of the bicinchoninic acid protein assay: Identification of the groups responsible for color formation
TL;DR: Electrochemical studies and the magnitude of the color changes observed when the reaction is carried out at 37 degrees C indicate that tryptophan, tyrosine, and the peptide bond are not completely oxidized at this temperature.
Journal ArticleDOI
Preferential use of organic nitrogen for growth by a non-mycorrhizal arctic sedge
TL;DR: This paper showed that Eriophorum vaginatum, an arctic sedge, rapidly absorbs free amino acids, accounting for at least 60% of the nitrogen absorbed by this species in the field.
Journal ArticleDOI
Microbial keratinases and their prospective applications: an overview.
Rani Gupta,Priya Ramnani +1 more
TL;DR: Keratinases stand out among proteases since they attack the keratin residues and hence find application in developing cost-effective feather by-products for feed and fertilizers and their prospective application in the challenging field of prion degradation would revolutionize the protease world in the near future.
Journal ArticleDOI
A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources
TL;DR: Supporting evidence of a nutritional (amino acid) upgrading sequel to diverse microbial treatments of feathers, and positive results obtained from growth studies in rats and chicks have been presented.
Journal ArticleDOI
Purification and Characterization of a Keratinase from a Feather-Degrading Bacillus licheniformis Strain
TL;DR: The purified keratinase hydrolyzes a broad range of substrates and displays higher proteolytic activity than most proteases and is a useful enzyme for promoting the hydrolysis of feather keratin and improving the digestibility of feather meal.
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