Journal ArticleDOI
Amperometric hydrogen peroxide biosensor with sol-gel/chitosan network-like film as immobilization matrix.
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TLDR
A new type of sol-gel/organic hybrid composite material based on the cross-linking of natural polymer chitosan with (3-aoryloxypropyl) dimethoxymethylsilane was developed for the fabrication of an amperometric H( 2)O(2) biosensor that retained approximately 75% of its original activity after about 60 days of storage in a phosphate buffer at 4 degrees C.About:
This article is published in Biosensors and Bioelectronics.The article was published on 2003-04-01. It has received 207 citations till now. The article focuses on the topics: Biosensor.read more
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Chitosan chemistry and pharmaceutical perspectives.
TL;DR: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. S. Nagar, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of medicine, Jerusalem 91120, Israel.
Journal ArticleDOI
Application of chitin- and chitosan-based materials for enzyme immobilizations: a review
TL;DR: A review of the literature on enzymes immobilized on chitin- and chitosan-based materials, covering the last decade, is presented in this paper, where one hundred fifty-eight papers on 63 immobilized enzymes for multiplicity of applications ranging from wine, sugar and fish industry, through organic compounds removal from wastewaters to sophisticated biosensors for both in situ measurements of environmental pollutants and metabolite control in artificial organs, are reviewed.
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A glucose biosensor based on chitosan–glucose oxidase–gold nanoparticles biocomposite formed by one-step electrodeposition
TL;DR: The combination of gold nanoparticles affinity and the promising feature of the biocomposite with the onestep nonmanual technique favor the sensitive determination of glucose with improved analytical capabilities.
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An amperometric cholesterol biosensor based on multiwalled carbon nanotubes and organically modified sol-gel/chitosan hybrid composite film.
TL;DR: Results show that analytical performance of the biosensor can be improved greatly after introduction of the MWCNTs, and this method has been used to determine the free cholesterol concentration in real human blood samples.
Journal ArticleDOI
Electrochemically deposited chitosan hydrogel for horseradish peroxidase immobilization through gold nanoparticles self-assembly.
TL;DR: A new strategy for immobilization of horseradish peroxidase (HRP) has been presented by self-assembling gold nanoparticles on chitosan hydrogel modified Au electrode, which displayed an excellent catalytic property to the reduction of H2O2 in the presence of methylene blue mediator.
References
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Journal ArticleDOI
Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped Bands
TL;DR: In this article, the Fourier self-deconvolution (FDS) method was proposed to resolve overlapped lines that can not be instrumentally resolved due to their intrinsic linewidth.
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Crystal structure of horseradish peroxidase C at 2.15 A resolution.
TL;DR: The crystal structure of horseradish peroxidase isozyme C (HRPC) has been solved to 2.15 Å resolution and the key residue involved in direct interactions with aromatic donor molecules is identified, predicted to be important for the ability of HRPC to bind aromatic substrates.
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Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer
TL;DR: The low K app M values demonstrated that Horseradish peroxidase immobilized on Au colloids exhibited a high affinity to H 2 O 2 with no loss of enzymatic activity.
Journal ArticleDOI
Direct Electrochemistry of Horseradish Peroxidase Immobilized on a Colloid/Cysteamine-Modified Gold Electrode
TL;DR: Direct electron transfer of immobilized horseradish peroxidase on gold colloid and its application as a biosensor showed an excellent electrocatalytic response to the reduction of H( 2)O(2) without the aid of an electron mediator and showed a Michaelis-Menten behavior at higher H(2).
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Hydrogen peroxide and .beta.-nicotinamide adenine dinucleotide sensing amperometric electrodes based on electrical connection of horseradish peroxidase redox centers to electrodes through a three-dimensional electron relaying polymer network
TL;DR: The sensitivity of the resulting H202 cathode at O.OV(SCE) is 1Acm(-2)M(-1) and its current increases linearly with H2O2 concentration in the 1x10(-7)M(-)2x10(4)M range as discussed by the authors.
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Direct Electrochemistry of Horseradish Peroxidase Immobilized on a Colloid/Cysteamine-Modified Gold Electrode
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