Journal ArticleDOI
An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH
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TLDR
A model for variable region gene rearrangement mediated by proteins which recognize the same conserved sequences adjacent to both light and heavy chain immunoglobulin gene segments is proposed.About:
This article is published in Cell.The article was published on 1980-04-01. It has received 736 citations till now. The article focuses on the topics: Immunoglobulin gene & Conserved sequence.read more
Citations
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Journal ArticleDOI
Somatic generation of antibody diversity
TL;DR: In the genome of a germ-line cell, the genetic information for an immunoglobulin polypeptide chain is contained in multiple gene segments scattered along a chromosome which are assembled by recombination which leads to the formation of a complete gene.
Journal ArticleDOI
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
TL;DR: The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
Journal ArticleDOI
Single amino acid substitution altering antigen-binding specificity
TL;DR: Results suggest that small numbers of substitutions in antibodies, such as those presumably introduced by somatic mutation, may in some situations be effective in altering antigen-binding specificity.
Book ChapterDOI
Somatic Generation of Antibody Diversity1
TL;DR: In this paper, it was shown that an organism does not inherit even a single complete gene for antibody polypeptide chains, rather, the genetic information is transmitted in germline as no more than several hundred gene segments.
Journal ArticleDOI
A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes
TL;DR: It is proposed that this tissue-specific enhancer contributes to the activation of somatically rearranged immunoglobulin variable region genes and possibly to abnormal expression of other genes (e.g. c-myc) that become translocated to its domain of influence.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Screening lambdagt recombinant clones by hybridization to single plaques in situ
WD Benton,Ronald W. Davis +1 more
TL;DR: A rapid, direct method for screening single plaques of Agt recombinant phage is described, which allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
Journal ArticleDOI
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
TL;DR: In this article, it was shown that in vitro completion of these nascent light chains resulted in the synthesis of some chains having the same mol wt as the authentic secreted light chain, because of completion of in vivo proteolytically processed chains and of other chains which, due to the completion of unprocessed chains, have the same moll wt, as the precursor of the light chain.
Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
Journal ArticleDOI
An analysis of the sequences of the variable regions of bence jones proteins and myeloma light chains and their implications for antibody complementarity
TL;DR: It is postulated that the information for site complementarity is contained in some extrachromosomal DNA such as an episome and is incorporated by insertion into the DNA of the structural genes for the variable region of short linear sequences of nucleotides.