Journal ArticleDOI
An improved method for thin-layer chromatography of nucleotide mixtures containing32P-labeled orthophosphate
TLDR
Developing poly(ethylene)imine cellulose thin layers with phosphate solutions gives improved resolution of complex mixtures of nucleotides and minimizes the tailing of highly radioactive orthophosphate present in the mixtures and thus facilitates chromatographic analysis of crude acid extracts of phosphate-labeled bacteria.About:
This article is published in Journal of Chromatography A.The article was published on 1969-01-01. It has received 332 citations till now. The article focuses on the topics: Thin-layer chromatography & Nucleotide.read more
Citations
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The Control of Ribonucleic Acid Synthesis in Escherichia coli: IV. RELEVANCE OF UNUSUAL PHOSPHORYLATED COMPOUNDS FROM AMINO ACID-STARVED STRINGENT STRAINS
TL;DR: Comparison of properties of the system regulating the synthesis of these compounds with those of the amino acid control of RNA biosynthesis suggests that the unusual compounds participate in an early step in the mechanism which leads to the slowing ofRNA biosynthesis during amino acid starvation of stringent strains.
Journal ArticleDOI
Steps in the processing of Ad2 mRNA: Poly(A)+ Nuclear sequences are conserved and poly(A) addition precedes splicing
TL;DR: These data on conservation of the Ad2 sequences suggest that each transcriptional event from the 16-99 transcription unit gives rise to one of a possible 13-14 mRNA molecules with destruction of the remainder of the transcribed RNA.
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Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
TL;DR: Small ribonucleic acid (RNA)-protein complexes precipitated by anti-Ro and anti-La antibodies from lupus patients have been examined with emphasis on their RNA components and the possibility that the La protein(s) functions in the transcription or maturation of all RNA polymerase III transcripts is discussed.
Journal ArticleDOI
The Control of Ribonucleic Acid Synthesis in Escherichia coli V. CHARACTERIZATION OF A NUCLEOTIDE ASSOCIATED WITH THE STRINGENT RESPONSE
Michael Cashel,Barbara Kalbacher +1 more
TL;DR: Charge considerations, enzyme degradation studies, and labilities in acid and alkali lead to a structural assignment for MS I of guanosine 5'-diphosphate or 3'- or 2'-dphosphate (ppGpp).
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Purified |[lambda]| regulatory protein cII positively activates promoters for lysogenic development
H Simatake,Mara Rosenberg +1 more
TL;DR: The bacteriophage λ regulatory protein, cII, has been purified and shown to activate positively RNA transcription from the two phage promoters which coordinately regulate phage lysogenic development.
References
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Journal ArticleDOI
Ion-exchange thin-layer chromatography: XIII. Resolution of complex nucleoside triphosphate mixtures☆
TL;DR: CTP, dCTP, UTB, dTTP, ATP, dATP, GTP, DTP, and ITP can be completely separated from each other and from other mononucleotides by two-dimensional anion-exchange thin-layer chromatography on PEI-cellulose.
Journal ArticleDOI
Resolution of complex nucleotide mixtures by two-dimensional anion-exchange thin-layer chromatography.
TL;DR: Very complex mononucleotide mixtures can be resolved by two-dimensional anion-exchange chromatography on poly(ethyleneimine)-cellulose thin layers because the method is more sensitive than paper chromatography and column chromatography of nucleotides.
Journal ArticleDOI
Control of RNA synthesis in Escherichia coli. I. Amino acid dependence of the synthesis of the substrates of RNA polymerase.
Michael Cashel,Jonathan Gallant +1 more
TL;DR: It is suggested that the RC control system responds to amino acid deficiency by limiting ribonucleoside triphosphate synthesis, which in turn makes RNA synthesis amino acid-dependent.