Analysis of fluorescence decay kinetics measured in the frequency domain using distributions of decay times
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TLDR
The theoretical and practical aspects of analyzing complex fluorescence decay kinetics using continuous distributions of decay times using frequency-domain data, provides for global analysis of multiple data sets and includes the possibility of excited-state processes are described.About:
This article is published in Biophysical Chemistry.The article was published on 1987-10-01 and is currently open access. It has received 106 citations till now. The article focuses on the topics: Branching fraction & Relaxation (NMR).read more
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LUMINESCENCE SPECTRAL PROPERTIES OF CdS NANOPARTICLES
TL;DR: In this paper, the steady state and time resolved luminescence spectral properties of two types of novel CdS nanoparticles and nanoparticles are described and a new constant positive polarized emission from luminescent nanoparticles is also described.
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Ligand binding and protein relaxation in heme proteins: a room temperature analysis of nitric oxide geminate recombination
Jacob W. Petrich,Jean-Christophe Lambry,Krzysztof Kuczera,Martin Karplus,Claude Poyart,Jean-Louis Martin +5 more
TL;DR: The present results for ligand binding in heme proteins and its time and temperature dependence are discussed and it appears likely that, at low temperatures, inhomogeneous protein populations play an important role and that as the temperature is raised, relaxation effects become significant as well.
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A 10‐GHz frequency‐domain fluorometer
Gabor Laczko,Ignacy Gryczynski,Zygmunt Gryczynski,Wiesław Wiczk,Henryk Malak,Joseph R. Lakowicz +5 more
TL;DR: In this article, the authors describe the design and performance of a 10 GHz harmonic-content frequency-domain fluorometer with 6 μm channels, where the modulated excitation is provided by the harmonic content of a train of ps pulses.
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A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin.
TL;DR: A cysteine engineering approach is used to create a high resolution, FRET-based sensor that reports a large, approximately 70 degree nucleotide-dependent angle change of the light-chain binding region and shows that bound Mg.ADP.Pi induces the myosin to adopt the prestroke states.
References
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Exposure of tryptophanyl residues in proteins. Quantitative determination by fluorescence quenching studies.
TL;DR: The value of this probing technique lies in its ability to sense not only the steady-state exposure of a residue in a protein, but also its dynamic exposure.
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Simultaneous analysis of multiple fluorescence decay curves: A global approach
TL;DR: In this paper, a procedure for simultaneous analysis of multiple fluorescence decay curves is described, which exploits relationships between individual decays, and results in increased model testing sensitivity and more accurate parameter recovery.
Journal ArticleDOI
A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution
Enrico Gratton,M. Limkeman +1 more
TL;DR: A detailed description of the construction and performance of a variable frequency cross-correlation phase fluorometer is reported and some application to the measurement of multiexponential decays is illustrated.
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Analysis of fluorescence decay kinetics from variable-frequency phase-shift and modulation data
TL;DR: Analysis of fluorescence phase-shift and modulation data by the method of nonlinear least squares to determine the values of the lifetimes and fractional intensities for a mixture of exponentially decaying fluorophores broadly confirms the predicted resolving potential of variable-frequency phase-modulation fluorometry.